AI-driven biomarker analysis of intact whole brains imaged at micron and sub-micron resolution

Ended Project Grant Awarded Last action

Award ID
R43MH125512
Recipient
Awarding agency
Obligated
$1,121,498

Award facts

Assistance listing
93.242 Mental Health Research Grants
Completion date
Funding
  • $894,744.00 Federal
  • $226,754.00 Non-federal
Place of performance
East Cambridge, MA 02141, USA

Award details come from USAspending.gov.

Description

As the agency reported it.

Abstract
Whole-organ 3D immunohistochemistry is revolutionizing the field of neuroscience, enabling unprecedented insight into the distribution of neural cells and neurological markers throughout the brain in health and disease. Lifecanvas technologies is at the forefront of the new field of spatial proteomics, providing a complete workflow for whole-organ preservation, tissue clearing, immunohistochemical labeling, and imaging. Nevertheless, an ongoing challenge for such studies is the need to rapidly, reproducibly and rigorously quantify terabyte-sized datasets from whole-organ imaging efforts. While progress has been made in applying artificial intelligence (AI) tools to enable detection of cellular and sub-cellular markers in neural tissue, one-size-fits-all algorithms are inadequate for analyzing complex, information-rich brain datasets due to varying biomolecular expression patterns (e.g. nuclear, cytoplasmic, membrane-bound) and region-specific heterogeneities in cell density and neural cell types. However, AI-driven algorithms targeting a subset of labeling patterns can be effective provided the availability of adequate training data. Lifecanvas technologies LCT is optimally positioned to develop highly accurate algorithms serving a wide range of detection tasks through its access to high volumes of whole-organ image data containing a variety of label expression patterns via its contract research organization and user base. LCT proposes to develop a data analysis program, smartanalytics, which will embed a suite of AI algorithms within a user-friendly software package to identify labeled cell locations and characterize morphological features across the whole brain at cellular and sub-cellular resolution. Specifically, LCT will use intact, 3D immunolabeled mouse brains to design AI algorithms to detect labeled cells imaged at cellular resolution and generate further algorithms for the segmentation of labeled features imaged at sub-micron resolution. Data from LCT's contract research organization and academic collaborations will be continually FED back to improve and expand the library of detection algorithms available within smartanalytics, and these developments will drive further customer adoption and enhancement of future versions of the software. Smartanalytics will guide users through model application, quality-control testing, and the generation of output products such as figures and summary statistics. In summary, smartanalytics will be an evolving and user- friendly workflow execution program that enables neuroscientists to take full advantage of their 3D image data, driving new discoveries in brain function, development and disease.

Transactions

Action Federal Non-federal Date
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Amendment $220,618 $0
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Amendment $226,754 $0
Original award $226,754 $226,754

Quick answers

Who received award R43MH125512?

R43MH125512 was awarded to Lifecanvas Technologies Inc. by National Institute of Mental Health (National Institutes of Health) on February 1, 2021.

How much is award R43MH125512 worth?

$1,121,498 has been obligated on R43MH125512: $894,744 in federal funding and $226,754 in non-federal funding.

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