This $181,754 Project Grant awarded by the National Institutes of Health (NIH) Office of the Director (CFDA 93.351 - Research Infrastructure Programs) to Iowa State University of Science and Technology aims to develop efficient methods for introducing single nucleotide polymorphisms (SNPs) and variants of uncertain significance (VUS) into the zebrafish genome. The overarching goal is to create a gene editing approach, called GeneweldTM, that can efficiently target the insertion of SNPs and VUS into zebrafish for disease modeling and structure-function studies. The key proposed activities include testing GeneweldTM's ability to introduce SNPs at multiple loci, examining whether co-selection of an RFP reporter correlates with small genome changes, and enhancing SNP/VUS generation by overexpressing DNA repair enzymes. The award period runs from Sep 2024 to Aug 2026.
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