Low-cost crispr-on-paper for cervical cancer screening at the point of care

Active Cooperative Agreement Awarded Last action

Award ID
U01CA269147
Recipient
Awarding agency
Obligated
$7,766,096

Award facts

Assistance listing
93.394 Cancer Detection and Diagnosis Research
Completion date 90% complete
Place of performance
Farmington, CT 06032, USA

Award details come from USAspending.gov.

Description

As the agency reported it.

Abstract
Cervical cancer is among the leading causes of cancer death in women worldwide, especially in low- and middle- income countries (LMICS). High-risk human papillomaviruses (HPV) are the main causative agents of cervical cancer and its precursor lesions; therefore, the world health organization (who) has recommended HPV DNA testing for cervical cancer screening in LMICS. Although polymerase chain reaction (PCR) methods have been widely used for HPV DNA detection, they are restricted to centralized clinical laboratories due to the need for labor-intensive procedures and expensive equipment. Here, we propose to develop a simple, rapid, highly sensitive, and specific CRISPR-on-paper diagnostic platform to simultaneously detect multiple high-risk HPV genotypes for cervical cancer screening at the point of care. This innovative diagnostic system is based on our recently developed all-in-one dual CRISPR-CAS12A (AIOD-CRISPR) assay, which combines the simplicity and high sensitivity of isothermal nucleic acid amplification with the high specificity of CRISPR detection. To develop a low-cost, multiplexed molecular detection technology, we will incorporate the AIOD-CRISPR assay into a paper-based microfluidics platform. To eliminate the need for complex electronic instruments, we will take advantage of an exothermic reaction to generate chemical heat for the CRISPR-on-paper system by using a disposable hand warmer, thus enabling instrument-free cervical cancer screening. The detection results can be read by the naked eye or reported by a programmed smartphone without the need for an expensive optical detector. We will rigorously evaluate and validate the clinical applications of our CRISPR-on-paper diagnostic system by testing clinical samples in collaboration with clinicians and healthcare workers in uconn health and zambia. If successful, the proposed project has an important impact on global health by providing a simple, affordable, and sensitive method for rapid screening of cervical cancer in resource-poor settings. As a platform technology, the proposed CRISPR-on-paper diagnostic system can be easily adapted to detect other emerging pathogens.

Transactions

Action Obligated Date
Amendment $66,400
Amendment $442,788
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Amendment $66,400
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Amendment -$12,384
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Amendment $486,145
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Amendment $505,996
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Amendment $520,639
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Original award $547,265
Show all 44

Subawards

Subrecipient Amount Awarded
Centre For Infectious Disease Research In Zambia The UConn Health lab will collaborate with the PI at the Central Laboratory at Centre for Infectious Disease Research in Zambia (CIDRZ) in this U01 application.… $66,400
Board Of Regents Of Nevada System Of Higher Education The micro/nanofluidic laboratory at the University of Nevada Las Vegas will carry out the tasks assigned to it in the proposal. Specifically, the UNLV team will… $57,576
Centre For Infectious Disease Research In Zambia The Centre for Infectious Disease will: i) ensure all regulatory approvals for this study, ii) enroll study participants including screening, collecting, and… $59,790
Board Of Regents Of Nevada System Of Higher Education The micro/nanofluidic laboratory at the University of Nevada Las Vegas will carry out the tasks assigned to it in the proposal. Specifically, the UNLV team will… $51,818
Centre For Infectious Disease Research In Zambia Centre for Infectious Disease will: i) ensure all regulatory approvals for this study, ii) enroll study participants including screening, collecting, and… $59,760
Board Of Regents Of Nevada System Of Higher Education The micro/nanofluidic laboratory at the University of Nevada Las Vegas will carry out the tasks assigned to it in the proposal. Specifically, the UNLV team will… $54,697

Quick answers

Who received award U01CA269147?

U01CA269147 was awarded to University Of Connecticut Health Center by National Cancer Institute (National Institutes of Health) on May 1, 2022.

How much is award U01CA269147 worth?

$7,766,096 has been obligated on U01CA269147.

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