ECOAnalyst Bid 01519.pdf

EXHIBIT A-2 – BIDDER’S PROFILE PAGE 1

(Rev 2019-07-01)

EXHIBIT A-2 – BIDDER’S PROFILE

Competitive Solicitation No.: 01519-2

Bidder: EcoAnalysts, Inc.

BIDDER INFORMATION

Legal name (from Business License) and address:

Business Name

Address City, State, Zip Code

EcoAnalysts, Inc.

4729 NE View Dr.

Port Gamble, WA 98364

Washington State Department of Revenue Registration Number Note: This is the Unified Business Identifier (UBI)

Federal Tax ID No. (TIN):

Note: If your TIN is a Social Security number, provide only the last four digits.

Is your firm certified as a minority or woman owned business with the Washington State Office of Minority & Women’s Business Enterprises

(OMWBE)?

Yes No If yes, provide MWBE certification no.

Is your firm a self-certified Washington State small business?

Note: See definitions of ‘microbusiness,’ ‘minibusiness,’ and ‘small business,” set forth in

RCW 39.26.010.

Yes No

If yes, what is your business size?

Small Mini Micro

Is your firm certified as Veteran Owned with the Washington State Department of Veteran Affairs?

Yes No

If yes, provide WSDVA certification no.

82-0476170

602609395

EXHIBIT A-2 – BIDDER’S PROFILE PAGE 2

CONTRACT MANAGEMENT POINTS OF CONTACT

Authorized Representative Name: Brian Hester Email: bhester@ecoanalysts.com Phone: 360-297-6040 x6045

Contract Administrator Name: Regina Edwards Email: redwards@ecoanalysts.com Phone: 360-297-6040 x6040

Sales Reporting Representative Name: Brian Hester Email: bhester@ecoanalysts.com Phone: 360-297-6040 x6045

Sales Reporting Alternate Name: Regina Edwards Email : redwards@ecoanalysts.com Phone: 360-297-6040 x6040

Management Fee Representative Name: Brian Hester Email: bhester@ecoanalysts.com Phone: 360-297-6040 x6045

Management Fee Contact Alternate Name: Regina Edwards Email: redwards@ecoanalysts.com Phone: 360-297-6040 x 6040

Address for Enterprise Services to send management fee invoices:

Company name: EcoAnalysts, Inc.

Attn: Regina Edwards Address: PO Box 216 City/State/Zip: Port Gamble, WA 98364

ORDERING/SALES POINTS OF CONTACT (expand as necessary)

Name Phone Number E-mail Area of Responsibility

Brian Hester 360-461-5784 bhester@ecoanalysts.com Bioassay

Julia Baum 360-509-4141 jbaum@ecoanalysts.com Bioassay mailto:bhester@ecoanalysts.com mailto:redwards@ecoanalysts.com mailto:redwards@ecoanalysts.com mailto:bhester@ecoanalysts.com mailto:jbaum@ecoanalysts.com bhester Cross-Out

EXHIBIT A-2 – BIDDER’S PROFILE PAGE 3

REFERENCES

Provide a minimum of three (3) commercial or government references for which bidder has delivered goods and/or services similar in scope as described in the Competitive Solicitation.

Reference 1

Company Name:

Contact:

Phone:

Email:

Anchor QEA Cheronne Oreiro 206-903-3310 coreiro@anchorqea.com

Reference 2

Company Name:

Contact:

Phone:

Email:

USACE Seattle District David Fox 206-764-6083 david.f.fox@usace.army.mil

Reference 3

Company Name:

Contact:

Phone:

Email:

City of Gig Harbor Wastewater Plant Norine Alvarado 253-851-8999 AlvaradoN@cityofgigharbor.net

WILL CALL/SERVICE LOCATIONS

Identify will call or service locations throughout the state.

Location Point of Contact Phone Number Area(s) of Responsibility

PURCHASE CARDS (I.E., CREDIT CARDS)

Please indicate which types of purchasing (credit) cards are accepted (note: any card fees must be included in the unit price of the bid):

Visa Master Card American Express Discover Other:

STATE OF OREGON – USE OF MASTER CONTRACT

This contract will be available to certain additional eligible purchasers in the State of Oregon (our ORCPP partners).

Bidder agrees to provide goods/services pursuant to the Master Contract to ORCPP members? Yes No

If required, indicate percent increase to bid pricing for delivery to ORCPP members: 3 percent.

EXHIBIT A-2 – BIDDER’S PROFILE PAGE 4

Return this Bidder’s Profile to Procurement Coordinator at:

DESCPRMENVIRONPROC@des.wa.gov

01519 Analytical Laboratory Services Exhibit B Evaluation Questions

EXHIBIT B – NON-COST EVALUATION QUESTIONS

Competitive Solicitation No.: 01519-2

Bidder: EcoAnalysts, Inc.

The Evaluation portion will have a maximum value of 35 points towards the total scoring. Each question is worth 5 points. Do not put your company name in the answers.

Question Answer

Provide most recent 3 Proficiency Testing results for the analyses/methods/matrix of interest

DMRQA -38, DMRQA-37, and DMRQA-36 Reports Attached.

Provide sample data packages for samples of similar types to those to be analyzed. The data packages should include initial calibration data.

Example Report Attached. Calibration data for equipment used for bioassay are maintained onsite.

Provide Method Detection Limit values for the analytes/methods of interest

Method Detection Limit values do not apply for bioassay parameters

Provide Initial Demonstration of Capability data for the analytes/methods of interest

Initial demonstration of capability data are reviewed through our accreditation process and are maintained onsite.

How many years of experience analyzing samples of similar types to those to be analyzed 21

Provide your Limit of Quantitation including reporting units for each Method bid. Not applicable for bioassay parameters

Provide copies of current Accreditation certificates and fields of testing. WDOE and NELAP Accreditations Attached.

Julia Baum EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

USA

DMR-QA Study

Open Date: 03/22/19

Close Date: 07/19/19

Report Issued Date: 08/16/19

DMRWet-39 Final Report

Study # : DMRWet-39

Ver. 1

August 16, 2019

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

Enclosed is your final report for ERA's DMRWet 39 Proficiency Testing study. Your final report includes an evaluation of every result submitted by your facility to ERA.

All analytes in ERA's DMRWet 39 Proficiency Testing study have been evaluated by comparing the reported result to the acceptance limits generated using the criteria contained in the most current TNI FoPT tables.

If you have any "Not Acceptable" evaluations for the DMRWet 39 study, please contact ERA's Customer Service Group at 1-800-372-0122 for your corrective action needs.

Thank you for your participation in ERA's DMRWet 39 Proficiency Testing study. If you have any questions regarding the study, please contact our Proficiency Testing Department at 1-800-372-0122.

Sincerely, Matthew Seebeck Quality Officer attachments

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-39

Report Recipient Contact/Phone Number Reporting Type Evaluation Type

California ELAP Christine Sotelo / 916-341-5175 All Analytes 2009 TNI

California WRB (DMRQA) Renee Spears / 916-341-5583 All Analytes 2009 TNI

Louisiana Paul Bergeron / 225-219-3247 All Analytes 2009 TNI

Oregon Stephanie Ringsage / 503-693-4126 All Analytes 2009 TNI

Washington Rebecca Wood / 360-871-8811 All Analytes 2009 TNI

Study # : DMRWet-39

DMRWet-39 Definitions & Study Discussion Study Dates: 03/22/19 - 07/19/19 Report Issued: 08/16/19

DMRWet Study Definitions DMRWet Study Discussion

The Performance Evaluation:

Acceptable

Not Acceptable

No Evaluation

Reported Value falls within the Acceptance Limits.

Reported Value falls outside the Acceptance Limits.

Reported Value cannot be evaluated.

ERA's DMRWet 39 Proficiency Testing study has been reviewed by ERA senior management and certified compliant with the requirements of the 2009 TNI PT Standards and the criteria contained in the most current TNI FoPT tables.

ERA's DMRWet 39 study standards were examined for any anomalies. A full review of all homogeneity, stability and accuracy verification data was completed. All analytical verification data for all analytes met the acceptance criteria contained in ththe 2009 TNI PT Standard and the criteria contained in the most current TNI Fields of Proficiency Testing (FoPT) tables.

The data submitted by participating laboratories was also examined for study anomalies. There were no anomalies observed during the statistical review of the data.

ERA's DMRWet 39 study reports shall not be reproduced except in their entirety and not without the permission of the participating laboratories. The report must not be used by the participating laboratories to claim product endorsement by any agency of the U. S. government.

The data contained herein are confidential and intended for your use only.

If you have any questions or concerns regarding your assessment in ERA's DMRQA Proficiency Testing program, please contact our Proficiency Testing Department at 1-800- 372-0122.

The Method Description is the method the laboratory reported to ERA.

The Reported Value is the value that the laboratory reported to ERA.

The Assigned Values for NOEC Analytes are set to the Study Median of the data reported by laboratories using reported values of <6.25%, 6.25%, 12.5%, 25%, 50%, 100%, or >100%. If the Median falls between two of these values, then the Assigned Value is set to the higher value.

The Assigned Values for Non-NOEC Analytes are set to the Study Mean, calculated using reported values from 6.25% and 100%, inclusive.

The Lower Acceptance Limit for NOEC Analytes is the test dilution below the Median (or <6.25%, whichever is higher), the Upper Acceptance Limit is the test dilution above the Median (or >100%, whichever is lower). If the Median is between two test dilutions, then the Lower Acceptance Limit is the second test dilution below the Median and the Upper Acceptance Limit is the second test dilution above the Median.

The Acceptance Limits for Non-NOEC Analytes are the Study Mean +/- 2 Standard Deviations. If the upper limit is greater than 100%, then the Upper Acceptance Limit is set to >100%.

If the lower limit is less than 6.25%, then the Lower Acceptance Limit is set to <6.25%.

Not Reported No Value reported.=

Study # : DMRWet-39

2009 TNI Evaluation Checks There are no values reported with < where the assigned value was greater than 0.

2009 TNI Not Acceptable Evaluations Analyte Code Analyte Units Reported Value Assigned Value Acceptance Limits Performance Evaluation Method Description

DMRWet Fathead minnow (Test Code 15) (cat# WET004, lot# Q039-004) 7-day Chronic, Daily Renewal, MHSF Potassium chloride

0808 IC25 (ON) Growth % 19.12 32.9 21.9 - 43.9 Not Acceptable EPA 1000.0 MHSF 2002

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-39

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

Julia Baum Laboratory Manager EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6052

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/16/19

03/22/19 - 07/19/19

DMRWet-39 Laboratory Exception Report

Final Report Results For Laboratory

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-39

Study:

ERA Customer Number:

Laboratory Name:

DMRWet-39

N526160

EcoAnalysts

WET Results

2009 TNI Evaluation Report

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-39

Laboratory Manager EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6052

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/16/19

03/22/19 - 07/19/19

DMRWet-39 Final Report

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Fathead minnow (Test Code 13) (cat# WET002, lot# Q039-002) 48Hr., Acute, Non-Renewal, 25° C, MHSF Potassium chloride

0754 LC50 44.58 45.2 24.7 - 65.6 Acceptable EPA 2000.0 MHSF 25 deg C 5th ED 2002 5/7/2019 -0.0562 45.2 10.2

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Fathead minnow (Test Code 15) (cat# WET004, lot# Q039-004) 7-day Chronic, Daily Renewal, MHSF Potassium chloride

0808 IC25 (ON) Growth 19.12 32.9 21.9 - 43.9 Not Acceptable EPA 1000.0 MHSF 2002 5/7/2019 -2.50 32.9 5.52

0810 NOEC (ON) Growth 12.5 25.0 12.5 - 50.0 Acceptable EPA 1000.0 MHSF 2002 5/7/2019

0756 NOEC Survival 25 25.0 12.5 - 50.0 Acceptable EPA 1000.0 MHSF 2002 5/7/2019

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Ceriodaphnia dubia (Test Code 19) (cat# WET008, lot# Q039-008) 48-Hr Acute, Nonrenewal, 25° C, MHSF Potassium chloride

0764 LC50 34.77 37.0 24.1 - 49.9 Acceptable EPA 2002.0 nonrenewal MHSF 25°C 5th ED 2002 5/8/2019 -0.348 37.0 6.45

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-39

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6052

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/16/19

03/22/19 - 07/19/19

DMRWet-39 Final Report

DMRWet Ceriodaphnia dubia (Test Code 21) (cat# WET010, lot# Q039-010) 3-Brood Chronic, Daily Renewal, MHSF Potassium chloride

0767 IC25 Reproduction 27.48 27.6 20.9 - 34.3 Acceptable EPA 1002.0 MHSF 2002 6/4/2019 -0.0380 27.6 3.33

0768 NOEC Reproduction 25 25.0 12.5 - 50.0 Acceptable EPA 1002.0 MHSF 2002 6/4/2019

0766 NOEC Survival 25 25.0 12.5 - 50.0 Acceptable EPA 1002.0 MHSF 2002 6/4/2019

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Mysid (Test Code 42) (cat# WET016, lot# Q039-016) 48-Hr Acute, Nonrenewal, 25° C, SSW Potassium chloride

0798 LC50 54.75 57.2 28.3 - 86.1 Acceptable EPA 2007.0 Acute 25 deg C 5th ED 2002 5/15/2019 -0.169 57.2 14.4

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Mysid (Test Code 43) (cat# WET017, lot# Q039-017) 7-day Chronic, Daily Renewal, SSW Potassium chloride

0816 IC25 (ON) Growth 28.78 34.7 13.5 - 55.8 Acceptable EPA 1007.0 Chronic 2002 5/15/2019 -0.557 34.7 10.6

0818 NOEC (ON) Growth 25 25.0 12.5 - 50.0 Acceptable EPA 1007.0 Chronic 2002 5/15/2019

0799 NOEC Survival 25 25.0 12.5 - 50.0 Acceptable EPA 1007.0 Chronic 2002 5/15/2019

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Inland silverside (Test Code 44) (cat# WET018, lot# Q039-018) 48-Hr Acute, Nonrenewal, 25° C, SSW Potassium chloride

0803 LC50 68.92 63.8 48.2 - 79.4 Acceptable EPA 2006.0 Menidia Acute 5th

ED 2002 5/23/2019 0.662 63.8 7.80

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-39

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6052

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/16/19

03/22/19 - 07/19/19

DMRWet-39 Final Report

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Inland Silverside (Test Code 45) (cat# WET013, lot# Q039-013) 7-day Chronic, Daily Renewal, SSW Potassium chloride

0825 IC25 (ON) Growth 58.48 57.1 46.1 - 68.1 Acceptable EPA 1006.0 Chronic 2002 5/23/2019 0.252 57.1 5.52

0826 NOEC (ON) Growth 50 50.0 25.0 - 100 Acceptable EPA 1006.0 Chronic 2002 5/23/2019

0824 NOEC Survival 50 50.0 25.0 - 100 Acceptable EPA 1006.0 Chronic 2002 5/23/2019

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Sheepshead minnow (Test Code 46) (cat# WET019, lot# Q039-019) 48-Hr Acute, Nonrenewal, 25° C, SSW Potassium chloride

0804 LC50 35.41 34.6 32.4 - 36.8 Acceptable EPA 2004.0 Acute 5th ED 2002 5/29/2019 0.695 34.6 1.10

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Sheepshead minnow (Test Code 47) (cat# WET020, lot# Q039-020) 7-day Chronic, Daily Renewal, SSW Potassium chloride

0820 IC25 (ON) Growth 52.07 44.1 23.5 - 64.8 Acceptable EPA 1004.0 Chronic 2002 5/29/2019 0.767 44.1 10.3

0822 NOEC (ON) Growth 25 25.0 12.5 - 50.0 Acceptable EPA 1004.0 Chronic 2002 5/29/2019

0805 NOEC Survival 50 50.0 25.0 - 100 Acceptable EPA 1004.0 Chronic 2002 5/29/2019

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-39

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

Brian Hester

P.O. Box 216 Port Gamble, WA 98364

USA

DMR-QA Study

Open Date: 03/23/18

Close Date: 07/06/18

Report Issued Date: 08/03/18

DMRWet-38 Final Report

Study # : DMRWet-38

August 3, 2018

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

Enclosed is your final report for ERA's DMRWet 38 Proficiency Testing study. Your final report includes an evaluation of every result submitted by your facility to ERA.

All analytes in ERA's DMRWet 38 Proficiency Testing study have been evaluated by comparing the reported result to the acceptance limits generated using the criteria contained in the most current TNI FoPT tables.

If you have any "Not Acceptable" evaluations for the DMRWet 38 study, please contact ERA's Customer Service Group at 1-800-372-0122 for your corrective action needs.

Thank you for your participation in ERA's DMRWet 38 Proficiency Testing study. If you have any questions regarding the study, please contact our Proficiency Testing Department at 1-800-372-0122.

Sincerely, Matthew Seebeck Quality Officer attachments

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-38

California WRB (DMRQA) Renee Spears / 916-341-5583 All Analytes 2009 TNI

Louisiana Paul Bergeron / 225-219-3247 All Analytes 2009 TNI

Oregon Stephanie Ringsage / 503-693-4126 All Analytes 2009 TNI

Washington Rebecca Wood / 360-871-8811 All Analytes 2009 TNI

Study # : DMRWet-38

DMRWet-38 Definitions & Study Discussion Study Dates: 03/23/18 - 07/06/18 Report Issued: 08/03/18

DMRWet Study Definitions DMRWet Study Discussion

The Performance Evaluation:

Acceptable

Not Acceptable

No Evaluation

Reported Value falls within the Acceptance Limits.

Reported Value falls outside the Acceptance Limits.

Reported Value cannot be evaluated.

ERA's DMRWet 38 Proficiency Testing study has been reviewed by ERA senior management and certified compliant with the requirements of the 2009 TNI PT Standards and the criteria contained in the current TNI FoPT tables.

ERA's DMRWet 38 study standards were examined for any anomalies. A full review of all homogeneity, stability and accuracy verification data was completed. All analytical verification data for all analytes met the acceptance criteria contained in ththe 2009 TNI PT Standard and the criteria contained in the current TNI Fields of Proficiency Testing (FoPT) tables.

The data submitted by participating laboratories was also examined for study anomalies. There were no anomalies observed during the statistical review of the data.

ERA's DMRWet 38 study reports shall not be reproduced except in their entirety and not without the permission of the participating laboratories. The report must not be used by the participating laboratories to claim product endorsement by any agency of the U. S. government.

The data contained herein are confidential and intended for your use only.

If you have any questions or concerns regarding your assessment in ERA's DMRQA Proficiency Testing program, please contact our Proficiency Testing Department at 1-800- 372-0122.

The Method Description is the method the laboratory reported to ERA.

The Reported Value is the value that the laboratory reported to ERA.

The ERA Assigned Values are compliant with the most current TNI FoPT tables. The Assigned Values are directly traceable to the commercially prepared starting materials used to manufacture the PT standards.

The Acceptance Limits are established per the criteria contained in the most current TNI FoPT tables.

Not Reported No Value reported.=

Study # : DMRWet-38

2009 TNI Not Acceptable Evaluations Analyte Code Analyte Units Reported Value Assigned Value Acceptance Limits Performance Evaluation Method Description

DMRWet Ceriodaphnia dubia (Test Code 21) (cat# WET010, lot# Q038-010) 3-Brood Chronic, Daily Renewal, MHSF Potassium chloride

0767 IC25 Reproduction % 7.56 37.2 19.6 - 54.8 Not Acceptable EPA 1002.0 Chronic 2002

0768 NOEC Reproduction % 6.25 25.0 12.5 - 50.0 Not Acceptable EPA 1002.0 Chronic 2002

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-38 All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

Brian Hester Quality Assurance Director EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/03/18

03/23/18 - 07/06/18

DMRWet-38 Laboratory Exception Report

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-38

Laboratory Name:

DMRWet-38

N526160

EcoAnalysts

WET Results

2009 TNI Evaluation Report

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-38

Quality Assurance Director EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/03/18

03/23/18 - 07/06/18

DMRWet-38 Final Report

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Fathead minnow (Test Code 13) (cat# WET002, lot# Q038-002) 48Hr., Acute, Non-Renewal, 25° C, MHSF Potassium chloride

0754 LC50 65.98 52.2 24.1 - 80.4 Acceptable EPA 2000.0 Acute 5th ED 2002 5/24/2018 0.977 52.2 14.1

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Fathead minnow (Test Code 15) (cat# WET004, lot# Q038-004) 7-day Chronic, Daily Renewal, MHSF Potassium chloride

0808 IC25 (ON) Growth 26.17 42.8 13.4 - 72.3 Acceptable EPA 1000.0 Chronic 2002 5/24/2018 -1.13 42.8 14.7

0810 NOEC (ON) Growth 25 25.0 12.5 - 50.0 Acceptable EPA 1000.0 Chronic 2002 5/24/2018

0756 NOEC Survival 25 25.0 12.5 - 50.0 Acceptable EPA 1000.0 Chronic 2002 5/24/2018

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Ceriodaphnia dubia (Test Code 19) (cat# WET008, lot# Q038-008) 48-Hr Acute, Nonrenewal, 25° C, MHSF Potassium chloride

0764 LC50 63.73 48.4 22.2 - 74.6 Acceptable EPA 2002.0 Acute 5th ED 2002 4/12/2018 1.17 48.4 13.1

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Ceriodaphnia dubia (Test Code 21) (cat# WET010, lot# Q038-010) 3-Brood Chronic, Daily Renewal, MHSF Potassium chloride

0767 IC25 Reproduction 7.56 37.2 19.6 - 54.8 Not Acceptable EPA 1002.0 Chronic 2002 6/5/2018 -3.37 37.2 8.80

0768 NOEC Reproduction 6.25 25.0 12.5 - 50.0 Not Acceptable EPA 1002.0 Chronic 2002 6/5/2018

0766 NOEC Survival 25 50.0 25.0 - 100 Acceptable EPA 1002.0 Chronic 2002 6/5/2018

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-38

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/03/18

03/23/18 - 07/06/18

DMRWet-38 Final Report

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Mysid (Test Code 42) (cat# WET016, lot# Q038-016) 48-Hr Acute, Nonrenewal, 25° C, SSW Potassium chloride

0798 LC50 32.99 41.7 9.93 - 73.5 Acceptable EPA 2007.0 Acute 5th ED 2002 4/6/2018 -0.548 41.7 15.9

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Mysid (Test Code 43) (cat# WET017, lot# Q038-017) 7-day Chronic, Daily Renewal, SSW Potassium chloride

0816 IC25 (ON) Growth 28.97 45.1 21.9 - 68.4 Acceptable EPA 1007.0 Chronic 2002 5/18/2018 -1.39 45.1 11.6

0818 NOEC (ON) Growth 25 25.0 12.5 - 50.0 Acceptable EPA 1007.0 Chronic 2002 5/18/2018

0799 NOEC Survival 25 50.0 25.0 - 100 Acceptable EPA 1007.0 Chronic 2002 5/18/2018

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Inland silverside (Test Code 44) (cat# WET018, lot# Q038-018) 48-Hr Acute, Nonrenewal, 25° C, SSW Potassium chloride

0803 LC50 69.92 60.1 36.9 - 83.3 Acceptable EPA 2006.0 Menidia Acute 5th

ED 2002 5/10/2018 0.846 60.1 11.6

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-38

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/03/18

03/23/18 - 07/06/18

DMRWet-38 Final Report

DMRWet Inland Silverside (Test Code 45) (cat# WET013, lot# Q038-013) 7-day Chronic, Daily Renewal, SSW Potassium chloride

0825 IC25 (ON) Growth 59.5 50.5 31.0 - 70.0 Acceptable EPA 1006.0 Chronic 2002 5/10/2018 0.923 50.5 9.76

0826 NOEC (ON) Growth 50 50.0 25.0 - 100 Acceptable EPA 1006.0 Chronic 2002 5/10/2018

0824 NOEC Survival 50 50.0 25.0 - 100 Acceptable EPA 1006.0 Chronic 2002 5/10/2018

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Sheepshead minnow (Test Code 46) (cat# WET019, lot# Q038-019) 48-Hr Acute, Nonrenewal, 25° C, SSW Potassium chloride

0804 LC50 34.15 35.2 33.0 - 37.5 Acceptable EPA 2004.0 Acute 5th ED 2002 4/6/2018 -0.972 35.2 1.11

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Sheepshead minnow (Test Code 47) (cat# WET020, lot# Q038-020) 7-day Chronic, Daily Renewal, SSW Potassium chloride

0820 IC25 (ON) Growth 55.26 50.7 32.4 - 69.1 Acceptable EPA 1004.0 Chronic 2002 4/13/2018 0.492 50.7 9.18

0822 NOEC (ON) Growth 50 25.0 12.5 - 50.0 Acceptable EPA 1004.0 Chronic 2002 4/13/2018

0805 NOEC Survival 50 50.0 25.0 - 100 Acceptable EPA 1004.0 Chronic 2002 4/13/2018

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-38

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

DMRWet-38 Corrective Action Guidelines

16341 Table Mountain Parkway, Golden, Colorado 80403 800-372-0122 fax (303) 421-0159 www.eraqc.com

Complete ordering and payment information. You will be billed only when your standards ship.3.

We are pleased to accept credit cards. To place your secure order, please call our Waters ERA Customer Service team at 800.372.0122 to provide your credit card information or visit www.eraqc.com to place your order.

Method of Payment:

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Fax this order form to ERA at 303-421-0159. Questions? Call 1-888-372-01224.

Make any necessary changes to Bill To and Ship To addresses.1.

BILL TO: EcoAnalysts

P.O. Box 216

Port Gamble, WA 98364

ATTENTION: Brian Hester

SHIP TO: EcoAnalysts

4770 NE View Dr.

Port Gamble, WA 98364

ATTENTION: Brian Hester

QUANTITY CATALOG

NUMBER

DESCRIPTION UNIT PRICE EXTENDED

PRICE

SHIPPING 35.00

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ESTIMATED TOTAL ORDER

(SALES TAX NOT INCLUDED)

45.00

Make any necessary changes to your corrective action standards below.

Subtotal does not include shipping, handling, and taxes (if applicable).

2.

DMRWet-38 Corrective Action FAX Back Order Form

Customer Number: N526160 DATE: 08/03/2018

P.O. Box 216 Port Gamble, WA 98364

USA

091818L Final Report

Project # :091818L

November 17, 2018

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

Enclosed is your final report for ERA's QuiK™Response program. Your final report includes an evaluation of all results submitted by your laboratory to ERA. None of the assigned value(s) or acceptance limits were available to your laboratory at or before the time of reporting.

Please note the following changes to our final reports:

• At the request of the TNI Accreditation Council, we have included a Laboratory Exception Report that includes a list of all analytes reported with less than qualifiers when the assigned value was greater than “0.” In addition, because we have received many requests from laboratories, this report also includes a list of all analytes with "Not Acceptable" evaluations.

• Some states have elected not to convert to the 2009 TNI Standards at this time. If you have released your results to a state that has retained the 2003 NELAC Evaluation Criteria, your final report will include a section that evaluates the results according to the 2003 Standard in addition to the 2009 TNI Standards.

As part of your accreditation(s), you may be required to identify the root cause of any "Not Acceptable" results, implement the necessary corrective actions, and then satisfy your PT requirements by participating in a supplemental (QuiK™Response) or future ERA PT study. ERA's technical staff is available to help your laboratory resolve any technical issues that may be impairing your PT performance and possibly affecting the quality of your routine data.

The data contained herein are confidential and intended for your use only.

If you are using this report for Corrective Action, please note the following: permittees must submit a copy of this report to your DMR-QA Coordinator, along with your corrective action documentation. Contract Laboratories should send a copy of this report to your permittees upon receipt.

Thank you for your participation in ERA's QuiK™Response program. If you have any questions, please contact our Proficiency Testing Department at 1-800-372-0122.

Sincerely, Matthew Seebeck Quality Officer cc: Project File Number 091818L

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Project # : 091818L

No Recipients Selected

Project # : 091818L

2009 TNI Not Acceptable Evaluations There were no Not Acceptable evaluations for this study.

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Project # : 091818L All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

Brian Hester Quality Assurance Director EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

WA01222

N526160

091818L Laboratory Exception Report

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Project # : 091818L

Project Number:

Laboratory Name:

091818L

N526160

EcoAnalysts

WET Results

2009 TNI Evaluation Report

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Project # : 091818L

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

WA01222

N526160

091818L Final Report

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Ceriodaphnia dubia (Test Code 21) (cat# AQC010, lot# 091818L) Study Dates: 09/18/18 - 11/17/18 (NELAC: Results reported after 45 days.)

3-Brood Chronic, Daily Renewal, MHSF Potassium chloride

0767 IC25 Reproduction 25.4 50.6 24.6 - 76.6 Acceptable EPA 1002.0 Chronic 2002 10/15/2018 -1.94 50.6 13.0

0768 NOEC Reproduction 25 25.0 12.5 - 50.0 Acceptable EPA 1002.0 Chronic 2002 10/15/2018

0766 NOEC Survival 100 50.0 25.0 - 100 Acceptable EPA 1002.0 Chronic 2002 10/15/2018

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Project # : 091818L

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

P.O. Box 216 Port Gamble, WA 98364

USA

DMR-QA Study

Open Date: 03/24/17

Close Date: 07/07/17

Report Issued Date: 08/04/17

DMRWet-37 Final Report

Study # : DMRWet-37

August 4, 2017

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

Enclosed is your final report for ERA's DMRWet 37 Proficiency Testing study. Your final report includes an evaluation of every result submitted by your facility to ERA.

All analytes in ERA's DMRWet 37 Proficiency Testing study have been evaluated by comparing the reported result to the acceptance limits generated using the criteria contained in the most current TNI FoPT tables.

If you have any "Not Acceptable" evaluations for the DMRWet 37 study, please contact ERA's Customer Service Group at 1-800-372-0122 for your corrective action needs.

Thank you for your participation in ERA's DMRWet 37 Proficiency Testing study. If you have any questions regarding the study, please contact our Proficiency Testing Department at 1-800-372-0122.

Sincerely, Patrick Larson Quality Officer attachments

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-37

California WRB (DMRQA) Renee Spears / 916-341-5583 All Analytes 2009 TNI

Louisiana Paul Bergeron / 225-219-3185 All Analytes 2009 TNI

Oregon Chris Redman / 503-693-5706 All Analytes 2009 TNI

Washington Alan Rue / (360) 871-8844 All Analytes 2009 TNI

Study # : DMRWet-37

DMRWet-37 Definitions & Study Discussion Study Dates: 03/24/17 - 07/07/17 Report Issued: 08/04/17

DMRWet Study Definitions DMRWet Study Discussion

The Performance Evaluation:

Acceptable

Not Acceptable

No Evaluation

Reported Value falls within the Acceptance Limits.

Reported Value falls outside the Acceptance Limits.

Reported Value cannot be evaluated.

ERA's DMRWet 37 Proficiency Testing study has been reviewed by ERA senior management and certified compliant with the requirements of the 2009 TNI PT Standards and the criteria contained in the current TNI FoPT tables.

ERA's DMRWet 37 study standards were examined for any anomalies. A full review of all homogeneity, stability and accuracy verification data was completed. All analytical verification data for all analytes met the acceptance criteria contained in ththe 2009 TNI PT Standard and the criteria contained in the current TNI Fields of Proficiency Testing (FoPT) tables.

The data submitted by participating laboratories was also examined for study anomalies. There were no anomalies observed during the statistical review of the data.

ERA's DMRWet 37 study reports shall not be reproduced except in their entirety and not without the permission of the participating laboratories. The report must not be used by the participating laboratories to claim product endorsement by any agency of the U. S. government.

The data contained herein are confidential and intended for your use only.

If you have any questions or concerns regarding your assessment in ERA's DMRQA Proficiency Testing program, please contact our Proficiency Testing Department at 1-800- 372-0122.

The Method Description is the method the laboratory reported to ERA.

The Reported Value is the value that the laboratory reported to ERA.

The ERA Assigned Values are compliant with the most current TNI FoPT tables. The Assigned Values are directly traceable to the commercially prepared starting materials used to manufacture the PT standards.

The Acceptance Limits are established per the criteria contained in the most current TNI FoPT tables.

Not Reported No Value reported.=

Study # : DMRWet-37

2009 TNI Not Acceptable Evaluations There were no Not Acceptable evaluations for this study.

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-37 All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

Brian Hester Quality Assurance Director EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/04/17

03/24/17 - 07/07/17

DMRWet-37 Laboratory Exception Report

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-37

Laboratory Name:

DMRWet-37

N526160

EcoAnalysts

WET Results

2009 TNI Evaluation Report

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-37

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/04/17

03/24/17 - 07/07/17

DMRWet-37 Final Report

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Fathead minnow (Test Code 14) (cat# WET003, lot# Q037-003) 48Hr., Acute, Non-Renewal, 25° C, 20% DMW Potassium chloride

0755 LC50 45.71 42.1 19.7 - 64.5 Acceptable EPA 2000.0 2002 5/10/2017 0.320 42.1 11.2 Julia Baum

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Fathead minnow (Test Code 16) (cat# WET005, lot# Q037-005) 7-day Chronic, Daily Renewal, 20% DMW Potassium chloride

0812 IC25 (ON) Growth 56.98 56.0 39.1 - 72.9 Acceptable EPA 1000 6/21/2017 0.111 56.0 8.45 Julia Baum

0814 NOEC (ON) Growth 50 50.0 25.0 - 100 Acceptable EPA 1000 6/21/2017 Julia Baum

0759 NOEC Survival 50 50.0 25.0 - 100 Acceptable EPA 1000 6/21/2017 Julia Baum

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Ceriodaphnia dubia (Test Code 20) (cat# WET009, lot# Q037-009) 48-Hr Acute, Nonrenewal, 25° C, 20% DMW Potassium chloride

0765 LC50 17.52 17.0 12.6 - 21.3 Acceptable EPA 2002 3/30/2017 0.253 17.0 2.18 Julia Baum

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Ceriodaphnia dubia (Test Code 22) (cat# WET011, lot# Q037-011) 3-Brood Chronic, Daily Renewal, 20% DMW Potassium chloride

0770 IC25 Reproduction 11.04 17.7 < 6.25 - 29.2 Acceptable EPA 1002 6/26/2017 -1.16 17.7 5.75 Julia Baum

0771 NOEC Reproduction 12.5 12.5 6.25 - 25.0 Acceptable EPA 1002 6/26/2017 Julia Baum

0769 NOEC Survival 12.5 12.5 6.25 - 25.0 Acceptable EPA 1002 6/26/2017 Julia Baum

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-37

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

EcoAnalysts P.O. Box 216 Port Gamble, WA 98364

(360) 297-6040-6045

EPA ID:

ERA Customer Number:

Report Issued:

Study Dates:

WA01222

N526160 08/04/17

03/24/17 - 07/07/17

DMRWet-37 Final Report

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Mysid (Test Code 42) (cat# WET016, lot# Q037-016) 48-Hr Acute, Nonrenewal, 25° C, SSW Potassium chloride

0798 LC50 28.56 34.2 27.9 - 40.5 Acceptable EPA 2007 4/20/2017 -1.79 34.2 3.15 Julia Baum

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Mysid (Test Code 43) (cat# WET017, lot# Q037-017) 7-day Chronic, Daily Renewal, SSW Potassium chloride

0816 IC25 (ON) Growth 20.42 29.0 15.5 - 42.5 Acceptable EPA 1007 2002 5/12/2017 -1.27 29.0 6.76 Julia Baum

0818 NOEC (ON) Growth 12.5 25.0 12.5 - 50.0 Acceptable EPA 1007 2002 5/12/2017 Julia Baum

0799 NOEC Survival 12.5 25.0 12.5 - 50.0 Acceptable EPA 1007 2002 5/12/2017 Julia Baum

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Inland silverside (Test Code 44) (cat# WET018, lot# Q037-018) 48-Hr Acute, Nonrenewal, 25° C, SSW Potassium chloride

0803 LC50 73.17 72.4 63.0 - 81.9 Acceptable EPA 2006 4/12/2017 0.159 72.4 4.73 Julia Baum

Analyte Code Test End Point Reported

Value Assigned Value %

Acceptance Limits %

Performance Evaluation Method Description Analysis

Date Z Score Study Mean

Study Standard Deviation

Analyst Name

DMRWet Inland Silverside (Test Code 45) (cat# WET013, lot# Q037-013) 7-day Chronic, Daily Renewal, SSW Potassium chloride

0825 IC25 (ON) Growth 61.2 55.5 38.5 - 72.5 Acceptable EPA 1006.0 2002 6/13/2017 0.674 55.5 8.50 Julia Baum

0826 NOEC (ON) Growth 50 50.0 25.0 - 100 Acceptable EPA 1006.0 2002 6/13/2017 Julia Baum

0824 NOEC Survival 50 50.0 25.0 - 100 Acceptable EPA 1006.0 2002 6/13/2017 Julia Baum

16341 Table Mountain Pkwy • Golden, CO 80403 • 800.372.0122 • 303.431.8454 • fax 303.421.0159 • www.eraqc.com Study # : DMRWet-37

All analytes are included in ERA's A2LA accreditation. Lab Code: 1539-01

ACUTE TOXICITY TESTING: JULY 2019

Prepared for

City of Gig Harbor

Prepared by EcoAnalysts, Inc.

4770 NE View Drive PO Box 216 Port Gamble, WA 98364

NPDES Permit #

EcoAnalysts Report ID

PG072519.01

Submittal Date: August 8, 2019

TOXICITY TESTING RESULTS

Toxicity Testing Results

Report ID: PG072519.01 ii of iv EcoAnalysts, Inc.

All testing reported herein was performed consistent with our laboratory’s quality assurance program.

All results are intended to be considered in their entirety, and EcoAnalysts is not responsible for use of less than the complete report. The test results summarized in this report apply only to the sample(s) evaluated.

APPROVED BY

Author(s):

Acute Toxicity Testing: July 2019

Report ID: PG072519.01 iii of iv EcoAnalysts, Inc.

CONTENTS

1. EXECUTIVE SUMMARY

2. METHODS

2.1 Sample Collection

2.1.1 Sample Receipt Discussion

2.2 Water for Bioassay Testing

2.3 Sample Adjustment

3. RESULTS

3.1 Water Flea (Ceriodaphnia dubia) Acute Test Results

3.2 Fathead Minnow (Pimephales promelas) Acute Test Results

3.3 Quality Assurance/Quality Control

3.4 Data Management and Analysis

4. REFERENCES

TABLES

Table 1-1. Toxicity Test Results Summary Table 1-2. Permit Compliance Results Table 2-1. Biological Testing Performed Table 2-2. Sample Conditions Upon Receipt Table 3-1. Endpoint Summary for the Ceriodaphnia dubia Acute Test Table 3-2. Test Condition Summary for Ceriodaphnia dubia Acute Test Table 3-3. Endpoint Summary for the Pimephales promelas Acute Test Table 3-4. Test Condition Summary for Pimephales promelas Acute Test

Report ID: PG072519.01 iv of iv EcoAnalysts, Inc.

ACRONYMS AND ABBREVIATIONS

ACEC Acute critical effluent concentration

CRFW Cerio Reconstituted Fresh Water

EPA Environmental Protection Agency

LC25 Lethal Concentration to 25% of test population

LC50: Lethal Concentration that results in a 50% reduction in survival

LOEL Lowest Observed Effect Level mg/L Milligrams per liter

NPDES National Pollutant Discharge Elimination System

NOEL No Observed Effect Level

QM Quality manual

RFW Reconstituted Fresh Water

SOP: Standard operation procedure

TU Toxic units

WDOE Washington Department of Ecology

WET Whole Effluent Toxicity

WWTP Waste Water Treatment Plant

Report ID: PG072519.01 1 of 8 EcoAnalysts, Inc.

1. EXECUTIVE SUMMARY

EcoAnalysts conducted Whole Effluent Toxicity (WET) testing on one 24-hour composite effluent sample collected by personnel as part of the effluent characterization. The objective of this program is to assess the potential toxicity of the effluent to aquatic organisms following procedures defined under the facility’s National Pollutant Discharge Elimination System (NPDES) permit.

The results of the toxicity testing are contained in this report.

Statistically significant reduction in survival was not detected at or below the acute critical effluent concentration (ACEC) of 0.73%. The LC50 values were >100% effluent for all endpoints tested in both acute tests. The sample does not exceed the defined permit requirements.

Table 1-1. Toxicity Test Results Summary.

Test Survival in 100% Effluent

NOEL

LOEL

LC25/EC25

LC50/EC50

(%) TU

Ac ut e

Water Flea (Ceriodaphnia dubia) 48-hour Survival 100 100 >100 >100 >100 1

Fathead Minnow (Pimephales promelas) 96-hour Survival

97.5 100 >100 >100 >100 1

NOEL = No Observed Effect Level LOEL = Lowest Observed Effect Level LC25/EC25 = Lethal/Effect Concentration to 25% of test population LC50/EC50 = Lethal/Effect Concentration to 50% of test population TU = 100/NOEL (acute/chronic survival), 100/EC25 (7-day reproduction, growth, and biomass).

Table 1-2. Permit Compliance Results.

Permit Requirement

No defined effluent limit for toxicity testing The Permittee must: Conduct acute toxicity testing on final effluent during November 2018 and July 2019.

The series of five concentrations must include a control, 100% effluent and the ACEC (0.73%)

Result Statistically significant reduction in survival was not detected at or below the acute critical effluent concentration (ACEC) of 0.73% effluent for both tests conducted. Survival in the 100% effluent was above 80% for both tests.

Report ID: PG072519.01 2 of 8 EcoAnalysts, Inc.

2. METHODS

The sample analyzed for toxicity was tested using criteria outlined in the Washington Department of Ecology’s (WDOE) Laboratory Guidance and Whole Effluent Toxicity Test Review Criteria (WDOE WQ-R- 95-80 (2016)). These criteria are further defined through the Environmental Protection Agency’s (EPA) most recently promulgated effluent guidance documents outlined in Section 4.

Bioassay testing for this project consisted of two acute bioassays. The tests conducted in support of this project are summarized in Table 2-1.

Table 2-1. Biological Testing Performed.

Test Type Test Descriptor Species Method

Acute

48-hour Survival Ceriodaphnia dubia

Water Flea

WDOE WQ-R-95-80 (2016); EPA-

821-R-02-012 Method 2002.0;

SOP TOX004.09

96-hour Survival Pimephales promelas

Fathead Minnow

WDOE WQ-R-95-80 (2016); EPA-

821-R-02-012 Method 2000.0;

SOP TOX017.08

2.1 Sample Collection

personnel collected one effluent sample on July 16, 2019 at 0600 hours. The sample was transported to the EcoAnalysts Port Gamble laboratory by Gig Harbor WWTP personnel and received on the same day as collection. Sample temperature upon receipt was 4.4°C.

Table 2-2. Sample Conditions Upon Receipt.

Sample COGH Plant Effluent Comp.

Laboratory ID P190716.01

Date/Time Sampled 7/16/19; 0600

Date/Time Received 7/16/19; 0850

Time Between Sampling and Receipt 2 hours, 50 minutes

Dissolved Oxygen (mg/L) Recommended: >4.0 mg/L

10.4

Temperature (°C) Upon Receipt Ideal: 4°C or <1 hour from sample: 0 – 20°C <4 hours from sample: 0 – 12°C ≥4 hours from sample: 0 – 6°C

4.4 pH (units) Recommended: 6 – 9

7.2

Conductivity (µS/cm) 484

Hardness (mg/L CaCO3) 64

Alkalinity (mg/L CaCO3) 97

Total Chlorine (mg/L) 0.05

Total Ammonia (mg/L) 0.00

Report ID: PG072519.01 3 of 8 EcoAnalysts, Inc.

2.1.1 Sample Receipt Discussion

Sample temperature upon receipt was within the recommended temperature range of 0 - 6°C.

Additional sample conditions are summarized in Table 2-2. The effluent sample was held in a walk-in cold room at 4 ± 2 ºC in the dark until utilized for testing.

2.2 Water for Bioassay Testing

Freshwater diluent used in this study was prepared using the EPA method for standard, synthetic moderately hard, reconstituted water, using the reagent grade chemicals (USEPA 2002a). Extensive testing on a variety of test species has shown that there is no significant potential for toxicity or bioaccumulation of contaminants from this water supply. Chemical analysis of this water source is conducted and reviewed on an annual basis.

2.3 Sample Adjustment

The samples did not require adjustments prior to analysis.

3. RESULTS

The results of the effluent testing are presented in this section. Statistical comparisons and laboratory documents and are provided in Appendix A. Chain-of-custody and sample receipt logs are provided in Appendix B.

3.1 Water Flea (Ceriodaphnia dubia) Acute Test Results

The acute toxicity test with C. dubia was initiated on July 17, 2019. The test met the control acceptability criteria of ≥90% mean survival with a mean control survival of 95%.

Mean survival and statistical results are summarized in Table 3-1. The test conditions are summarized in Table 3-2.

Concentrations of 0.73, 3, 20, 50, and 100% effluent were prepared utilizing laboratory water. This concentration series includes the acute critical effluent concentration (ACEC) of 0.73%.

Water quality parameters were within the acceptable limits throughout the duration of the 48-hour static test.

The LC50 for the ammonia reference-toxicant test was 41.2 mg/L total ammonia and was within two…