Project Grant R43GM133247

This Project Grant awarded by the National Institute of Environmental Health Sciences (NIEHS) under the Environmental Health (CFDA 93.113) program provides $295,734 to Litron Laboratories Ltd, a for-profit biotechnology company, to develop a comprehensive hen's egg model for genotoxicity testing. The project aims to create a non-animal, metabolically-active in vitro system that can effectively assess the genotoxic risk of pharmaceuticals and industrial chemicals. Key deliverables include...

The National Institutes of Health (NIH) Office of Research Infrastructure Programs (ORIP) awarded a $775,888 Project Grant (CFDA 93.351) to the La Jolla Institute for Immunology to develop a next-generation CRISPR-ready, HLA class I and II transgenic mouse platform. The goal is to create a modular mouse model that can be easily customized with different HLA alleles to study a broad range of diseases involving T cell responses, from infection and cancer to autoimmune conditions and transplant...

This Project Grant award of $240,039 from the National Institutes of Health's Office of Research Infrastructure Programs (CFDA 93.351 - Research Infrastructure Programs) supports the development of a novel mouse model for detecting genome editing events using CRISPR/Cas9 technology. The project aims to design and test a "single-hit" approach that utilizes Cre recombinase fused to an estrogen receptor ligand binding domain (ERT2) to provide a more sensitive system for detecting a...

This Project Grant award, provided by the National Institutes of Health (NIH) under the Research Infrastructure Programs (CFDA 93.351), aims to rapidly develop advanced mouse resource strains through the application of Prime Editing technology. The $265,500 award, granted on September 1, 2024, will fund research at the Jackson Laboratory to create a novel combinatorial system that can efficiently modify thousands of mouse genes by applying Prime Editing techniques. The key innovation is the...

The National Human Genome Research Institute (CFDA 93.172 - Human Genome Research) awarded Panluminate Inc., a woman-owned small business, a $404,117 Project Grant (R43HG014432) to develop CHROMEX30, a novel variant of the company's proprietary Pan-Expansion Microscopy (PAN-EXM) technology. CHROMEX30 is designed to enable nanoscale imaging of genetic organization and disease phenotypes at a resolution greater than 30x expansion, offering significant advantages over traditional electron...

This federal Project Grant award from the National Human Genome Research Institute (CFDA 93.172 - Human Genome Research) provides $383,748 to Jumpcode Genomics Inc. to develop a CRISPR-based depletion technology to resolve pseudogene contamination in next-generation sequencing (NGS)-based genetic testing, particularly for newborn screening (NBS) clinical tests. The key objectives are to computationally design CRISPR guide RNAs and optimize depletion protocols to remove pseudogene-derived...

This Project Grant award from the National Institutes of Health (NIH) under the Research Infrastructure Programs (CFDA 93.351) aims to develop efficient methods for introducing small genetic changes, known as single nucleotide polymorphisms (SNPs) and variants of uncertain significance (VUS), into the zebrafish genome. The $181,754 award to Iowa State University, effective from September 15, 2024 to August 31, 2026, will leverage the university's "GenEweld" gene editing technology to...

This Project Grant award from the National Institutes of Health's Office of Research Infrastructure Programs (CFDA 93.351) provides $194,375 to the University of Wisconsin - Madison to develop improved genome editing tools for creating conditional knockout and amino acid change mutations in zebrafish. The key objectives are to: Establish a reliable method for generating floxed alleles in zebrafish using a synthetic exon approach, enabling conditional gene knockouts. Expand the synthetic exon...

This federal Project Grant award from the National Institutes of Health (NIH) Office of the Director (CFDA 93.351 - Research Infrastructure Programs) provides $217,533 to the University of Wisconsin System to develop a multi-color, multi-loci mouse reporter system for tracking hematopoietic stem cells (HSCs) and multipotent progenitor cells (MPPs) in their natural microenvironment. The key objectives are to: 1) Generate an HSC/MPP reporter mouse line by knocking in fluorescent protein...

This Project Grant award from the National Institutes of Health's Office of Research Infrastructure Programs (CFDA 93.351 - Research Infrastructure Programs) provides $844,954 to New York University School of Medicine to develop "genomically rewritten and tailored humanized mouse models for various organ disorders". The goal is to leverage advances in big DNA technology to produce mouse models that accurately reflect human disease susceptibility by inserting entire human genomic...