This $930,000 Project Grant award from the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) is focused on advancing the understanding of transcription fidelity mechanisms in eukaryotic RNA polymerases. The project at the University of Alabama at Birmingham (UAB) will employ transient state kinetic approaches to quantify the differences in transcription and error correction mechanisms across the three eukaryotic RNA polymerases (I, II, and III). This research aims...
This project grant awarded by the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) supports fundamental research on the structural responses to chemical modifications that regulate gene expression in intrinsically disordered proteins. The $776,309 award to The Pennsylvania State University will implement new experimental methodologies for nuclear magnetic resonance (NMR) spectroscopy to characterize how phosphorylation and methylation of RNA polymerase II and...
This $900,000 project grant awarded by the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) supports research to understand the molecular mechanisms by which the DNA methyltransferase enzyme DNMT1 regulates gene silencing and the inheritance of cell identity. The project will utilize structural biology, biochemistry, and cell biology methods to investigate how DNMT1 activity is regulated by interactions with RNA and chromatin modifications. This work aims to...
The National Institute of General Medical Sciences (NIGMS) awarded a $451,802 Project Grant (CFDA 93.859 Biomedical Research and Research Training) to The Pennsylvania State University (Penn State) to conduct a structural study of the direct associations between cellular RNA polymerase and regulatory factors during the transcription cycle. The funding will support groundbreaking cryo-electron microscopy and single-molecule investigations to examine the heterogeneity and dynamics of RNA...
The National Science Foundation (NSF) awarded a $1,199,997 Project Grant under its Biological Sciences program (CFDA 47.074) to the University of Colorado-Denver. The grant will fund research to improve understanding of the mechanisms underlying messenger RNA (mRNA) localization and translation, and develop machine learning models to better predict protein production from mRNA. The project will apply recent advancements in RNA sequencing technology to study how factors like tRNA abundance and...
This federal Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) provides $1,215,158 to Cornell University to conduct research on the mechanisms underlying transcription factor sequence specificity. The project aims to apply high-throughput biochemical genomic assays and machine learning algorithms to identify the rules and mechanisms that govern protein binding to the human genome. Key research...
This Project Grant award from the National Institutes of Health (NIH) under the Trans-NIH Research Support program (CFDA 93.310) supports research to visualize the mechanisms at the intersection of chromatin, transcription, and epigenetics. The $1,525,500 award to Harvard Medical School aims to develop a combinatorial biochemical and structural approach called "Visual Biochemistry" that uses a fully reconstituted chromatin transcription system and time-resolved single-particle...
This $588,426 Project Grant award, provided by the National Institutes of Health (NIH) Office of the Director under the Trans-NIH Research Support program (CFDA 93.310), aims to investigate how transcription factors efficiently locate and bind to specific DNA binding sites within the crowded nucleus of living cells. The project will employ advanced microscopy techniques to precisely track the 3D diffusion, DNA interactions, and target site discrimination of transcription factors involved in...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) provides $391,250 over a 5-year period starting September 1, 2024 to support the University of Colorado in uncovering the mechanistic basis for the recruitment and activation of key chromatin modifier enzymes like Polycomb Repressive Complex 1 (PRC1), Polycomb Repressive Complex 2 (PRC2), and DNA Methyltransferase 1 (DNMT1). The research aims to...
The Project Grant award R35GM149520 in the amount of $775,170 from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) will support research to decode the regulatory mechanisms within the human genome. The project aims to better understand how transcription factors and other regulatory DNA features cooperatively control gene expression patterns during development and in response to various stimuli. Using advanced...