In vitro and in vivo experimental evolution of eubacterial rnase p we explore the catalytic versatility and adaptability of the RNA component of a critical ribonucleoprotein, rnase p. Using in vitro…
Ended Grant For Research Awarded Last action
- Contract number
- NNX08AE90G
- Awardee
-
The Trustees Of The Smith College UEI KRDJLRA9X6F3
- Awarding agency
-
Shared Services Center
National Aeronautics and Space Administration
- Obligated
- -$1
Award facts
- Contract value 0% obligated
- $134,999.22 Current and potential, with all options
- Completion date 100% complete
- Current and potential, with all options
- Funding agency
- Headquarters National Aeronautics and Space Administration
- NAICS code
- Not on record
- Set-aside
- No set-aside
- Competition
- Not Competed · 0 offers Only One Source
- Pricing
- Other
- Place of performance
- Northampton, MA 01063, USA
Award details come from SAM.gov/FPDS.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Funding Only Action | -$1 | |
| Other Administrative Action | $0 | |
| Other Administrative Action | $0 | |
| Other Administrative Action | $0 |
Description
In vitro and in vivo experimental evolution of eubacterial rnase p we explore the catalytic versatility and adaptability of the RNA component of a critical ribonucleoprotein, rnase p. Using in vitro evolution, we evolve a descendant of e. Coli rnase p RNA capable of cleaving a DNA substrate. The ability to cleave this substrate involves a tradeoff between catalytic specialization and the ability of rnasep to process multiple RNA substrates, a tradeoff that may be mitigated by the presence of the C5 protein moiety of rnasep. We explore this tradeoff, and its implications for the evolution of novel catalytic activities. We also propose a series of in vivo experiments that result in the DE novo evolution of an rnasep RNA capable of complementing a deletion of the gene (rnpb) encoding the RNA moiety of this ribonucleoprotein in e. Coli. The results will shed light on the topography ofthe adaptive landscape of this ribozyme, and identify the structural elements and sequence motifs critical to catalytic function. Finally, we envision a parallel set of experiments that explore the evolutionary landscape of the protein moiety of rnasep, and that examine the interplay between overall gene expression (the "transcriptome") and the catalytic activity of a single component of metabolism.
Quick answers
Who was awarded contract NNX08AE90G?
NNX08AE90G was awarded to The Trustees Of The Smith College by Shared Services Center (National Aeronautics and Space Administration) on August 22, 2011.
How much is contract NNX08AE90G worth?
The government has obligated -$1 on NNX08AE90G. Its current value, with exercised options, is $134,999.
Which agency awarded contract NNX08AE90G?
Shared Services Center (National Aeronautics and Space Administration) awarded NNX08AE90G, with funding from Headquarters (National Aeronautics and Space Administration).
Was contract NNX08AE90G competed?
The extent of competition for NNX08AE90G was not competed. The government received 0 offers.
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