Project Grant R21DE035664

The National Institute of Dental and Craniofacial Research awarded The University of Texas Health Science Center at San Antonio $433,125 on August 1, 2026, under the Oral Diseases and Disorders Research program (CFDA 93.121) to investigate neuronal-glial coupling mechanisms in temporomandibular joint pain.

The project examines the role of connexin 43, a gap junction protein expressed in satellite glial cells, in modulating trigeminal ganglion neuron-glia communication and pain processing in vivo. The research uses in vivo voltage and calcium imaging combined with optogenetic glial manipulation to visualize and manipulate neuron-glia signaling networks in living animals. Specific Aim 1 characterizes in vivo trigeminal ganglion neuronal voltage dynamics in response to various sensory stimuli to investigate whether connexin 43 mediates neuron-glia signaling during temporomandibular joint pain. Specific Aim 2 establishes the causal role of satellite glial cell activity in driving maladaptive trigeminal ganglion neuronal activation using in vivo calcium and voltage imaging and selective optogenetic activation of satellite glial cells. The work addresses temporomandibular joint disorders, chronic orofacial pain conditions affecting 5–12 percent of adults and resulting in persistent jaw pain, jaw dysfunction, and reduced quality of life.

Performance occurs in San Antonio, Texas through July 31, 2028. The award is classified as a Project Grant, an assistance type supporting research and development activities.