Developing cell type-specific enhancers and connectivity mapping pipelines for marmosets
Active Cooperative Agreement Awarded Last action
- Award ID
- UH3MH126869
- Recipient
-
Massachusetts Institute Of Technology UEI E2NYLCDML6V1
- Awarding agency
-
National Institute of Mental Health
National Institutes of Health, Department of Health and Human Services
- Obligated
- $2,836,783
Award facts
- Assistance listing
- 93.242 Mental Health Research Grants
- Completion date 88% complete
- Place of performance
- Cambridge, MA 02139, USA
Award details come from USAspending.gov.
Description
- Abstract
- Although genetic tools have dramatically advanced our understanding of brain function, they have largely been confined to mice. While mice are essential models for many areas of neuroscience, there are also many aspects of higher brain function that cannot be adequately modeled in rodents. Similarly, many brain disorders affect higher cognitive functions that have no clear parallels in rodents. Furthermore, recent large- scale single cell transcriptomic analyses have revealed many neuron types, connections and gene expression patterns that are unique to primates. Thus, there is an urgent need for new genetic models that have brain structure and function closer to humans. Non-human primates (NHP) are much more closely related to humans than are rodents, and this is reflected in their brain development, structure and physiology. Hence, IT is increasingly recognized that they provide an attractive model to study higher brain function and brain disorders. A promising emerging NHP model is the common marmoset, a small new world primate that has many advantages for neuroscience and genetic research. However, lack of tools with cell type specificity has been a major obstacle in advancing structural and functional studies in NHP. With the combined single cell RNA-SEQ and single cell atac-SEQ, IT is now possible to nominate short cell type-specific enhancer sequences. If validated, these enhancers will provide an effective tool to map connectivity and interrogate function using virus mediated expression. The difficulty lies in the identification of functional enhancers from the hundreds or thousands of nominated potential enhancer sequences in NHP. Here we propose (1) to use a novel high throughput in vivo approach to identify functional enhancers, and (2) to establish a whole-brain circuit mapping pipeline for use striatal circuitry to validate our approach for cell type-specific connectivity mapping in marmosets. When completed, these studies will provide much needed essential tools, methods and computational pipelines for cell type-specific mapping and functional interrogation of the marmoset brain in healthy and disease models.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $1,406,867 | |
| Original award | $1,429,916 |
Subawards
| Subrecipient | Amount | Awarded |
|---|---|---|
| Cold Spring Harbor Laboratory | $419,392 | |
| The Broad Institute, Inc. | $121,701 | |
| The Trustees Of Princeton University | $199,367 |
Quick answers
Who received award UH3MH126869?
UH3MH126869 was awarded to Massachusetts Institute Of Technology by National Institute of Mental Health (National Institutes of Health) on September 1, 2021.
How much is award UH3MH126869 worth?
$2,836,783 has been obligated on UH3MH126869.
On GovTribe
See this award's full record
- Every transaction and subaward
- The recipient's other awards and teaming partners
- Similar grant awards
- Ask GovTribe AI about this award