This $588,426 Project Grant award, provided by the National Institutes of Health (NIH) Office of the Director under the Trans-NIH Research Support program (CFDA 93.310), aims to investigate how transcription factors efficiently locate and bind to specific DNA binding sites within the crowded nucleus of living cells. The project will employ advanced microscopy techniques to precisely track the 3D diffusion, DNA interactions, and target site discrimination of transcription factors involved in...
This National Science Foundation (NSF) Division of Chemical, Bioengineering, Environmental, and Transport Systems Project Grant of $500,000 awarded to Beth Israel Deaconess Medical Center, Inc. (Bidmc) aims to develop a novel, label-free method for dynamically sensing chromatin packing and structure in live cells. The 3-year project leverages the principles of fractal sensing and coherent confocal light absorption and scattering spectroscopic microscopy to create a 3D dynamic map of chromatin...
This $427,571 Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training Program (CFDA 93.859), supports research to uncover the molecular determinants of cellular identity and the role of chromatin dynamics in regulating gene expression across the cell cycle. The key products and services to be delivered include: Developing new time-resolved structural epigenomic approaches to identify chromatin states that cannot...
This $148,568 federal Project Grant awarded by the National Institute of General Medical Sciences (NIGMS) under CFDA 93.859 Biomedical Research and Research Training Program will support research to elucidate the role of chromatin structures in genomic imprinting. The project, led by Harvard University, aims to investigate the regulatory mechanisms and functional implications of allele-specific chromatin structures in the regulation of the MEST-COPG2 imprinted domain, which is associated with...
This federal Project Grant award from the National Institutes of Health (NIH) Office of the Director under the Trans-NIH Research Support program (CFDA 93.310) provides $418,749 to Yale University to conduct research on the spatial regulation of epigenetic memory. The project aims to investigate how dynamic chromatin organization regulates epigenetic memory during development, through a combination of highly multiplexed epigenetic state and chromatin imaging analysis in an organoid model, live...
This federal Project Grant award, provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training Program (CFDA 93.859), supports research aimed at understanding the mechanisms of heterochromatic transcriptional gene silencing. The $169,268 award to President and Fellows of Harvard College, doing business as Harvard Medical School, will fund investigations into how heterochromatin, a specialized chromatin structure, represses...
This $1,079,254 National Science Foundation project grant supports research investigating mechanisms of RNA polymerase II transcription and regulation using single molecule fluorescence techniques at the University of Colorado Boulder from March 2023 through February 2027. The research will study the assembly and disassembly of key protein complexes involved in early transcription stages using microscopy to observe individual molecules. Specifically, the research will investigate how the human...
This $292,727 Project Grant award from the National Institutes of Health (NIH) under the Trans-NIH Research Support program (CFDA 93.310) supports a research project by The Broad Institute, Inc. to discover novel regulators of 3D genome organization. The project aims to leverage large-scale genomic datasets to predict and experimentally validate new trans-acting factors that regulate chromatin interactions and genome architecture. By applying deep neural network models to in silico genetic...
This federal Project Grant award of $899,982 from the National Science Foundation's Biological Sciences program (CFDA 47.074) supports a collaborative research effort to study X-chromosome inactivation and genome organization in human cells. The research team from New York University, Harvard University, and the New Mexico Consortium will integrate advanced genomics data, computational modeling, and biophysical approaches to unravel the complex folding of the X-chromosome and its impact on...
The National Institute of General Medical Sciences (NIGMS) awarded a 5-year, $667,480 Project Grant (CFDA 93.859 - Biomedical Research and Research Training) to St. Jude Children's Research Hospital Inc. to conduct structural and functional studies of chromatin dynamics. The project aims to understand how chromatin structure and modifications regulate gene expression, DNA replication, repair, and cellular processes, with a focus on the biology of repetitive genomic elements and the mechanisms of...