Develop a pet radiotracer suitable for in vivo imaging of tgfβ

Ended Project Grant Awarded Last action

Award ID
R03CA283104
Recipient
Awarding agency
Obligated
$170,000

Award facts

Assistance listing
93.394 Cancer Detection and Diagnosis Research
Completion date
Place of performance
Portland, OR 97213, USA

Award details come from USAspending.gov.

Description

As the agency reported it.

Abstract
Transcription growth factor beta (tgfβ) is a pleiotropic cytokine with a significant role in creating an immunosuppressive tumor microenvironment. High levels of tgfβ are strongly associated with poor cancer prognosis, but there are currently no non-invasive methods available to quantify the cytokine in vivo. This proposal describes the design and evaluation of a novel biologic positron emission tomography (pet) radiotracer to selectively and quantitatively detect tgfβ in vivo. The proposed biologic construct is a recombinant protein incorporating the extracellular domain of human tgfβ receptor II (tβrii) linked to the FC fragment of human IGG2 via the antibody hinge region. This recombinant protein was further modified by appending 1,4,7- triazacyclononane-1,4,7-triacetic acid (nota) copper chelator via a p-SCN-BN reactive moiety. The resulting precursor molecule will be labeled with 64CU to produce the active radiotracer, 64CU-FC:tβrii. The 64CU-FC:tβrii radiotracer will be evaluated for preclinical efficacy by imaging mice using small animal pet-MRI. This proposal aims to assess the efficacy of 64CU-FC:tβrii including a dose titration experiment to determine the optimal dosage and incubation time for the radiotracer, a blocking study, a comparison of in vivo pet standardized uptake values (suvs) with EX vivo tumor lysate tgfβ concentrations in four murine tumor models, and an experiment to detect changes in tgfβ expression before and after radiation therapy.

Quick answers

Who received award R03CA283104?

R03CA283104 was awarded to Providence Health & Services - Oregon by National Cancer Institute (National Institutes of Health) on July 1, 2024.

How much is award R03CA283104 worth?

$170,000 has been obligated on R03CA283104.

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