THE PURPOSE OF THIS PROJECT IS TO IMPROVE PUBLIC HEALTH PROTECTION THROUGH THE DEVELOPMENT OF QUANTITATIVE DATA ON THE REMOVAL OF HUMAN ENTERIC VIRUSES BY TREATMENT PROCESSES WHERE WASTEWATER IS THE SOURCE WATER. RESEARCHERS WILL QUANTIFY THE REMOVAL OF SELECTED HUMAN ENTERIC VIRUSES BY DIFFERENT TREATMENT TRAINS AND PROCESSES AT FIVE FULL-SCALE WATER REUSE FACILITIES ACROSS THE UNITED STATES. ACTIVITIES INCLUDE MONITORING FIVE FULL-SCALE TREATMENT FACILITIES FOR VIRUS AND SURROGATE REMOVAL AFTER VARIOUS TREATMENT PROCESSES, THEN VALIDATING THE USEFULNESS OF SURROGATES FOR ASSIGNING LOG REDUCTION VALUES FOR THE DIFFERENT TREATMENT PROCESSES. DELIVERABLES AND OUTCOMES FROM THIS STUDY INCLUDE A TOOLBOX WITH, AT A MINIMUM, STANDARDIZED PROTOCOLS FOR DETECTION OF PATHOGENIC VIRUSES, APPLICATION OF VIRUS LOG REDUCTION VALUES AND SURROGATES, AND EXAMPLES OF HOW THE RESULTS OF THIS PROJECT CAN BE USED IN PRACTICE TO PROTECT PUBLIC HEALTH BY PROVIDING ROBUST VIRUS LOG REDUCTION VALUE CREDITS F