05-ASI - Final Data Report - Raritan River PART 1of4 - 12 May.pdf

VOLUME I

Technical Report on the Sampling and Testing of Material from the Raritan River

Proposed for Dredging and HARS Ocean Disposal

Contract No. W912DS-17-D-0006 Delivery Order No. 0054

Prepared for

Army Corps of Engineers, New York District 26 Federal Plaza

New York, NY 10278

February 22, 2019

ASI Job # 38-062

TABLE OF CONTENTS

VOLUME I, Executive Summary

Signature Page Summary Page I. Introduction A. Project Overview

B. Relevant Correspondence C. Project Deviations Summary

II. Test Administration A. Sponsor B. Testing Facilities C. Dates of Experiment D. Study Participants

III. Materials and Methods A. Sampling B. Homogenizing and Compositing C. Chemical Analyses D. Physical Analysis and Total Organic Carbon E. Solid Phase Testing F. Suspended Particulate Phase Testing G. Bioaccumulation Testing

IV. Results and Discussion A. Solid Phase Testing B. Suspended Particulate Phase Testing C. Bioaccumulation Testing

V. References

FIGURES AND TABLES

Figure 1 Site Map Table 1 Sample Identification and Compositing Table 2 DGPS Coordinates, Core Locations Table 3 Test Performance Summary (% Survival) Table 4a Particle Size Distribution, Percent Moisture and TOC Values of

Test Sediments Table 4b Particle Size Distribution, Percent Moisture and TOC Values of

Reference and Control Sediments Table 5 Specific Gravity, Bulk Density, and Atterberg Limits Table 6 Sampling Summary and Holding Times

Water Quality Parameter Tables

Solid Phase A. abdita Static Non-Renewal Bioassay

Table 7 Live Count Table 8 Temperature Table 9 Salinity Table 10 D.O Table 11 pH Table 12 Total Ammonia

Solid Phase A. bahia Static Non-Renewal Bioassay

Table 13 Live Count Table 14 Temperature Table 15 Salinity Table 16 D.O Table 17 pH Table 18 Total Ammonia Table 19 Unionized Ammonia

Suspended Particulate Phase M. beryllina Bioassay

Table 20 Live Count Table 21 Temperature Table 22 Salinity Table 23 D.O Table 24 pH Table 25 Total Ammonia

Suspended Particulate Phase A. bahia Bioassay

Table 26 Live Count Table 27 Temperature Table 28 Salinity Table 29 D.O Table 30 pH Table 31 Total Ammonia

Suspended Particulate Phase M. galloprovincialis Bioassay

Table 32 LC50 for Survival Table 33 EC50 for Development

Bioaccumulation M. nasuta Bioassay Table 34 Live Count Table 35 Temperature Table 36 Salinity Table 37 D.O Table 38 pH

Bioaccumulation N. virens Bioassay

Table 39 Live Count Table 40 Temperature Table 41 Salinity Table 42 D.O Table 43 pH

VOLUME II, APPENDICES A – H

Appendix A: Sediment Core Logs Appendix B: Photographs Appendix C: Chains of Custody Appendix D: Sample Use Forms Appendix E: Sea Water Sample Receiving Forms & Chemical Analyses Appendix F: Percent Moisture & Grain Size Distribution Raw Data Appendix G: Bulk Density, Specific Gravity, and Atterberg Limits Appendix H: Total Organic Carbon Raw Data

VOLUME III, APPENDICES I – N

Appendix I: Solid Phase A. abdita Static Non-Renewal Bioassay Bench sheets Control Chart Standard Reference Toxicant Data Organism Transportation Sheets Continuous Temperature Recorder

Appendix J: Solid Phase A. bahia Static Non-Renewal Bioassay Bench sheets, Static Non-Renewal Control Chart Standard Reference Toxicant Data Organism Transportation Sheets Continuous Temperature Recorder

Appendix K: Suspended Particulate Phase M. beryllina Bioassay Bench Sheets

Statistical Analysis, LC50 Calculations Control Chart Standard Reference Toxicant Data Organism Transportation Sheets Continuous Temperature Recorder

Appendix L: Suspended Particulate Phase A. bahia Bioassay

Bench sheets Statistical Analysis, LC50 Calculations Control Chart Standard Reference Toxicant Data Organism Transportation Sheets Continuous Temperature Recorder

Appendix M: Suspended Particulate Phase M. galloprovincialis Bioassay Bench sheets Statistical Analysis, LC50/EC50 Calculations Control Chart Standard Reference Toxicant Data Organism Transportation Sheets Continuous Temperature Recorder

Appendix N: EcoAnalysts- Port Jersey Sediment Bioaccumulation Toxicity Evaluation Report and Raw Data

VOLUME IV, Public Notice Tables

Table 1 Chemical Analysis of Site Water and Elutriate Table 2 Toxicity Test Results Table 3 Chemical Analysis of Tissue Supermemo

VOLUME V, Statistical Analysis of Tissue Samples: M. nasuta

VOLUME VI, Statistical Analysis of Tissue Samples: N. virens

Summary Page

Technical Report on the Sampling and Testing of Material from the Raritan River

Proposed for Dredging and HARS Ocean Disposal

Contract No. W912DS-17-D-006 Delivery Order No. 0054

Study Initiation Date

May 14, 2018

Study Completion Date

February 22, 2019

Performing Laboratory

Aqua Survey, Inc.

469 Point Breeze Road

Flemington, New Jersey 08822

Sponsor

Army Corps of Engineers, New York District

Laboratory Project ID

38-062

I. INTRODUCTION

A. Project Overview

The objective of this study was to perform sampling, compositing, physical characterization and biological testing of sediment proposed for dredging from the Raritan River. Testing was performed in accordance with USEPA and USACOE, 1991 Green Book, Evaluation of Dredged Material Proposed for Ocean Disposal – Testing Manual, EPA-503/8- 91/001; and USACOE New York District EPA Region 2, Guidance for Performing Tests on Dredged Material Proposed for Ocean Disposal, April 2016.

Aqua Survey, Inc. (ASI) performed all sampling, physical analyses (grain size, percent moisture, TOC, bulk density, specific gravity and Atterberg Limits) and the Suspended Particulate and Solid phases of the biological testing. EcoAnalysts, Inc. performed the Bioaccumulation phase of the biological testing.

Whole sediment toxicity was assessed through 10-day exposures with the mysid shrimp, Americamysis bahia, and the amphipod, Ampelisca abdita in solid phase tests.

Toxicity of elutriate prepared from the composite was assessed through 96-hour suspended particulate phase toxicity bioassays with the inland silverside, Menidia beryllina and the mysid shrimp, Americamysis bahia.

A 48-hour embryo development test was performed using the blue mussel, Mytilus galloprovincialis.

Bioaccumulation of metals, pesticides, PCB congeners, semivolatiles (1,4 dichlorobenzene), PAHs, and dioxin/furans was assessed using 28-day exposures of the clam, Macoma nasuta and the sand worm, Nereis virens to the composite sediment samples.

B. Relevant Correspondence

The following pages provide relevant correspondence for this project.

C. Project Deviations Summary

There were no deviations from the project plan.

II. TEST ADMINISTRATION

A. Sponsor

Army Corps of Engineers, New York District (NYD/COE)

B. Testing Facilities

ALS Global Kelso Environmental Laboratory 1317 S. 13th Avenue Kelso, WA 98626

Aqua Survey, Inc.

469 Point Breeze Road Flemington, NJ 08822

Battelle 141 Longwater Drive, Suite 202 Norwell, MA 02061

Battelle 505 King Avenue Columbus, OH 43201-2693

EcoAnalysts, Inc.

4770 NE View Drive Port Gamble, WA 98364

C. Dates of Experiment

Date of Study Initiation: May 14, 2018 Date of Study Completion: February 22, 2019

D. Study Participants

Jon Doi, Ph.D. Executive Vice President Thomas Dolce Field Operations Manager Cheryl Hall Quality Assurance Officer Elizabeth Horn Staff Scientist

Kevin Sondag Field Operations Specialist Robert Fristrom Field Operations Support Michelle Thomas Laboratory Manager

III. MATERIALS AND METHODS

All sampling and testing was performed according to the sediment sampling and testing plan on file with the NYD/COE.

A. Sampling

Test sediment from 14 locations and site water were collected from the Raritan River on May 14-17 and May 21, 2018. The project depth was -27 feet MLLW including 2 ft overdredge allowance. Figure 1 provides the site map.

The vessel used for sampling, The R/V Schuylkill, was positioned using a Trimble SPS 855 Differential Global Positioning System (DGPS).

Collection of sediment was performed using a Rossfelder P-3 vibracore with flexible plastic core liners. Every core taken was inspected and its characteristics were recorded on a sediment core log. Photographs were taken of each core and are provided in Volume II, Appendix B.

Upon arrival at ASI, all samples were logged in and assigned a unique sample number (Table 1). Samples were received at ASI in Flemington, NJ under chain of custody and stored at 2◦C to 4◦C until testing. All supporting documentation, including chains-of-custody and sample use forms, can be found in Volume II (Appendices A-H) and Volume III (Appendices I-N).

There were four control sediment samples and one reference sediment used in the biological bioassays. The A. abdita control (ASI ID 20180368) was obtained from Tuckerton Creek at the third bridge, Tuckerton, NJ, on June 6, 2018. The A. bahia control sediment (ASI ID 20180359) was obtained from the Gateway National Recreation Area, Atlantic Highlands, NJ, on May 29, 2018. The M. nasuta control sediment (ASI ID 20180902), provided by the organism supplier was obtained from Discovery Bay, Washington and the N. virens control sediment (ASI ID 20180901), also provided from the organism supplier, was obtained from the Damariscotta River, Boothbay Harbor, ME. The Mud Dump Reference sediment (ASI ID 20180370) was obtained from the ACOE-designated area six miles off of the coast of Sandy Hook, NJ on June 20, 2018 (sampling coordinates: 40 20.193 N, 73 52.196 E).

Prior to the initiation of testing, all control, reference, and test sediments were sieved using a 2-mm mesh screen. For the A. abdita solid phase test, the reference and test sediment were sieved through a 1-mm mesh screen.

The control sediment was sieved through a 0.5 mm mesh screen.

Manasquan Water, obtained from Manasquan Inlet, NJ, was used as the overlay water and dilution water for the suspended particulate and solid phase testing.

Seawater, obtained from the Northern Hood Canal at Port Gamble, WA, was used as the overlay water for the bioaccumulation testing.

These waters were tested according to ASTM guidelines to ensure purity.

The results of the latest water analysis is included in Volume II (Appendix E).

B. Homogenizing and Compositing

Each sediment sample was carefully homogenized using a stainless steel mixer following specific guidelines found on pages 9-11 and Appendix A of the Dredging Manual and according to ASI’s standard operating procedure SOP/PRP/008. Samples were mixed until uniform in color and texture. These homogenized core samples were then combined using the same methodology to form two composite samples. Sample identification numbers and the compositing scheme are provided in Table 1.

An 800 ml subsample from each composite was archived, as required by the Corps, for possible future analysis.

C. Chemical Analyses

Subsamples of the composites were shipped to Battelle, Norwell, MA, and ALS Global, Kelso Environmental Laboratory, WA, along with the site water sample for chemical analysis as required by the NYD/COE.

Following chain-of-custody procedures, samples were placed in glass jars and shipped by overnight courier in coolers with ice packs.

D. Physical Analysis and Total Organic Carbon

Sub-samples of all sediments were analyzed by ASI personnel for physical parameters. These sediments include the following: each homogenized core, the two composites, the Mud Dump Reference sediment, and the four Control sediments. Percent moisture and grain size distribution analyses were performed in accordance with the Standard Test Method for Particle-Size Analysis of Soils, Designation: D422-63, Re-approved 2002

[ASTM, 2002].

Total Organic Carbon (TOC) was determined based on the guidance from EPA Office of Solids Waste and Emergency Response SW-846 Method No. 9060 (Volume IC, Chapter 5, Revision 0, 9/86) [EPA, 1986]. The instrument used for this analysis was the Dohrmann TOC Boat Sampler, Model 183 (serial number 98202003), which was connected to the Dohrmann Apollo 9000 TOC Analyzer.

Additionally, ASI analyzed the composite samples for Bulk Density [Blake, 1986], Specific Gravity [APHA, 1995] and Atterberg Limits (plastic and liquid limits) [ASTM, 1995]. Results of the percent moisture, grain size distribution and TOC analyses are provided in Tables 4a and 4b.

Results of the bulk density, specific gravity, and Atterberg limits are provided in Table 5.

E. Solid Phase Testing

Ampelisca abdita, Americamysis bahia

Whole sediment toxicity of the composites was assessed through 10-day exposures with the amphipod, A. abdita, and Mysid shrimp, A. bahia, in solid phase bioassays [ASTM, 1999].

The initial pore water ammonia readings for the composites were 142 mg/L for Reach A and 71.1 mg/L for Reach B, which exceeds the

20.0 mg/L threshold established by Davies et al, [Davies, 1993]. Due to this, pretest purging of the sediments for both of the solid phase studies (A.

abdita and A. bahia) was initiated as per the ammonia reduction procedure outlined in the RTM (USACE/USEPA 2016), Section 6.4.5.

After 8-10 days of pretest purging for Reach A (8 days for A. abdita and 10 days for A. bahia) and 6-8 days of pretest purging for Reach B (6 days for A. abdita and 8 days for A, bahia), the ammonia levels were measured to be below the required 20.0 mg/L threshold. Therefore, chambers were set out and testing was initiated the following day.

The 10-day studies were conducted as static non- renewal and followed the recommended procedures outlined in the RTM (USACE/USEPA 2016).

Final live count and water quality parameter tables can be found at the end of this report.

Standard reference toxicant tests were performed for both organisms, A. bahia and A. abdita. The LC50 values fell within the 95% confidence limit of their respective control charts. The control chart can be found in Volume III (Appendix J).

The A. abdita used in testing were obtained from Aquatic Research Organisms, Hampton, NH. Organisms were held for 4 days prior to testing; during this time they were acclimated to test temperature and salinity.

The A. bahia used in testing were 5-day-old juveniles and were obtained from Aquatic Research Organisms, Inc., Hampton, NH.

Organism receiving and holding forms can be found in Volume III, Appendices I and J.

F. Suspended Particulate Phase Testing

Menidia beryllina, Americamysis bahia and Mytilus galloprovincialis

Toxicity of elutriates prepared from the composite was assessed through 48- and 96-hour suspended particulate phase toxicity tests using three test species, the inland silverside, M. beryllina, the Mysid shrimp, A. bahia, and the mussel, M. galloprovincialis.

The M. beryllina used for testing were 12 days old and were obtained from Aquatic Research Organisms, Hampton, NH.

The A. bahia used for testing were 4-day-old juveniles obtained from Aquatic Research Organisms, Hampton, NH.

The M. galloprovincialis used for testing were fertile adults of various ages and were obtained from Carlsbad Aquafarms, Carlsbad, CA.

Organism receiving and holding forms can be found in Volume III, Appendices K, L, and M.

The SPP studies were conducted following the recommended procedures outlined in the RTM (USACE/USEPA 2016).

After 96 hours of exposure to elutriate concentrates, final live count data were transferred into a spreadsheet, sorted and tabulated for M. beryllina and A. bahia.

After 48 hours of exposure to the elutriate concentration, an EC50 for development and an LC50 for survival were calculated using ToxCalc™ software when one or more concentrations showed at least a 50% effect on the M. galloprovincialis larvae.

Standard reference toxicant tests were performed for M. beryllina, A. bahia, and M. galloprovincialis as cited in the work plan. Reference toxicant data were entered into ToxCalc™ and an LC50 was calculated.

The LC50 for all three species fell within the 95% confidence limits of their respective control charts. Control charts for each species as well as all supporting bioassay documentation can be found in Volume III, Appendices K, L, and M.

G. Bioaccumulation Testing

Macoma nasuta, Neanthes (formerly Neries) virens

The bioaccumulation of metals, pesticides, PCBs, semivolatiles, (1-4 dichlorobenzene), PAHs and dioxin/furans from the composites were assessed using 28-day exposures of the clam, M. nasuta and the sand worm, N. virens. The Bioaccumulation studies were conducted following the recommended procedures outlined in the RTM (USACE/USEPA 2016).

The M. nasuta used in testing were adults of various ages and were obtained from J & G Gunstone, Discovery Bay, WA.

The N. virens used for testing were adults of various sizes and were obtained from Aquatic Research Organisms, Hampton, NH.

Organism receiving and holding forms can be found in Volume III, Appendix N.

After 28-days of exposure to the composites, organisms were depurated overnight in clean seawater. Tissue from each exposure chamber was placed in individual glass jars, weighed and frozen before sending to Battelle by overnight courier for chemical analysis. Tissue samples were archived by Battelle.

Standard reference toxicant tests were performed for both species as cited in the work plan. Water quality and physical parameters were also monitored as per the work plan. The LC50 values for both species fell within the 95% confidence limits of their respective control charts. A control chart for each species, as well as all supporting bioassay documentation data can be found in Volume III, Appendix N.

IV. RESULTS AND DISCUSSION

Test results of all the bioassays as well as water quality parameters are summarized in the tables at the end of this volume. Raw data for physical characteristics, biological effects and water quality parameters, are presented in Volume III, Appendix N.

A. Solid Phase Testing

Ampelisca abdita

After 10 days, survival of A. abdita organisms exposed to the composites was as follows:

A. abdita Survival

Control Reference Reach A Reach B

100% 99% 100% 96%

In accordance with the 1991 Federal Guidance [USEPA, 1991], samples are considered toxic if survival of animals exposed to test sediment are at least 20% less than the survival observed in the reference sediment and this difference is statistically significant (p = 0.05) after a ten-day test period.

These results indicate that the composites are not acutely toxic to A.

abdita.

Americamysis bahia

After 10 days, the survival A. bahia exposed to the sediments did not show a statistically significant reduction in survival when compared to the organisms in the reference sediment. The unionized ammonia level in the test sediment was < 0.6 mg/L.

After 10 days, survival of A. bahia organisms exposed to the composites was as follows:

A. bahia Survival

100% 98% 98% 96%

In accordance with the 1991 Federal Guidance [USEPA, 1991], samples are considered toxic, if survival of animals exposed to test sediment are at least 10% less than the survival observed in the reference sediment and this difference is statistically significant (p = 0.05) after a ten-day test period.

These results indicate that the composites are not acutely toxic to A. bahia.

B. Suspended Particulate Phase Testing

Menidia beryllina

After 96 hours, M. beryllina exposed to the suspended particulate phase elutriates resulted in an LC50 values of 52.0% for Reach A and 81.0% for Reach B.

M. beryllina Survival

Concentration Reach A Reach B

Control 94%

10% 97% 98%

50% 70% 95%

100% 0% 27%

Americamysis bahia

After 96 hours, A. bahia exposed to the suspended particulate phase elutriates resulted in an LC50 values of 65.0% for Reach A and >100% for Reach B.

A. bahia Survival

Control 90%

10% 97% 92%

50% 85% 90%

100% 1% 84%

Mytilus galloprovincialis

After 48 hours, M. galloprovincialis larvae exposed to the suspended particulate phase elutriates resulted in EC50 values for development of 22.0% for Reach A and 22.3% for Reach B. The LC50 values for survival were 60.0% for Reach A and >100% for Reach B.

M. galloprovincialis Survival

Control 97.1%

10% 88.8% 96.3%

50% 72.0% 69.4%

100% 11.9% 63.9%

Control survival was greater than or equal to 90% for M. beryllina and A. bahia.

The number of M. galloprovincialis control embryos that resulted in live larvae with completely developed shells at the end of the test was greater than 70%.

Therefore, the SPP tests passed the criteria for control survival.

C. Bioaccumulation Testing

Macoma nasuta

After 28 days, M. nasuta exposed to the composite resulted in the following survival:

M. nasuta Survival

99% 97% 96% 96%

Neanthes virens

After 28 days, N. virens exposed to the composite resulted in the following survival:

N. virens Survival

100% 100% 99% 100%

V. REFERENCES

1. USEPA/USACE, 1991. Evaluation of Dredged Material Proposed for Ocean Disposal, USEPA/ACOE, Washington, DC. EPA-503/8-91/001.

2. USEPA/NYACE, 1992. Draft Guidance for Performing Tests on Dredged

Material Proposed for Ocean Disposal. EPA Region II/ACOE New York District (Water Quality Compliance Section), New York, NY.

3. USEPA/NYACE, 2016. Final Guidance for Performing Tests on Dredged Material Proposed for Ocean Disposal. EPA Region II/ACOE New York District (Water Quality Compliance Section), New York, NY.

4. ASTM D422-63, Standard Test Method for Particle-Size Analysis of Soils, ASTM

1963, (reapproved 2002).

5. EPA Office of Solid Wastes & Emergency Response SW-846 Method No. 9060, Volume IC, Chapter 5, Revision 0, 9/86.

6. Blake G.R. and K. H. Hartge. 1986. Bulk Density. In Klute, A. (ed) 1986.

Methods of Soil Analysis Part I. Physical and Mineralogical Methods Bulk Density. 2nd ed. American Society of Agronomy, pp 363-375.

7. APHA, 1995. Standard Methods for the Examination of Water and Wastewater, APHA/AWWA/WPCF. Method 2710 F. Specific Gravity. pp 2-80.

8. ASTM D4318-00, Standard Test Methods for Liquid Limit, Plastic Limit, and

Plasticity Index of Soils, 2000.

9. ASTM, 1999. Standard Guide for Conducting 10-Day Sediment Toxicity Tests with Marine and Estuarine Amphipods, E1367-99. American Society of Testing and Materials, Philadelphia, PA.

10. T. Davies, D. Davis, and J. Elmore to EPA Regional Ocean Dumping

Coordinators, EPA Regional Wetland Coordinators, and Corps of Engineers Regulatory and Civil Works Elements, 1993. “Technical Panel Recommendations Concerning Use of Acute Amphipod Tests in Evaluation of Dredged Material”.

Draft Letter. [Photocopy]. New York District, Army Corps of Engineers, New York, NY.

11. Southerland, Elizabeth to Mario P. Del Vicario, June 14, 1994.

“Recommendations for Conducting Sediment Toxicity Test with Mysidopsis bahia when Ammonia may be Present at Toxic Levels”. [Photocopy]. New York, NY.

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Aqua Survey Inc.

469 Point Breeze Rd.

Flemington, NJ 08822

Tel: (908) 788-8700 Fax: (908) 788-9165 www.AquaSurvey.com

© OpenStreetMap (and) contributors, CC-BY-SA µ

ASI Project: 38-062

Coordinate System:

State Plane NAD 83 New Jersey US Survey Foot

ACOE-NYD

Raritan River

Sample Locations - Map

0 1,400 2,800 4,200700

Feet

Figure 1. Site Map

Table 1. Sample Identification and Compositing

Core Location ASI ID # Composite

RR-1 20180328

Reach A Composite 20180344

RR-2 20180329

RR-3 20180330

RR-4 20180331

RR-5 20180332

RR-6 20180333

RR-7 20180334

RR-8 20180335

RR-9 20180336

RR-10 20180337

Reach B Composite 20180345

RR-11 20180338

RR-12 20180339

RR-13 20180340

RR-14 20180341

Reach A Site Water 20180342

Reach B Site Water 20180343

Sandy Hook Control 20180359

Tuckerton Control 20180368

Nv Control 20180901

Mn Control 20180902

Mud Dump Reference 20180370

Table 2. DGPS Coordinates, Core Locations

Core Name Northings Eastings

RR-1 601652.5 562604.3

RR-2 601933.2 562342.6

RR-3 601755.3 562188.2

RR-4 601472.0 560652.5

RR-5 601442.3 560191.5

RR-6 601445.7 559664.4

RR-7 601464.5 558964.8

RR-8 602005.8 558405.2

RR-9 602405.2 557777.8

RR-10 605437.0 553444.3

RR-11 605912.0 552777.9

RR-12 608608.0 551539.4

RR-13 610486.6 549799.2

RR-14 610803.2 549268.3

Reach A Site Water 601532.4 559834.3

Reach B Site Water 607984.7 551745.7

Table 3. Test Performance Summary (% Survival)

Test Organism Control Reference Reach A Reach B

Solid Phase A. abdita 100% 99% 100% 96%

A. bahia 100% 98% 98% 96%

Conc. % Reach A Reach B

SPP M. beryllina 0 94%

10 97% 98%

50 70% 95%

100 0% 27%

A. bahia 0 90%

10 97% 92%

50 85% 90%

100 1% 84%

M. galloprovincialis 0 97.1%

10 88.8% 96.3%

50 72.0% 69.4%

100 11.9% 63.9%

Control Reference Reach A Reach B

Bioaccumulation M. nasuta 99% 97% 96% 96%

N. virens 100% 100% 99% 100%

Table 4a. Particle Size Distribution, Percent Moisture, and TOC Values of Test Samples

Sample ID ASI #

Gravel

Sand Silt

Clay

Moisture

TOC

ppm

% TOC

of Dry Weight

RR-1 20180328 0.0 27.8 46.6 25.6 60.0 37,714 3.77

RR-2 20180329 0.0 32.6 41.3 26.1 60.4 38,534 3.85

RR-3 20180330 0.0 33.3 40.5 26.2 59.9 36,038 3.60

RR-4 20180331 0.0 34.7 38.8 26.5 59.8 38,353 3.84

RR-5 20180332 0.0 33.6 44.3 22.1 62.1 41,187 4.12

RR-5 20180332 trp 0.0 35.1 42.3 22.6

RR-5 20180332 dup 0.0 35.3 41.2 23.5

RR-6 20180333 0.0 35.9 39.5 24.6 62.2 40,913 4.09

RR-7 20180334 0.0 32.1 42.4 25.5 59.1 39,898 3.99

RR-8 20180335 0.0 34.3 42.7 23.0 60.0 41,682 4.17

RR-9 20180336 0.0 29.6 47.5 22.9 61.5 40,625 4.06

RR-10 20180337 0.0 39.0 39.0 22.0 61.5 39,008 3.90

RR-11 20180338 0.0 32.7 45.6 21.7 59.9 37,433 3.74

RR-12 20180339 0.0 41.9 37.8 20.3 59.1 38,137 3.81

RR-13 20180340 2.0 32.5 49.6 15.9 62.8 61,642 6.16

RR-14 20180341 0.5 41.3 39.4 18.8 57.1 41,302 4.13

Reach A Composite 20180344 0.0 39.5 36.3 24.2 61.2 40,570 4.06

Reach B Composite 20180345 0.0 37.9 39.8 22.3 60.1 46,706 4.67

Reach B Composite 20180345 dup 59.2

Reach B Composite 20180345 trp 58.2

Table 4b. Particle Size Distribution, Percent Moisture, and TOC Values of Reference and Control Sediments

Table 5. Specific Gravity, Bulk Density, and Atterberg Limits

Sample ID ASI #

Gravel

Sand Silt

Clay

Moisture

TOC

ppm

% TOC

of Dry Weight

Sandy Hook Control 20180359 0.0 93.3 6.2 0.5 19.5 347 0.035

Tuckerton Control 20180368 0.0 18.9 46.6 34.5 62.5 34,061 3.41

Mud Dump Reference 20180370 0.0 93.3 6.2 0.5 17.9 622 0.062

Mud Dump Reference 20180370 dup 18.3

Mud Dump Reference 20180370 trp 17.5

Nv Control 20180901 0.0 63.6 24.5 11.9 42.1 18,340 1.83

Mn Control 20180902 16.6 76.0 4.9 2.5 15.9 2,394 0.240

Sample ID ASI # Specific Gravity

Bulk Density (g/mL)

Atterberg Limits (moisture content) wet dry Liquid Limit Plastic Limit

Reach A Comp 20180344 2.47 1.30 0.50 113 52

Reach A Comp 20180344 dup 2.43 113 52

Reach A Comp 2018344 trp 2.42 112 52

Mean 2.44 1.30 0.50 113 52

Reach B Comp 20180345 2.65 1.30 0.52 97 42

Reach B Comp 20180345 dup 2.61 1.31 0.52 97 42

Reach B Comp 2018345 trp 2.52 1.30 0.52 95 42

Mean 2.59 1.30 0.52 96 42

Table 6. Sampling Summary and Holding Times

Sample ASI # Sampling

Date Dates Used

Holding Time

Sandy Hook Control 20180359 5/29/18 7/9/18, A. bahia solid phase 41 days

Tuckerton Control 20180368 6/6/18 7/7/18, A. abdita solid phase 31 days

Nv Control 20180901* 7/2/18 7/6/18, N. virens bioaccumulation 4 days

Mn Control 20180902* 7/3/18 7/6/18, M. nasuta bioaccumulation 3 days

Mud Dump Reference 20180370 6/20/18

7/9/18, A. abdita solid phase 19 days

7/7/18, A. bahia solid phase 17 days

7/6/17, M. nasuta bioaccumulation 16 days

7/6/17, N. virens bioaccumulation 16 days

Reach A Composite 20180344 5/14/18

7/9/18, A. abdita solid phase 56 days

7/7/18, A. bahia solid phase 54 days

5/31/18, M. galloprovincialis suspended particulate phase 17 days

5/24/18, A. bahia suspended particulate phase 10 days

5/31/18, M. beryllina suspended particulate phase 17 days

7/6/18, M. nasuta bioaccumulation 53 days

7/6/18, N. virens bioaccumulation 53 days

Reach B Composite 20180345 5/16/18

7/9/18, A. abdita solid phase 54 days

7/7/18, A. bahia solid phase 52 days

5/31/18, M. galloprovincialis suspended particulate phase 15 days

5/24/18, A. bahia suspended particulate phase 8 days

5/31/18, M. beryllina suspended particulate phase 15 days

7/6/18, M. nasuta bioaccumulation 51 days

7/6/18, N. virens bioaccumulation 51 days

Reach A Site Water 20180342 5/21/18

5/31/18, M. galloprovincialis suspended particulate phase 10 days

5/24/18, A. bahia suspended particulate phase 3 days

5/31/18, M. beryllina suspended particulate phase 10 days

Reach B Site Water 20180343 5/21/18

5/31/18, M. galloprovincialis suspended particulate phase 10 days

5/24/18, A. bahia suspended particulate phase 3 days

5/31/18, M. beryllina suspended particulate phase 10 days

*ASI sample numbers assigned to subsamples for physical analyses

Table 7 10-Day Solid Phase Bioassay Species: A. abdita

ACOE: Raritan River Job #: 38-062 Initial Live Count: 20

Final Live Count

10 1.1 Tuckerton Control 20 9 1.2 20180368 20 1 1.3 20 16 1.4 20 11 1.5 20 100%

7 2.1 Mud Dump Reference 20 4 2.2 20180370 19

13 2.3 20 17 2.4 20 20 2.5 20 99%

15 3.1 Reach A Composite 20 12 3.2 20180344 20 2 3.3 20 5 3.4 20 19 3.5 20 100%

3 3.1 Reach B Composite 20 14 3.2 20180345 18 8 3.3 20 6 3.4 18 18 3.5 20 96%

Position # Code Sample Percent Survival

Table 8 10 Day Solid Phase Readings Species: A. abdita ACOE- Raritan River Job # : 38-062 Temperature ( ⁰ C )

Position # Code # Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

10 1.1 Tuckerton Control 19.3 19.6 19.6 19.8 19.7 19.8 19.8 19.3 19.0 19.5 19.9 9 1.2 20180368 19.5 19.8 19.8 20.0 19.8 19.9 19.9 19.4 19.0 19.6 19.8 1 1.3 19.7 19.9 20.1 20.2 20.2 20.2 20.0 19.6 19.3 19.8 20.1 16 1.4 19.4 19.8 19.7 19.9 19.9 19.9 20.0 19.4 19.1 19.6 20.0 11 1.5 19.3 19.5 19.5 19.7 19.6 19.7 19.9 19.4 19.2 19.6 19.8 19.0 20.2

7 2.1 Mud Dump Reference 19.3 19.6 19.4 19.8 19.5 19.7 19.8 19.2 18.9 19.4 19.7 4 2.2 20180370 19.4 19.7 19.7 19.9 19.6 19.7 19.7 19.2 18.8 19.4 19.8 13 2.3 19.3 19.7 19.6 19.8 19.6 19.7 19.8 19.3 18.9 19.4 19.8 17 2.4 19.5 20.0 19.9 20.0 19.7 19.9 19.9 19.4 19.1 19.6 19.7 20 2.5 19.5 19.8 19.7 19.9 19.6 19.8 20.0 19.3 18.9 19.8 20.0 18.8 20.0

15 3.1 Reach A Composite 19.3 19.6 19.6 19.8 19.6 19.8 20.1 19.5 19.0 19.5 19.9 12 3.2 20180344 19.4 19.7 19.6 19.9 19.6 19.9 20.1 19.5 19.1 19.5 19.8 2 3.3 19.5 19.8 19.8 20.0 19.6 19.9 19.9 19.4 19.0 19.6 19.9 5 3.4 19.5 19.8 19.8 20.0 19.7 19.9 19.9 19.4 19.0 19.6 19.8 19 3.5 19.4 19.7 19.7 19.9 19.6 19.8 20.2 19.5 19.1 19.6 20.0 19.0 20.2

3 3.1 Reach B Composite 19.5 19.7 19.7 19.9 19.6 19.9 20.0 19.4 18.9 19.5 19.9 14 3.2 20180345 19.3 19.5 19.4 19.7 19.4 19.6 20.0 19.5 19.2 19.7 19.8 8 3.3 19.5 19.7 19.6 19.9 19.6 19.8 20.0 19.4 19.1 19.6 19.8 6 3.4 19.2 19.6 19.5 19.8 19.5 19.6 20.0 19.3 18.9 19.4 19.8 18 3.5 19.4 19.7 19.5 19.8 19.4 19.7 * 19.6 19.0 19.6 19.9 18.9 20.0

Range 18.8 20.2 *- Chamber accidentally not read.

Table 9 10 Day Solid Phase Readings Species: A. abdita

Salinity (ppt)

Position # Code # Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

10 1.1 Tuckerton Control 29.4 29.6 29.5 29.5 29.5 29.6 29.4 29.7 29.3 29.6 29.8 9 1.2 20180368 29.4 29.6 29.6 29.5 29.6 29.7 29.7 29.8 29.8 29.9 29.9 1 1.3 29.3 29.6 29.5 29.5 29.4 29.7 29.6 29.6 29.6 29.9 29.7 16 1.4 29.5 29.5 29.4 29.4 29.1 29.6 29.1 29.6 29.7 29.3 29.7 11 1.5 29.5 29.6 29.4 29.5 29.6 29.7 28.9 29.8 29.8 29.6 29.9 28.9 29.9

7 2.1 Mud Dump Reference 29.5 29.6 29.5 29.6 29.5 29.5 29.0 29.7 29.7 29.7 29.8 4 2.2 20180370 29.5 29.7 29.7 29.6 29.7 29.3 29.8 30.0 30.0 30.0 30.1 13 2.3 29.5 29.6 29.6 29.6 29.7 29.7 29.5 29.7 29.6 29.8 29.9 17 2.4 29.5 29.6 29.6 29.7 29.6 29.8 29.5 29.9 29.9 29.8 30.0 20 2.5 29.5 29.6 29.6 29.6 29.5 29.7 29.2 29.9 29.9 29.9 30.0 29.0 30.1

15 3.1 Reach A Composite 29.5 29.7 28.8 29.6 29.7 29.7 29.6 29.9 29.9 29.9 30.0 12 3.2 20180344 29.5 29.6 29.6 29.6 29.4 29.6 29.4 29.8 29.8 29.9 29.6 2 3.3 29.6 29.8 29.8 29.8 29.9 29.7 30.0 30.1 30.0 30.0 30.0 5 3.4 29.6 29.7 29.6 29.8 29.8 29.7 29.0 30.0 29.6 30.0 29.7 19 3.5 29.5 29.7 29.7 29.7 29.5 29.8 29.8 29.9 29.4 29.9 30.1 28.8 30.1

3 3.1 Reach B Composite 29.4 29.6 29.7 29.6 29.6 29.8 28.9 29.7 29.9 29.9 29.9 14 3.2 20180345 29.5 29.6 29.6 29.6 29.5 29.6 29.1 29.7 29.8 29.7 29.6 8 3.3 29.4 29.6 29.6 29.5 29.4 29.6 28.8 29.7 29.7 29.8 29.9 6 3.4 29.5 29.5 29.5 29.5 29.5 29.6 29.6 29.7 29.7 29.6 29.8 18 3.5 29.6 29.7 29.7 29.6 29.6 29.3 * 29.8 29.7 29.7 29.9 28.8 29.9

Range 28.8 30.1

Table 10 10 Day Solid Phase Readings Species: A. abdita

DO (mg/L)

10 1.1 Tuckerton Control 7.34 7.53 7.47 7.23 6.84 6.81 7.04 7.08 7.06 6.86 6.99 9 1.2 20180368 7.27 7.48 7.41 7.20 6.79 6.92 7.03 7.05 7.05 6.91 6.95 1 1.3 7.24 7.52 7.50 7.23 6.78 6.81 7.02 7.01 7.15 6.83 6.91 16 1.4 7.31 7.51 7.60 7.24 6.70 6.82 6.98 7.07 7.07 6.90 6.90 11 1.5 7.31 7.57 7.55 7.23 6.83 6.83 6.98 6.99 6.91 6.81 6.98 6.70 7.60

7 2.1 Mud Dump Reference 7.34 7.52 7.69 7.25 6.97 6.88 7.05 7.17 7.08 6.88 7.03 4 2.2 20180370 7.21 7.54 7.64 7.30 6.98 6.95 7.07 7.20 7.18 6.89 7.01 13 2.3 7.35 7.53 7.45 7.20 6.68 6.85 7.00 7.15 7.11 6.88 7.08 17 2.4 7.30 7.50 7.48 7.17 6.77 6.83 7.01 7.13 7.05 6.83 7.10 20 2.5 7.39 7.58 7.59 7.25 6.97 6.92 7.01 7.17 7.12 6.95 7.10 6.68 7.69

15 3.1 Reach A Composite 6.99 7.46 7.52 6.89 6.80 6.73 6.86 6.48 6.87 6.72 6.79 12 3.2 20180344 6.73 7.23 7.35 6.81 6.82 6.53 6.63 6.19 6.77 6.71 6.70 2 3.3 6.70 7.24 7.46 6.44 6.59 6.66 6.95 6.95 6.71 6.81 6.91 5 3.4 6.54 7.08 7.28 6.33 6.49 6.65 6.75 6.86 6.62 6.55 6.48 19 3.5 6.90 7.39 7.45 6.99 6.85 6.77 6.77 6.57 6.81 6.73 6.86 6.19 7.52

3 3.1 Reach B Composite 7.22 7.43 7.65 7.19 6.92 6.68 7.08 7.03 6.96 6.80 6.73 14 3.2 20180345 7.27 7.54 7.66 7.14 6.88 6.90 6.88 6.92 6.86 6.72 6.90 8 3.3 7.18 7.55 7.50 7.09 6.88 6.80 6.89 6.44 6.83 6.65 6.63 6 3.4 7.27 7.45 7.56 7.09 6.75 6.90 6.78 6.99 6.75 6.74 6.77 18 3.5 7.23 7.38 7.57 7.13 6.97 6.84 * 6.92 6.99 6.79 6.86 6.44 7.66

Range 6.19 7.69

Table 11 10 Day Solid Phase Readings Species: A. abdita pH (SU)

Position # Code # Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

10 1.1 Tuckerton Control 7.76 7.81 7.60 7.73 7.88 7.95 7.96 8.04 7.97 8.01 7.91 9 1.2 20180368 7.75 7.79 7.54 7.70 7.87 7.93 7.93 8.02 7.96 7.98 7.86 1 1.3 7.77 7.79 7.41 7.65 7.86 7.93 7.91 7.99 7.87 7.95 7.76 16 1.4 7.76 7.83 7.68 7.78 7.88 7.96 7.99 8.07 8.00 8.04 8.03 11 1.5 7.76 7.83 7.64 7.76 7.89 7.95 7.97 8.06 7.98 8.03 7.95 7.41 8.07

7 2.1 Mud Dump Reference 7.81 7.83 7.73 7.76 7.85 7.92 7.90 7.98 7.92 7.97 7.92 4 2.2 20180370 7.80 7.83 7.72 7.78 7.87 7.94 7.94 8.02 7.98 8.03 7.97 13 2.3 7.81 7.82 7.73 7.76 7.82 7.89 7.88 7.96 7.89 7.95 7.90 17 2.4 7.82 7.82 7.73 7.75 7.81 7.87 7.87 7.95 7.87 7.94 7.89 20 2.5 7.83 7.84 7.75 7.76 7.81 7.87 7.86 7.96 7.88 7.95 7.90 7.72 8.03

15 3.1 Reach A Composite 7.66 7.71 7.65 7.61 7.65 7.73 7.73 7.87 7.78 7.92 7.93 12 3.2 20180344 7.67 7.71 7.66 7.62 7.65 7.74 7.75 7.86 7.76 7.90 7.92 2 3.3 7.75 7.80 7.72 7.70 7.76 7.82 7.83 7.93 7.70 7.95 7.90 5 3.4 7.70 7.74 7.69 7.64 7.70 7.79 7.78 7.92 7.77 7.92 7.88 19 3.5 7.66 7.70 7.64 7.61 7.66 7.73 7.73 7.88 7.80 7.94 7.93 7.61 7.95

3 3.1 Reach B Composite 7.67 7.70 7.63 7.61 7.65 7.72 7.74 7.90 7.81 7.94 7.93 14 3.2 20180345 7.68 7.71 7.64 7.63 7.69 7.75 7.77 7.91 7.83 7.91 7.90 8 3.3 7.68 7.70 7.63 7.62 7.67 7.75 7.76 7.89 7.80 7.90 7.89 6 3.4 7.71 7.69 7.64 7.62 7.67 7.74 7.75 7.90 7.80 7.92 7.90 18 3.5 7.68 7.70 7.64 7.63 7.69 7.75 * 7.91 7.83 7.92 7.90 7.61 7.94

Range 7.41 8.07

Table 12 10 Day Solid Phase Readings Species: A. abdita

Total Ammonia (mg/L)

Position # Code # Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

10 1.1 Tuckerton Control 0.41 0.82 9 1.2 20180368 0.37 1.03 1 1.3 0.40 1.16 16 1.4 0.44 0.38 11 1.5 0.41 0.76 0.37 1.16

7 2.1 Mud Dump Reference <0.20 <0.20 4 2.2 20180370 <0.20 <0.20 13 2.3 <0.20 <0.20 17 2.4 <0.20 <0.20 20 2.5 <0.20 <0.20 <0.20 <0.20

15 3.1 Reach A Composite 0.94 <0.20 12 3.2 20180344 1.79 <0.20 2 3.3 1.29 <0.20 5 3.4 1.53 <0.20 19 3.5 1.11 <0.20 <0.20 1.79

3 3.1 Reach B Composite 1.55 1.71 14 3.2 20180345 1.30 0.77 8 3.3 1.45 1.07 6 3.4 1.53 1.76 18 3.5 1.21 0.69 0.69 1.76

Range <0.20 1.79

Table 13 10-Day Solid Phase Bioassay Species: A. bahia

ACOE: Raritan River Job #: 38-062 Initial Live Count: 20

Final Live Count

10 1.1 Sandy Hook Control 20 9 1.2 20180359 20 1 1.3 20 16 1.4 20 11 1.5 20 100%

7 2.1 Mud Dump Reference 19 4 2.2 20180370 20

13 2.3 20 17 2.4 20 20 2.5 19 98%

15 3.1 Reach A Composite 19 12 3.2 20180344 19 2 3.3 20 5 3.4 20 19 3.5 20 98%

3 3.1 Reach B Composite 19 14 3.2 20180345 20 8 3.3 19 6 3.4 19 18 3.5 19 96%

Position # Code Sample Percent

Table 14 10 Day Solid Phase Readings Species: A. bahia

Temperature (⁰ C )

10 1.1 Sandy Hook Control 19.9 19.8 19.5 19.4 19.4 19.3 19.1 19.5 19.4 19.3 19.9 9 1.2 20180359 19.9 19.9 19.5 19.4 19.4 19.4 19.1 19.6 19.3 19.3 19.9 1 1.3 19.8 20.0 19.4 19.5 19.3 19.6 19.0 19.6 19.3 19.3 19.9 16 1.4 19.9 19.8 19.5 19.3 19.4 19.3 19.1 19.4 19.3 19.3 19.9 11 1.5 19.9 19.8 19.5 19.3 19.4 19.3 19.1 19.4 19.4 19.3 19.9 19.0 20.0

7 2.1 Mud Dump Reference 19.9 19.8 19.5 19.3 19.4 19.3 19.1 19.4 19.3 19.3 19.9 4 2.2 20180370 19.8 19.7 19.4 19.2 19.4 19.3 19.1 19.4 19.3 19.3 19.9 13 2.3 19.9 19.8 19.5 19.3 19.4 19.3 19.1 19.4 19.4 19.3 19.9 17 2.4 19.9 19.8 19.5 19.3 19.5 19.3 19.1 19.4 19.3 19.3 19.9 20 2.5 19.9 19.8 19.5 19.3 19.5 19.3 19.1 19.4 19.4 19.3 19.9 19.1 19.9

15 3.1 Reach A Composite 19.9 19.8 19.5 19.3 19.4 19.3 19.0 19.3 19.3 19.3 20.0 12 3.2 20180344 19.9 19.8 19.5 19.2 19.4 19.3 19.0 19.4 19.3 19.3 20.0 2 3.3 19.8 19.8 19.4 19.3 19.4 19.2 19.0 19.3 19.3 19.3 19.9 5 3.4 19.9 19.8 19.5 19.3 19.5 19.2 19.1 19.3 19.3 19.3 20.0 19 3.5 19.9 19.8 19.5 19.2 19.3 19.3 19.0 19.4 19.4 19.3 20.0 19.0 20.0

3 3.1 Reach B Composite 19.8 19.7 19.4 19.2 19.4 19.2 19.0 19.3 19.3 19.3 20.0 14 3.2 20180345 19.9 19.8 19.5 19.3 19.4 19.2 19.1 19.4 19.4 19.3 20.0 8 3.3 19.9 19.8 19.5 19.3 19.4 19.2 19.1 19.3 19.4 19.3 20.1 6 3.4 19.9 19.8 19.5 19.3 19.5 19.2 19.1 19.4 19.4 19.3 20.0 18 3.5 19.9 19.8 19.5 19.2 19.4 19.3 19.1 19.4 19.4 19.3 20.1 19.0 20.1

Range 19.0 20.1

Table 15 10 Day Solid Phase Readings Species: A. bahia

Salinity (ppt)

Position # Code # Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

10 1.1 Sandy Hook Control 29.7 29.7 29.6 29.7 29.6 29.8 29.5 29.8 29.8 29.8 29.9 9 1.2 20180359 29.7 29.7 29.7 29.9 29.7 29.9 29.6 29.9 29.5 29.9 30.0 1 1.3 29.7 29.7 29.8 29.9 29.8 29.9 29.1 29.9 29.8 29.9 29.9 16 1.4 29.7 29.7 29.4 29.7 29.7 29.9 29.7 29.9 29.9 29.9 30.1 11 1.5 29.7 29.7 29.5 29.7 29.5 29.8 29.5 29.8 29.8 29.8 29.9 29.1 30.1

7 2.1 Mud Dump Reference 29.6 29.6 29.5 29.6 29.5 29.7 29.5 29.7 29.7 29.8 29.8 4 2.2 20180370 29.7 29.6 29.5 29.7 29.5 29.8 29.4 29.8 29.9 29.9 29.9 13 2.3 29.6 29.6 29.5 29.6 29.5 29.7 29.6 29.7 29.7 29.7 29.8 17 2.4 29.6 29.6 29.5 29.6 29.5 29.6 29.5 29.6 29.6 29.6 29.7 20 2.5 29.6 29.6 29.5 29.7 29.5 29.7 29.6 29.6 29.7 29.7 29.8 29.4 29.9

15 3.1 Reach A Composite 29.7 29.8 29.5 29.8 29.7 29.8 29.8 29.7 30.0 30.0 30.2 12 3.2 20180344 29.7 29.7 29.5 29.6 29.3 29.8 29.7 29.4 29.8 29.8 30.0 2 3.3 29.7 29.7 29.5 29.6 29.6 29.7 29.5 29.7 29.9 29.8 30.0 5 3.4 29.6 29.7 29.5 29.6 29.3 29.7 29.7 29.7 29.8 29.8 30.0 19 3.5 29.7 29.6 29.5 29.6 29.6 29.8 29.6 29.6 29.6 29.8 29.9 29.3 30.2

3 3.1 Reach B Composite 29.8 29.7 29.5 29.8 29.7 29.8 28.8 29.7 29.9 29.9 30.0 14 3.2 20180345 29.7 29.6 29.4 29.4 29.4 29.7 29.6 29.5 29.7 29.8 29.8 8 3.3 29.7 29.6 29.6 29.6 29.6 29.7 29.6 28.8 29.8 29.8 29.9 6 3.4 29.6 29.6 29.5 29.6 29.5 29.7 29.6 29.7 29.7 29.8 29.9 18 3.5 29.6 29.7 29.5 29.6 29.6 29.8 29.8 29.5 29.6 29.9 30.0 28.8 30.0

Range 28.8 30.2

Table 16 10 Day Solid Phase Readings Species: A. bahia

DO (mg/L)

10 1.1 Sandy Hook Control 7.65 6.88 6.76 6.76 6.80 6.86 6.60 6.62 6.82 6.84 4.67 9 1.2 20180359 7.67 7.23 6.80 6.86 6.96 7.00 6.87 6.78 6.91 6.90 5.34 1 1.3 7.67 7.26 6.89 6.96 6.79 6.95 6.83 6.78 6.89 6.85 5.17 16 1.4 7.66 7.19 6.79 6.89 6.83 6.96 6.82 6.89 6.97 7.06 4.87 11 1.5 7.67 7.17 6.77 6.78 6.76 6.86 6.66 6.78 6.86 6.97 4.94 4.67 7.67

7 2.1 Mud Dump Reference 7.64 7.11 6.82 6.85 6.82 6.81 6.65 6.83 6.82 6.97 4.62 4 2.2 20180370 7.66 7.34 6.98 7.02 7.02 6.92 6.85 6.87 7.03 6.99 4.80 13 2.3 7.63 7.11 6.75 6.75 6.69 6.79 6.76 6.78 6.75 6.79 4.58 17 2.4 7.65 7.08 6.69 6.58 6.30 6.54 6.69 6.69 6.80 6.84 4.18 20 2.5 7.65 7.13 6.64 6.67 6.52 6.83 6.76 6.71 6.82 6.87 4.36 4.18 7.66

15 3.1 Reach A Composite 7.32 6.88 6.67 6.52 6.72 6.70 6.61 6.63 6.80 6.88 4.46 12 3.2 20180344 7.33 6.97 6.67 6.72 6.96 6.73 6.43 6.47 6.61 6.43 4.25 2 3.3 7.48 7.07 6.79 6.77 6.84 6.41 6.82 6.76 6.81 6.71 4.19 5 3.4 7.39 6.98 6.63 6.63 6.76 6.67 6.62 6.87 6.79 6.71 4.22 19 3.5 7.38 6.82 6.50 6.46 6.59 6.67 6.66 6.68 6.29 5.69 4.46 4.19 7.48

3 3.1 Reach B Composite 7.51 7.06 6.80 6.78 6.93 6.23 6.76 6.91 6.86 6.63 4.08 14 3.2 20180345 7.31 7.05 6.57 6.55 6.57 6.55 6.61 6.65 6.40 5.54 4.58 8 3.3 7.39 6.89 6.60 6.43 6.54 6.48 6.49 6.74 6.70 5.71 4.12 6 3.4 7.43 7.04 6.70 6.73 6.50 6.42 6.74 6.74 6.82 6.23 4.21 18 3.5 7.28 7.00 6.69 6.64 6.66 6.72 6.68 6.70 6.70 5.81 4.44 4.08 7.51

Range 4.08 7.67

Table 17 10 Day Solid Phase Readings Species: A. bahia pH (SU)

Position # Code # Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

10 1.1 Sandy Hook Control 7.82 7.69 7.78 7.86 7.72 7.84 7.58 7.80 7.79 7.76 7.70 9 1.2 20180359 7.81 7.69 7.79 7.88 7.74 7.86 7.58 7.87 7.81 7.76 7.73 1 1.3 7.80 7.69 7.80 7.93 7.75 7.87 7.47 7.87 7.85 7.78 7.77 16 1.4 7.83 7.69 7.76 7.83 7.70 7.82 7.64 7.81 7.79 7.77 7.70 11 1.5 7.83 7.69 7.77 7.84 7.71 7.83 7.59 7.80 7.78 7.75 7.70 7.47 7.93

7 2.1 Mud Dump Reference 7.83 7.70 7.76 7.80 7.71 7.81 7.66 7.80 7.77 7.77 7.69 4 2.2 20180370 7.83 7.70 7.77 7.82 7.71 7.82 7.66 7.80 7.79 7.76 7.69 13 2.3 7.84 7.70 7.75 7.79 7.69 7.80 7.64 7.78 7.74 7.73 7.66 17 2.4 7.83 7.69 7.74 7.77 7.65 7.75 7.63 7.76 7.72 7.71 7.62 20 2.5 7.84 7.68 7.73 7.75 7.64 7.74 7.62 7.76 7.72 7.73 7.62 7.62 7.84

15 3.1 Reach A Composite 7.73 7.61 7.67 7.71 7.64 7.79 7.69 7.77 7.80 7.87 7.79 12 3.2 20180344 7.74 7.62 7.69 7.73 7.66 7.81 7.68 7.78 7.79 7.80 7.77 2 3.3 7.80 7.66 7.72 7.74 7.65 7.83 7.70 7.78 7.78 7.82 7.75 5 3.4 7.77 7.64 7.70 7.73 7.66 7.81 7.69 7.79 7.81 7.85 7.77 19 3.5 7.72 7.60 7.65 7.69 7.62 7.78 7.68 7.77 7.76 7.83 7.76 7.60 7.87

3 3.1 Reach B Composite 7.70 7.58 7.63 7.66 7.62 7.73 7.65 7.74 7.72 7.77 7.68 14 3.2 20180345 7.68 7.54 7.58 7.59 7.55 7.65 7.58 7.69 7.67 7.68 7.55 8 3.3 7.68 7.55 7.59 7.62 7.56 7.70 7.60 7.71 7.70 7.73 7.57 6 3.4 7.69 7.56 7.61 7.65 7.59 7.71 7.62 7.74 7.72 7.76 7.64 18 3.5 7.67 7.54 7.58 7.59 7.55 7.66 7.57 7.67 7.67 7.69 7.55 7.54 7.77

Range 7.47 7.93

Table 18 10 Day Solid Phase Readings Species: A. bahia

Total Ammonia (mg/L)

Position # Code # Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

10 1.1 Sandy Hook Control <0.20 0.93 4.45 5.48 9 1.2 20180359 <0.20 0.28 3.14 3.97 1 1.3 <0.20 <0.20 1.70 3.26 16 1.4 <0.20 1.48 4.88 11 1.5 <0.20 1.07 4.39 4.07 <0.20 5.48

7 2.1 Mud Dump Reference <0.20 0.48 3.09 5.18 4 2.2 20180370 <0.20 <0.20 2.38 5.45 13 2.3 <0.20 0.73 3.33 4.83 17 2.4 <0.20 1.10 6.30 6.52 20 2.5 <0.20 2.00 6.45 <0.20 6.52

15 3.1 Reach A Composite 0.34 0.51 0.69 0.86 0.93 0.94 0.71 0.49 0.39 0.28 <0.20 12 3.2 20180344 0.26 0.37 0.55 0.59 0.47 0.36 0.53 0.62 0.65 0.47 0.22 2 3.3 0.44 0.38 0.47 0.49 0.51 0.59 0.76 0.69 0.47 0.34 <0.20 5 3.4 0.30 0.31 0.48 0.45 0.53 0.51 0.66 0.53 0.42 0.30 <0.20 19 3.5 0.25 0.39 0.33 0.50 0.37 0.28 0.48 1.21 1.56 1.20 0.76 <0.20 1.56

3 4.1 Reach B Composite 1.16 1.79 2.20 2.34 3.63 4.55 5.92 6.85 7.41 7.23 7.30 14 4.2 20180345 1.18 1.87 2.14 2.43 3.92 4.01 5.92 7.20 8.21 7.29 8.74 8 4.3 1.20 1.94 1.89 2.17 3.66 3.56 5.25 6.46 6.70 6.09 6.57 6 4.4 1.24 1.85 1.99 2.44 3.61 4.19 5.42 6.28 6.29 6.05 4.91 18 4.5 1.05 1.70 1.67 1.78 3.00 3.17 4.92 6.17 5.85 5.03 5.11 1.05 8.74

Range <0.20 8.74

Table 19 10 Day Solid Phase Readings Species: A. bahia

Unionized Ammonia (mg/L- Calculated)

Position # Code # Sample 0 1 2 3 4 5 6 7 8 9 10 Low High

10 1.1 Sandy Hook Control 0.00 0.02 0.08 0.09 9 1.2 20180359 0.01 0.07 1 1.3 0.00 0.01 16 1.4 0.03 11 1.5 0.02 0.07 0.00 0.09

7 2.1 Mud Dump Reference 0.00 0.01 0.06 4 2.2 20180370 0.00 0.03 13 2.3 0.01 0.06 17 2.4 0.02 0.10 0.09 20 2.5 0.03 0.00 0.09

15 3.1 Reach A Composite 0.01 0.01 0.01 0.01 0.01 0.02 0.01 0.01 0.01 0.01 0.00 12 3.2 20180344 0.00 0.00 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.00 2 3.3 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.00 5 3.4 0.01 0.00 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.00 19 3.5 0.00 0.00 0.00 0.01 0.00 0.01 0.01 0.02 0.03 0.02 0.01 0.00 0.03

3 4.1 Reach B Composite 0.02 0.02 0.03 0.03 0.05 0.07 0.08 0.11 0.12 0.13 0.11 14 4.2 20180345 0.02 0.02 0.02 0.03 0.04 0.05 0.07 0.11 0.12 0.11 0.10 8 4.3 0.02 0.02 0.02 0.03 0.04 0.05 0.06 0.10 0.10 0.10 0.08 6 4.4 0.02 0.02 0.02 0.03 0.04 0.06 0.07 0.10 0.10 0.10 0.07 18 4.5 0.02 0.02 0.02 0.02 0.03 0.04 0.05 0.09 0.08 0.07 0.06 0.02 0.13

Range 0.00 0.13

Table 20 96-hour Suspended Particulate Phase Species: M. beryllina

ACOE- Raritan River Job #: 38-062 Initial Live Count: 20

Position # Code Sample Final Live Count Percent Survival LC50

1 A Control 20 27 B 19 14 C 20 13 D 19 16 E 16 94%

3 A Reach A Composite 20

29 B 10% SPP 18

22 C 19 30 D 20 9 E 20 97%

21 A Reach A Composite 14

2 B 50% SPP 14

24 C 14 35 D 15 31 E 13 70%

23 A Reach A Composite 0

4 B 100% SPP 0

8 C 0

25 D 0 20 E 0 0% 52.0%

6 A Reach B Composite 20

26 B 10% SPP 20

18 C 19 10 D 19 19 E 20 98%

34 A Reach B Composite 19

17 B 50% SPP 19

28 C 18 5 D 19

32 E 20 95%

11 A Reach B Composite 8

12 B 100% SPP 4

33 C 5 7 D 5

15 E 5 27% 81.0%

Table 21 96 Hour Suspended Particulate Phase Test Species: M. beryllina ACOE- Raritan River Job No. 38-062 Temperature (° C)

Position # Code Sample 0 24 48 72 96 Low High

1 A Control 22.0 20.4 20.3 20.2 19.8 27 B 20.3 20.3 20.3 19.7 14 C 20.3 20.3 20.3 19.7 13 D 20.4 20.3 20.3 19.7 16 E 20.3 20.3 20.3 19.7 19.7 22.0

3 A Reach A Composite 21.8 20.3 20.3 20.2 19.7

29 B 10% SPP 20.3 20.3 20.3 19.7

22 C 20.3 20.3 20.2 19.7 30 D 20.3 20.3 20.2 19.7 9 E 20.3 20.3 20.2 19.7 19.7 21.8

21 A Reach A Composite 21.0 20.3 20.3 20.2 19.7

2 B 50% SPP 20.3 20.3 20.2 19.7

24 C 20.3 20.3 20.2 19.7 35 D 20.3 20.3 20.2 19.7 31 E 20.3 20.3 20.2 19.7 19.7 21.0

23 A Reach A Composite 19.9 20.3 20.3

4 B 100% SPP 20.3 20.3

8 C 20.3 20.3

25 D 20.2 20.3 20 E 20.3 20.3 19.9 20.3

6 A Reach B Composite 21.5 20.3 20.3 20.2 19.7

26 B 10% SPP 20.3 20.3 20.2 19.7

18 C 20.3 20.3 20.2 19.7 10 D 20.3 20.3 20.2 19.7 19 E 20.4 20.3 20.2 19.7 19.7 21.5

34 A Reach B Composite 20.7 20.3 20.3 20.2 19.7

17 B 50% SPP 20.4 20.3 20.2 19.7

28 C 20.4 20.3 20.2 19.7 5 D 20.3 20.3 20.2 19.7

32 E 20.4 20.3 20.2 19.7 19.7 20.7

11 A Reach B Composite 19.6 20.4 20.3 20.2 19.7

12 B 100% SPP 20.4 20.3 20.2 19.7

33 C 20.3 20.3 20.2 19.7 7 D 20.4 20.3 20.2 19.7

15 E 20.4 20.3 20.2 19.7 19.6 20.4 Range 19.6 22.0

Table 22 96 Hour Suspended Particulate Phase Test Species: M. beryllina

Salinity (ppt)

Position # Code Sample 0 24 48 72 96 Low High

1 A Control 29.3 29.5 29.5 29.5 29.6 27 B 29.5 29.5 29.5 29.6 14 C 29.5 29.5 29.5 29.6 13 D 29.5 29.5 29.5 29.6 16 E 29.5 29.4 29.5 29.6 29.3 29.6

3 A Reach A Composite 29.4 29.5 29.5 29.5 29.6

29 B 10% SPP 29.5 29.5 29.5 29.6

22 C 29.5 29.5 29.4 29.6 30 D 29.5 29.5 29.5 29.6 9 E 29.5 29.5 29.5 29.6 29.4 29.6

21 A Reach A Composite 29.4 29.4 29.5 29.5 29.5

2 B 50% SPP 29.5 29.5 29.5 29.6

24 C 29.5 29.5 29.5 29.6 35 D 29.5 29.5 29.5 29.6 31 E 29.5 29.5 29.5 29.6 29.4 29.6

23 A Reach A Composite 29.4 29.5 29.5

4 B 100% SPP 29.4 29.5

8 C 29.5 29.5

25 D 29.4 29.5 20 E 29.5 29.5 29.4 29.5

6 A Reach B Composite 29.3 29.4 29.4 29.4 29.4

26 B 10% SPP 29.4 29.4 29.4 29.5

18 C 29.4 29.4 29.4 29.5 10 D 29.4 29.4 29.4 29.5 19 E 29.4 29.4 29.4 29.5 29.3 29.5

34 A Reach B Composite 29.4 29.4 29.5 29.4 29.5

17 B 50% SPP 29.4 29.5 29.4 29.5

28 C 29.4 29.5 29.4 29.5 5 D 29.4 29.5 29.4 29.5

32 E 29.4 29.5 29.4 29.6 29.4 29.6

11 A Reach B Composite 29.3 29.4 29.4 29.4 29.5

12 B 100% SPP 29.4 29.4 29.4 29.5

33 C 29.4 29.4 29.4 29.5 7 D 29.4 29.4 29.4 29.5

15 E 29.4 29.4 29.4 29.5 29.3 29.5 Range 29.3 29.6

Table 23 96 Hour Suspended Particulate Phase Test Species: M. beryllina

D.O. (mg/L)

Position # Code Sample 0 24 48 72 96 Low High

1 A Control 6.71 6.08 5.80 5.42 5.53 27 B 6.08 5.75 5.19 5.84 14 C 6.02 5.66 4.89 5.60 13 D 5.97 5.51 4.54 5.59 16 E 6.02 5.71 4.98 5.76 4.54 6.71

3 A Reach A Composite 6.71 6.11 5.83 5.39 5.67

29 B 10% SPP 5.74 5.46 4.86 5.34

22 C 5.81 5.58 5.07 5.68 30 D 5.68 5.14 4.45 5.03 9 E 6.07 5.60 5.18 5.85 4.45 6.71

21 A Reach A Composite 6.66 5.57 5.34 4.54 5.24

2 B 50% SPP 5.64 5.23 4.89 5.43

24 C 5.65 5.35 4.32 4.65 35 D 5.91 5.23 4.21 4.66 31 E 5.65 5.46 4.35 4.97 4.21 6.66

23 A Reach A Composite 6.24 5.56 5.51

4 B 100% SPP 5.61 5.81

8 C 5.74 5.83

25 D 6.26 5.40 20 E 5.80 5.84 5.40 6.26

6 A Reach B Composite 6.64 6.15 5.96 5.26 5.60

26 B 10% SPP 5.92 5.80 4.88 5.43

18 C 5.85 5.93 4.90 5.65 10 D 5.95 5.99 5.11 5.89 19 E 5.92 5.73 4.79 5.46 4.79 6.64

34 A Reach B Composite 6.55 5.67 5.21 4.38 5.04

17 B 50% SPP 5.69 5.32 4.52 5.17

28 C 5.65 5.57 4.50 4.97 5 D 5.73 5.49 4.58 5.07

32 E 5.54 5.44 4.50 5.13 4.38 6.55

11 A Reach B Composite 5.92 5.64 5.35 5.28 5.83

12 B 100% SPP 5.56 5.26 5.24 5.91

33 C 5.51 5.42 5.13 5.73 7 D 5.83 5.40 5.21 5.64

15 E 5.73 5.13 4.99 5.80 4.99 5.92 Range 4.21 6.71

Table 24 96 Hour Suspended Particulate Phase Test Species: M. beryllina pH

Position # Code Sample 0 24 48 72 96 Low High

1 A Control 7.95 7.77 7.79 7.78 7.73 27 B 7.75 7.73 7.67 7.70 14 C 7.76 7.74 7.68 7.70 13 D 7.77 7.75 7.69 7.71 16 E 7.76 7.74 7.68 7.71 7.67 7.95

3 A Reach A Composite 7.95 7.81 7.75 7.72 7.82

29 B 10% SPP 7.84 7.80 7.76 7.82

22 C 7.83 7.79 7.76 7.82 30 D 7.83 7.79 7.75 7.81 9 E 7.82 7.77 7.74 7.82 7.72 7.95

21 A Reach A Composite 7.96 7.92 7.92 7.96 8.08

2 B 50% SPP 7.89 7.86 7.95 8.08

24 C 7.94 7.94 7.97 8.08 35 D 8.01 7.96 7.98 8.08 31 E 7.94 7.95 7.98 8.09 7.86 8.09

23 A Reach A Composite 7.99 8.04 8.07

4 B 100% SPP 8.02 8.04

8 C 8.03 8.05

25 D 7.99 8.07 20 E 8.04 8.06 7.99 8.07

6 A Reach B Composite 7.99 7.95 7.95 7.87 7.87

26 B 10% SPP 7.99 7.88 7.78 7.84

18 C 7.90 7.91 7.80 7.85 10 D 7.91 7.93 7.84 7.87 19 E 7.89 7.89 7.79 7.84 7.78 7.99

34 A Reach B Composite 8.04 8.07 7.99 7.94 8.03

17 B 50% SPP 8.01 7.96 7.92 8.03

28 C 8.01 7.97 7.93 8.03 5 D 8.00 7.92 7.90 8.03

32 E 8.01 7.99 7.95 8.03 7.90 8.07

11 A Reach B Composite 8.12 8.10 8.07 8.08 8.23

12 B 100% SPP 8.11 8.08 8.09 8.22

33 C 7.99 8.10 8.10 8.22 7 D 8.10 8.06 8.07 8.22

15 E 8.12 8.10 8.10 8.22 7.99 8.23 Range 7.67 8.23

Table 25 96 Hour Suspended Particulate Phase Test Species: M. beryllina

Total Ammonia (mg/L)

Position # Code Sample 0 24 48 72 96 Low High

1 A Control <0.20 0.31 27 B 0.33 14 C <0.20 0.37 13 D <0.20 0.47 16 E 0.27 0.28 <0.20 0.47

3 A Reach A Composite 1.33 2.68

29 B 10% SPP 2.76 2.88

22 C 1.40 2.76 30 D 3.00 9 E 1.31 2.75 1.31 3.00

21 A Reach A Composite 6.15 13.3

2 B 50% SPP 6.20 14.8

24 C 6.15 13.5 35 D 14.2 31 E 17.9 13.2 6.15 17.