Awards issued prior to january 20, 2025, were funded under previous administrations and may not reflect the priorities and policies of the current administration.** increasing atmospheric…

Awards issued prior to january 20, 2025, were funded under previous administrations and may not reflect the priorities and policies of the current administration.** increasing atmospheric CO2CONCENTRATIONS has the potential to improveagricultural output. However, the photosynthetic gains from rising atmospheric CO2CONCENTRATIONS are frequently much lower than the maximum predicted gains. This discrepancy is the result of a down-regulation of photosynthesis that is connected to plant carbon-nitrogen imbalance when grown at elevated CO2. While this phenomenon occurs in legumes, IT is not as severe as severe dueto their ability to exchange carbon for nitrogen with symbiotic soil bacteria. Understanding how legumes sense, integrate, and respond to nitrogen and carbon/energy status through dynamic control of gene expression will enable the development of strategies to achieve maximum crop yield and quality in future climates.to accomplish this, we willbuild gene regulatory networks that combine gene expression datawith epigenomic data measured across nitrogen and CO2TREATMENTS. We hypothesize that because epigenetic/chromatin states are known to influence transcription, their inclusion will enhance the accuracy of our predictions and improve our ability to identify key transcription factors involved in coordinating carbon-nitrogen homeostasis. This approach has broad potential in identifying regulatory factors in a broad range of signaling pathways and eukaryotic species, ultimately, the regulators of carbon nitrogen balanceidentified will be important for breeding and/or developing transgenic lines with increased yield and seed quality, specifically focusing on strategies that will be robust to future climates.