This R01 project grant from the National Heart, Lung, and Blood Institute (CFDA 93.837 Cardiovascular Diseases Research) will provide $728,651 to the Masonic Medical Research Lab (doing business as Masonic Medical Research Institute) between September 2024 and June 2029. The project aims to utilize a novel murine model of pulmonary embolism to investigate the inflammatory and fibrotic response through longitudinal molecular imaging. The research will examine the effects of emboli age, size, and location on downstream complications like pulmonary hypertension and recurrent embolism. A sub-award to The Research Foundation for the State University of New York will support single-nucleus RNA sequencing and spatial transcriptomic analysis of murine lung tissues to profile cellular changes in response to the pulmonary emboli.