Conduct surveys for pathogens of… (AP26PPQFO000C471) – Cooperative Agreement to University Of Guam

Purpose: The purpose of this agreement is to conduct surveys for pathogens of solanaceous commodities at local farms and distribution centers.
Activities: Symptomatic plant samples shall be collected for further confirmation with molecular diagnostic assays. Agdia immunostrips will be used for confirmation of ralstonia solanacearum race 3 biovar 2 and tobamovirus tomato brown rugose fruit virus. Orthotospovirus groundnut BUD necrosis virus will be confirmed with agdia elisa kits (highly sensitive diagnostic tools) candidatus phytoplasma australiense 16SRXII-b will be confirmed with traditional polymerase chain reaction (PCR) and sequencing of suggested markers from peer reviewed publication. Deliverables and expected outcomes: - RE-initiation of solanaceous plant pathology surveys previously conducted by university of guam (UOG); - increased capacity in UOG-western pacific tropical research center (wptrc) to detect pathogens via molecular diagnostics; - creation of a database at UOG of negative data to support future export market development efforts and quarantine negotiations with regional governments. Intended beneficiary(IES): nurseries/plant production facilities, plant RE-sellers, the general public/consumers of nursery products. Subrecipient activities, if know n or specified at the time of award: no subawards. Financials: personnel = $41,840; travel = $1,250; supplies = $6,343; indirect = $7,415

Conduct surveys for pathogens of solanaceous commodities at local farms and distribution centers