This $320,861 federal Project Grant awarded by the National Science Foundation (NSF) Division of Molecular and Cellular Biosciences under CFDA 47.074 Biological Sciences supports research to develop tools for reading expanded genetic alphabets containing bases beyond A, T, G, and C. The project aims to improve the accuracy and generalizability of next-generation DNA sequencing for these unnatural base pairs through deep learning. It also develops new methods to measure polymerase replication fidelity for unnatural bases in systems like PCR and LAMP. Additionally, the project investigates the biocompatibility of unnatural base pairs in microbial hosts by examining metabolic processes, replication fidelity, error repair pathways, and host responses. This research has the potential to enable transformative applications in nucleic acid biotechnology, including improving diagnostic assays, discovering novel therapeutics, and enhancing biomanufacturing techniques. The award period is from Sep 1, 2025 to Aug 31, 2030.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $320.9k | 1/15/25 |