Total Tissue Sample RNA Isolation, mRNA Library Preparation, Sequencing, and Bioinformatic Processing/Analysis

The Food and Drug Administration's National Center for Toxicological Research seeks total tissue sample RNA isolation, mRNA library preparation, sequencing, and bioinformatic processing and analysis, with quotes due August 10, 2026 at 1:00 PM Central Time. This is an unrestricted full and open competition with no set-aside.

Place of performance is Jefferson, Arkansas 72079. The requirement covers 256 rat brain tissue samples across four independent brain regions, each with control and three dose levels across eight male and eight female subjects. Minimum specifications include total RNA extraction with quality control, mRNA library preparation using PolyA enrichment or comparable strategy, sequencing at 15–30 million paired-end 50–150 bp reads, and comprehensive bioinformatic analysis including quality control, genome alignment, differential expression analysis, and functional enrichment analysis (GO, KEGG, GSEA). Results must be delivered via email as Excel or delimited text files within six months of sample receipt. The government will provide tissue samples no later than September 30, 2026.

The contract is Firm-Fixed-Price with FOB Destination to Jefferson, Arkansas 72079. Payment terms are Net 30 Days after government acceptance. The NAICS code is 541380, Testing Laboratories and Services; PSC is Q301, Reference Laboratory Testing. All quotes must include a Unique Entity Identifier from SAM registration and technical information demonstrating compliance with all requirements. The government intends to award to the lowest price technically acceptable and responsible offeror. Invoices must be submitted electronically through the Department of Treasury's Invoice Processing Platform. Contact howard.nesmith@fda.hhs.gov with questions.