The National Cancer Institute (NCI) awarded a $699,080 Project Grant under the 21st Century Cures Act - Beau Biden Cancer Moonshot program (CFDA 93.353) to ANN & Robert H Lurie Children's Hospital of Chicago. The purpose of this 5-year grant, awarded on July 23, 2024, is to implement Optical Genome Mapping (OGM) as a first-line diagnostic approach for pediatric brain tumors. The funding will enable the hospital to utilize OGM to rapidly identify structural genomic variants with potential clinical significance in a large cohort of pediatric brain tumor samples. This research aims to improve diagnosis, outcome prediction, and ultimately advance therapies for children with brain tumors. The award will also support the validation of OGM as a CLIA/CAP certified diagnostic test for pediatric brain tumors in the hospital's pathology laboratories and the submission of a laboratory developed test application.