ADDEN2~4.PDF

ATP Bacterial Clearance Assessment

San Bernardino County- Department of Public Health 451 E. Vanderbilt Way

San Bernardino, CA 92408

Prepared for:

Servpro South Redlands by Jeffrey Padgett

C/O Allison Padgett

Prepared by:

Charles Taylor Environmental Technical Services

301 Merritt 7 2nd Floor

Norwalk, CT 06851 Email:enviro@charlestaylor.com

Date:

04/18/2025

Project, Claim #:

2128561, PO 592515

mailto:enviro@charlestaylor.com

1 Introduction Charles Taylor Environmental (CTE) was retained to conduct an Adenosine Triphosphate (ATP) Microbial Assessment of the property referenced above. The survey and sampling were conducted by Jared Paderez.

2 Field Survey and Analytical Methodology The purpose of the ATP testing was to ascertain the effectiveness of cleaning activities in response to a water intrusion loss which potentially contaminated the surfaces in the area of concern with bacteria containing water.

The scope of services for the sampling included the following tasks:

• Conducted a visual survey of the identified scope of work area/containment(s) to identify overall cleanliness.

• Assess the moisture content of any remaining building materials such as walls, framing, or other materials remaining in the area(s). Representative surface areas of each impacted finishing material(s) were tested with a moisture meter.

• Visually assess the cleaning and control methods used onsite such as High Efficiency Particulate Air (HEPA) filtration, wood grinding, and/or chemical application, if applicable.

• Collected representative swab samples of surfaces within the affected area(s) using Hygiena® UltraSnapTM ATP Surface Swabs.

• Analyzed collected swabs using the Hygiena® EnSURETM Touch Monitoring System.

• Recorded results onto a chain of custody for future reference and reporting.

• Reported findings of results to the client/representative for additional restoration, if unacceptable, or for notification that reconstruction can begin, if results are acceptable.

• Collected photos of the work area(s) and materials. (Available upon request).

2.1 Sample Analysis and Results

The evaluation of the data used for this bacterial clearance assessment was obtained by using the Hygiena® EnSURETM Touch Monitoring system. Hygiena® EnSURETM Touch Monitoring system is centered upon the measurement of ATP – the energy molecule for all animal, vegetable, bacteria (sewage related bacteria), yeast and mold cells. ATP is an indicator of organic residues and microorganisms. Detection of ATP is commonly used to rapidly assess the standards of hygiene and sanitation for surfaces, such as in the healthcare or food preparation industry. The Hygiena® EnSURETM Touch Monitoring system is a handheld electronic device that is used to measure the amount of ATP from the surface swab sample. The ATP surface swab test is a single-use apparatus that contains a swab for the collection of a sample from a 100 cm2 surface.

The swabs are each individually packaged and are each pre-moistened with a cationic agent to aid in the collection of surface residue and the release of ATP from intact cells.

Upon activation of the test within the swab capsule, a reagent in the cuvette reacts with ATP collected on the swab to produce light. The intensity of the light is proportional to the amount of ATP present within the sample and therefore, the degree of contamination. Measurement of the light requires the use of the luminometer, and the results are displayed in Relative Light Units (RLU), which are directly compared to pre-determined “band” acceptable/unacceptable levels. The EnSure™ Touch Monitoring System is calibrated prior to each use by positive and negative standard calibration swabs.

Clearance criteria for post cleaning or disinfecting are as follows:

Table 1: Default Clearance Criteria

Results interpretation was provided by Hygiena® data literature and interpreted as they would apply to current Institute of Inspection, Cleaning, and Restoration Certification (IICRC®) S500 and S520 protocols. These guidelines are for general use. Unique non-porous and semi-porous matrices may require on-site calibration, where an acceptable reading is achieved by averaging 5-10 readings from known non-impacted and/or adequately treated areas. Especially in larger facilities with multiple combinations or ranges of risk factors, such as direct contact of the surface (contact with a non-porous surface with bare hands or food) versus indirect contact (foot traffic, walking on a semi-porous masonry floor with shoes) also can influence stringency of acceptability in the best judgement of the inspector after recalibration readings.

EnSure Sampling Result Result Interpretation Notes

<50 RLU Acceptable Residential or Medical Building. No additional cleaning or testing required.

<100 RLU Acceptable Commercial or Public Building. No additional testing required.

<20 RLU Acceptable Food Preparation or Medical

Procedural Room. No additional testing required.

3 Conclusions and Recommendations Based on visual observations made during the survey and ATP analysis results, the following conclusions and recommendations are provided:

All analytical results within the acceptable clearance range are considered completed. The area(s) listed above are ready for new or continued construction where an “Acceptable” result was achieved. Prior to new or continued construction, additional cleaning, decontamination, or applicable restoration should be performed in the area(s) where an “Unacceptable” level was achieved.

4 Limitations This survey was conducted in conformance with best practices from the Hygiena®, IICRC® S500 and S520, and laboratory-specific sample collection protocols. Charles Taylor Environmental utilizes established best practices and techniques in accordance with industry standards while performing this survey. Charles Taylor Environmental cannot be responsible for changing conditions that may alter relative exposure risk or for future changes in accepted methodology.

Charles Taylor Environmental does not guarantee either expressed or implied that all microbes or bacteria(s) were sampled during this survey. Charles Taylor Environmental was retained only to perform the assessment within the scope of work area(s). The findings shall only be applicable to the representative samples taken, the exact sample locations, at the time that the samples were collected. In some cases, inaccessible, hidden or indistinguishable materials may not have been sampled. In all cases, Charles Taylor Environmental evaluates and tests surface materials and makes recommendations based on findings in accessible areas at the time of the assessment. Charles Taylor Environmental is not in any way responsible or liable to locate any contamination or material damage within inaccessible or hidden areas. If additional suspect contaminated or damaged materials are located during any subsequent remediation, construction, or selective demolition, Charles Taylor Environmental can evaluate those materials as a wholly distinct and additional scope of work project than this evaluation herein. Finally, Charles Taylor Environmental shall not be held responsible for the deficiencies or omissions of others in relation to the services contracted herein.

Sample ID Location Description

EnSure Sampling

Result

(RLU)

Result Interpretation

(Acceptable/Non- Acceptable)

S01 Office 1 Floor – Concrete 3 Acceptable S02 Office 1 Framing – Metal 0 Acceptable S03 Office 2 Floor – Concrete 2 Acceptable S04 Office 2 Framing – Metal 0 Acceptable S05 Outside Bathroom Floor – Concrete 0 Acceptable S06 Outside Bathroom Framing – Metal 11 Acceptable S07 Bathroom Floor – Tile 14 Acceptable

5 References https://www.hygiena.com/ https://iicrc.org/

Signature and Consultant Certification

Stephanie Davis Report prepared by:

Jared Paderez Inspector Name

Michael Nave Senior Reviewer Name

Attachments:

Onsite Chain-of-Custody https://www.hygiena.com/ https://iicrc.org/ https://v3.camscanner.com/user/download