4-Appendix II - 2024-2025 Fecal Coliform Analysis.pdf

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1591 East Canal Road House Demo
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York County, Pennsylvania

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This is a fecal coliform analysis report for biosolids testing conducted by the Pennsylvania Department of Environmental Protection (PA DEP) Laboratory for Dover Township's Wastewater Treatment Facility, covering the 2024-2025 fiscal years. The document presents quarterly fecal coliform testing results measured in Most Probable Number per gram (MPN/gm) using EPA Method 1680, with all analyses performed under PA DEP Lab ID #22-00293. Testing was conducted across four quarters in both 2024 and 2025, with samples collected on the following dates: February 27, 2024; May 23, 2024; August 22, 2024; October 17, 2024; March 19, 2025; May 28, 2025; August 11, 2025; and December 10, 2025. Each quarterly sampling event included seven individual measurements to establish geometric means and standard deviations for biosolids quality assessment.

The regulatory benchmark for Class B biosolids establishes a fecal coliform limit of 2,000,000 CFU/gram as a geometric mean. The 2024 results demonstrated compliance with all quarterly geometric means ranging from 42,312 MPN/gm (first quarter) to 159,048 MPN/gm (fourth quarter), well below the regulatory threshold. The 2025 data similarly remained compliant, with geometric means ranging from 65,679 MPN/gm (first quarter) to 220,012 MPN/gm (fourth quarter). Variability in results is reflected in standard deviations, with 2025 showing higher variability in the first quarter (123,163) and fourth quarter (141,551) compared to other periods. This testing data supports the biosolids disposal operations outlined in Dover Township's five-year biosolids disposal contract solicitation scheduled for bid opening on April 9, 2026, which addresses the disposal of approximately 3,500 wet tons annually of aerobically digested biosolids from the treatment facility.

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FECAL COLIFORM TESTING 2024

BIOSOLIDS

(LIMIT FOR CLASS B = 2,000,000 CFU/GRAM as geometric mean)

1st Quarter 2nd Quarter DATE MPN/gm DATE MPN/gram

2/27/2024 43,400 5/23/2024 13,400 2/27/2024 37,400 5/23/2024 22,700 2/27/2024 44,400 5/23/2024 65,400 2/27/2024 42,600 5/23/2024 16,500 2/27/2024 44,000 5/23/2024 83,100 2/27/2024 41,900 5/23/2024 12,000 2/27/2024 42,900 5/23/2024 65,800 geometric mean 42,312 geometric mean 29,934 std deviation 2,349 std deviation 30,308

3rd Quarter 4th Quarter DATE MPN/gm DATE MPN/gm

8/22/2024 55,900 10/17/2024 120,000 8/22/2024 88,800 10/17/2024 280,000 8/22/2024 69,300 10/17/2024 133,000 8/22/2024 88,500 10/17/2024 123,000 8/22/2024 67,600 10/17/2024 185,000 8/22/2024 172,000 10/17/2024 274,000 8/22/2024 73,100 10/17/2024 92,400 geometric mean 82,438 geometric mean 159,048 std deviation 38,891 std deviation 76,591

Analyses done by PA DEP Lab ID #22-00293 Method EPA 1680 ("MPN")

FECAL COLIFORM TESTING 2025

BIOSOLIDS

(LIMIT FOR CLASS B = 2,000,000 CFU/GRAM as geometric mean)

1st Quarter 2nd Quarter DATE MPN/gm DATE MPN/gram

3/19/2025 67,400 5/28/2025 57,100 3/19/2025 9,150 5/28/2025 180,000 3/19/2025 108,000 5/28/2025 88,700 3/19/2025 56,200 5/28/2025 67,900 3/19/2025 22,700 5/28/2025 89,300 3/19/2025 170,000 5/28/2025 67,400 3/19/2025 365,000 5/28/2025 41,700 geometric mean 65,679 geometric mean 76,644 std deviation 123,163 std deviation 45,298

3rd Quarter 4th Quarter DATE MPN/gm DATE MPN/gm

8/11/2025 173,000 12/10/2025 48,000 8/11/2025 90,700 12/10/2025 418,000 8/11/2025 70,100 12/10/2025 460,000 8/11/2025 56,400 12/10/2025 267,000 8/11/2025 90,000 12/10/2025 191,000 8/11/2025 68,300 12/10/2025 188,000 8/11/2025 92,900 12/10/2025 282,000 geometric mean 86,222 geometric mean 220,012 std deviation 38,460 std deviation 141,551

Analyses done by PA DEP Lab ID #22-00293 Method EPA 1680 ("MPN")

2024 Fecal Coliform Analysis.pdf
fecal coliform 2024
2025 Fecal Coliform Analysis.pdf
fecal coliform 2025

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