This National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) Project Grant award of $170,000 supports collaborative research between the Ellington and Adamala labs to engineer synthetic cells with expanded genetic alphabets and genetic codes. The key products and services to be delivered under this 3-year grant include:
Investigating the incorporation of non-canonical nucleotides into translation (Aim 1)
Incorporating non-canonical amino acids via non-canonical genetic...
This $1,120,000 Project Grant award from the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) aims to explore the boundaries of life's genetic alphabet beyond the natural 4-letter system (A, T, G, C). The research will develop model systems to study the biomolecular properties of a 6-letter genetic alphabet (A, T, G, C, P, Z) and map critical interactions necessary for translation of this expanded genetic code. The project will use synthetic biology and chemical...
The National Science Foundation Division of Molecular and Cellular Biosciences awarded $1,299,732 to the University of Texas at Austin under a Project Grant to support research titled "COLLABORATIVE RESEARCH: SYNTHETIC AND SYSTEMS BIOLOGY APPROACHES TO SEMI-SYNTHETIC CELLS WITH EXPANDED DNA ALPHABETS." The three-year award beginning August 15, 2021 falls under the Biological Sciences program (CFDA 47.074) to promote progress in the biological sciences and strengthen the Nation's...
The Foundation For Applied Molecular Evolution, Inc. was awarded a $1.2 million Project Grant from the National Science Foundation Division of Molecular and Cellular Biosciences to support research titled "COLLABORATIVE RESEARCH: SYNTHETIC AND SYSTEMS BIOLOGY APPROACHES TO SEMI-SYNTHETIC CELLS WITH EXPANDED DNA ALPHABETS" from August 15, 2021 through July 31, 2024.
The grant supports research under the NSF's Biological Sciences program (CFDA 47.074), which aims to advance...
This National Science Foundation (NSF) federal Project Grant award under the Biological Sciences program (CFDA 47.074) is funding the University of Illinois to construct a new type of self-replicating synthetic cell system. The $448,355 award from June 1, 2024 to May 31, 2027 supports the development of engineered synthetic cells that can grow, divide, and replicate their genetic information using nucleic acids as both structural and genetic components. The project aims to investigate the...
This $320,861 federal Project Grant award from the National Science Foundation (NSF) under the Biological Sciences program (CFDA 47.074) supports research to develop tools and methodologies for reading expanded genetic alphabets with bases beyond the standard A, T, G, and C. The key objectives are to: 1) improve the accuracy and generalizability of next-generation sequencing for unnatural base pairs using deep learning; 2) develop single-context sequencing models to measure polymerase...
The National Science Foundation (NSF) awarded a $1,000,000 Project Grant under the Biological Sciences (CFDA 47.074) program to The Regents of the University of California, San Francisco (UCSF) to design, build, and test synthetic signaling systems built from computationally designed de novo protein components. The goal is to develop synthetic membrane signaling systems capable of programmable receptor clustering, tunable signal amplification, and bidirectional transmembrane signaling. This...
The National Science Foundation Division of Molecular and Cellular Biosciences awarded Rice University a $373,174 Project Grant under the Biological Sciences federal grant program (CFDA 47.074) to conduct research toward developing lifelike synthetic cells through engineered control of DNA replication from August 15, 2021 to July 31, 2024. The Biological Sciences program aims to advance biological knowledge and understanding of major problems to strengthen the nation's scientific enterprise....
The National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) awarded a $1,000,000 Project Grant to the University of California, Berkeley to develop a polyketide-based platform to produce a variety of non-canonical amino acids (NCAAs) and demonstrate their incorporation into proteins expressed by Bacillus subtilis. The project aims to expand the set of amino acids beyond the 20 naturally occurring ones, which would enable the production of proteins with enhanced properties...
This $3 million National Science Foundation project grant supports research at the University of Texas at Austin to develop synthetic adhesive cells for controlled intracellular delivery. Funded under the Biological Sciences program (CFDA 47.074), the three-year award will engineer synthetic cells with user-defined control over molecular transfer between synthetic and live cells. Researchers will build on prior work developing synthetic cells that form adhesive junctions and transfer cargo...
This $300,000 Project Grant awarded by the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) supports collaborative research at the University of Texas at Austin to develop synthetic cells with expanded genetic alphabets and genetic codes. The key objectives are to:
Investigate the incorporation of non-canonical nucleotides into protein translation systems (Aim 1),
Incorporate non-canonical amino acids using non-canonical genetic alphabets (Aim 2), and
Use artificial evolution to optimize translation systems with non-canonical components (Aim 3).
The overarching goal is to transcend the evolutionary constraints of natural cells and create life-like systems with expanded chemistries, potentially leading to the incorporation of over 24 novel amino acids into proteins. This 3-year project aims to advance the state-of-the-art in synthetic biology by engineering synthetic cells with expanded genetic codes. No sub-awards are planned under this grant.