IT is imperative to have a healthy pollinator supply if we wish to continue to produce pollinator dependent fruit, nuts, and vegetables in this country

Honeybee health challenges are attributable to parasites, diseases, and environmental toxins. Therefore, aphis is funding a national BEE survey to document which diseases/parasites/pests of honeybees are and are not present in the U.S. Survey efforts will also support U.S. Honeybee queen producers' requests for the opening of and/or continued access to foreign markets such as canada. Robust data, such as past and proposed efforts, will provide data needed to remove trade barriers. Activities to be performed - using the methods published on the aphis website, samples will be taken to allow US to confidently verify the absence of certain exotic pests including tropilaelaps mites and the asian honeybee. In collaboration with the agriculture research service (ARS) BEE research laboratory, molecular techniques will be used to quantify the presence of the BEE viruses listed in table 1. Microscopic techniques will be used to determine nosema ceranae levels, and established diagnostic methods will be used to quantify varroa destructor. Deliverables and expected outcomes: the 2025 national honeybee pests and diseases survey sampling in each participating state will be divided into two sections, 1) longitudinal sampling of 5 beekeepers, and 2) 14 general survey surveillance samples split into 3 or more sampling trips throughout the year. Because the longitudinal sampling should be conducted twice for each of the 5 selected beekeepers per state, each state should have a total of 24 samples at the end of the sampling season intended beneficiary(IES): results from these samples will be provided to the state plant health director, state plant regulatory official, state survey coordinator, state apiary specialist, and the beekeeper(S) who provided the sample. Beekeepers receiving these reports will be able to make data informed management decisions. Honeybee health is at risk from factors such as parasites, diseases, poor nutrition, stress, and environmental toxins. The national honeybee pests and diseases survey data is used to ascertain the scope of PAR asites, diseases, and pests that may have a negative impact on honeybee populations in the U.S. This nation-wide survey is the most comprehensive honeybee pest and health survey to date and provides essential disease and pest load information. Subrecipient activities, if known or specified at the time of award: none.