X-RAY nanotomography for scalable connectomics
Active Cooperative Agreement Awarded Last action
- Award ID
- U01NS142093
- Recipient
-
The Francis Crick Institute LIMITED UEI VRHEWNUW2PS5
- Awarding agency
-
National Institute of Neurological Disorders and Stroke
National Institutes of Health, Department of Health and Human Services
- Obligated
- $2,019,316
Award facts
- Assistance listing
- 93.372 21st Century Cures Act - Brain Research through Advancing Innovative Neurotechnologies
- Completion date 38% complete
- Place of performance
- Great Britain, United Kingdom
Award details come from USAspending.gov.
Description
- Abstract
- Current gold-standard methods for dense tissue reconstruction rely on volume electron microscopy. As electron penetration depth into tissue is limited to a few 100S of NM, this requires tissue sectioning or milling, presenting significant scalability challenges for reliable continuous image acquisition. Importantly, while automatic segmentation methods have seen substantial improvements in recent years, manual proof-reading still provides a significant cost and time overhead that is particularly driven by the interfaces of physical sections. X-rays penetrate macroscopic samples, allowing for largely non-destructive imaging of tissues. Large scale synchrotron facilities can provide high-brightness coherent x-rays at wavelengths in the sub-nanometer range, enabling coherent imaging with nanometer scale 3D resolution. Recent developments in synchrotron technology have seen further increase in coherent photon flux by orders of magnitude at "4TH generation" synchrotrons. Theoretical calculations have demonstrated that this puts x-RAY imaging of whole mouse brains with 20 NM isotropic resolution in reach. Here, we will leverage the first high-energy realisation of such a 4TH generation source at the european synchrotron research facility (esrf). Our proposal is based on the recent decision of esrf to dedicate a new beamline to the development of x-RAY connectomics, aiming for routine acquisition of MM3 volumes with 20 NM resolution in
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Amendment | $90,868 | |
| Amendment | $90,868 | |
| Amendment | $918,790 | |
| Original award | $918,790 |
Quick answers
Who received award U01NS142093?
U01NS142093 was awarded to The Francis Crick Institute LIMITED by National Institute of Neurological Disorders and Stroke (National Institutes of Health) on September 1, 2025.
How much is award U01NS142093 worth?
$2,019,316 has been obligated on U01NS142093.
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