Sol_75N98026Q00477.pdf

REQUEST FOR QUOTATION

(THIS IS NOT AN ORDER)

THIS RFQ IS IS NOT A SMALL BUSINESS SET-ASIDE

15. DATE OF QUOTATION

16. SIGNER

a. NAME (Type or print)

c. TITLE (Type or print)

b. TELEPHONE

AREA CODE

NUMBER

STANDARD FORM 18 (REV. 6/1995)

Prescribed by GSA-FAR (48 CFR) 53.215-1(a)

AUTHORIZED FOR LOCAL REPRODUCTION

Previous edition not usable

8. TO:

b. COMPANYa. NAME

c. STREET ADDRESS

d. CITY e. STATE f. ZIP CODE

9. DESTINATION

a. NAME OF CONSIGNEE

b. STREET ADDRESS

d. STATE e. ZIP CODE

7. DELIVERY

FOB DESTINATION

OTHER

(See Schedule)

10. PLEASE FURNISH QUOTATIONS TO THE

ISSUING OFFICE IN BLOCK 5a ON OR BEFORE CLOSE OF BUSINESS (Date)

IMPORTANT: This is a request for information and quotations furnished are not offers. If you are unable to quote, please so indicate on this form and return it to the address in Block 5a. This request does not commit the Government to pay any costs incurred in the preparation of the submission of this quotation or to contract for supplies or service. Supplies are of domestic origin unless otherwise indicated by quoter. Any representations and/or certifications attached to this Request for Quotation must be completed by the quoter.

11. SCHEDULE (Include applicable Federal, State and local taxes)

ITEM NUMBER

(a)

SUPPLIES/SERVICES

(b)

QUANTITY

(c)

UNIT

(d)

UNIT PRICE

(e)

AMOUNT

(f)

12. DISCOUNT FOR PROMPT PAYMENT

a. 10 CALENDAR DAYS (%) b. 20 CALENDAR DAYS (%) c. 30 CALENDAR DAYS (%) d. CALENDAR DAYS

NUMBER PERCENTAGE

NOTE: Additional provisions and representations are are not attached.

13. NAME AND ADDRESS OF QUOTER

a. NAME OF QUOTER

b. STREET ADDRESS

c. COUNTY

d. CITY e. STATE f. ZIP CODE

14. SIGNATURE OF PERSON AUTHORIZED TO

SIGN QUOTATION

PAGE OF PAGES

1. REQUEST NUMBER 2. DATE ISSUED 3. REQUISITION/PURCHASE REQUEST NUMBER 4. CERT. FOR NAT. DEF.

UNDER BDSA REG. 2

AND/OR DMS REG. 1

RATING

5a. ISSUED BY 6. DELIVER BY (Date)

NAME TELEPHONE NUMBER

AREA CODE NUMBER

c. CITY

5b. FOR INFORMATION CALL (NO COLLECT CALLS)

National Institutes of Health OD - Office of Logistics and Acquisition Operations 6100 Executive Blvd., Room 6B05 Bethesda, MD 20892-7511

75N98026Q00477

NICHOLAS CAPOBIANCO

+10

000-000000

05/29/2026

06/08/2026 0900 ED

Multiple

The purpose of this NON-COMPETITIVE

COMBINED SYNOPSIS / SOLICITATION is to acquire Oxford Nanopore PromethION Flow

Cells and cDNA-PCR Sequencing Kits in

Support of the Center for Alzheimer's

Disease and Related Dementias (CARD).

Period of Performance: 06/09/2026 to

12/09/2026

The following FAR clauses and Provisions are applicable to this award.

FAR 52.204-13 System for Award

Management Maintenance (Oct 2018);

FAR 52.204-18 Commercial and Government

Continued...

1 7

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGES

NAME OF OFFEROR OR CONTRACTOR

SUPPLIES/SERVICES

(B)

UNIT

(D)

UNIT PRICE

(E)

AMOUNT

(F)

OPTIONAL FORM 336 (4-86)

Sponsored by GSA FAR (48 CFR) 53.110

ITEM NO.

(A)

QUANTITY

(C)

NSN 7540-01-152-8067

75N98026Q00477

Entity Code Maintenance (Aug 2020);

HHSAR 352.222-70, Contractor Cooperation in

Equal Employment Opportunity Investigations

(Dec 2015).

FAR 52.212-4 Contract Terms and Conditions -In addition to all the applicable commercial

Products and Commercial Services (Nov 2023)

FAR 52.212-5 Contract Terms and Conditions

Required to Implement Statutes or Executive

Orders/Commercial Products and Commercial

Services (Jan 2025)

FAR 52.252-2 CLAUSES INCORPORATED BY REFERENCE

To avoid delay in payment, all invoices must be uploaded into the invoicing processing platform

(IPP) (https://www.ipp.gov). The invoice must match the line item description on the purchase order. It must also state that payment is to be made using a three-way match.

NON-COMPETITIVE

COMBINED SYNOPSIS / SOLICITATION

Title: Oxford Nanopore PromethION Flow Cells and cDNA-PCR Sequencing Kits in Support of the Center for Alzheimer's Disease and Related Dementias (CARD)

(i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested, and a separate written solicitation will not be issued.

(ii) The solicitation number is 75N98026Q00477. This solicitation is issued as a Notice of Intent (NOI) for a non-competitive combined synopsis/solicitation to award a contract or purchase order without providing for full and open competition (brand-name).

This acquisition is conducted under the authority of FAR 12.603, Streamlined Solicitation for Commercial Products or Commercial Services, and is not expected to exceed the simplified acquisition threshold. Additional authority includes 41 U.S.C. 1901 and FAR 6.302-3(a)(2)(ii).

The National Institutes of Health (NIH), Office of the Director, Office of Acquisitions, intends to negotiate and award a Firm-Fixed-Price purchase order on a non-competitive basis to Oxford Nanopore Technologies Inc., One Kendall Square, Building 1400, Suite 14-305, Cambridge, MA 02139 for PromethION flow cells and cDNA-PCR sequencing kits required to support the National Institute on Aging's Center for Alzheimer's Disease and Related Dementias (CARD) long-read RNA sequencing project on ROSMAP brain samples. Oxford Nanopore Technologies Inc. is the sole manufacturer of the proprietary flow cells (FLO-PRO114M) and cDNA-PCR Sequencing Kit V14 (SQK-LSK114-XL) required to operate CARD's existing PromethION 48 sequencing infrastructure and to enable direct matched comparison with previously generated long-read whole-genome sequencing data on the same ROSMAP samples.

(iii) The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2026-01, dated March 13, 2026.

(iv) The associated NAICS code is 325413 – In-Vitro Diagnostic Substance Manufacturing, and the small business size standard is 1,250 employees. This requirement is not set aside for small business.

(v) Description of Requirement:

The Contractor shall provide the following Oxford Nanopore brand-name reagents and consumables in support of long-read RNA sequencing of 250 frontal cortex brain samples from the Religious Orders Study and Memory and Aging Project (ROSMAP) for the identification of transcript-level differences associated with Alzheimer's disease:

CLIN Description Catalogue Number Quantity

0001 PromethION Flow Cells Packs DNA FLO-PRO114M 72

0002 cDNA-PCR Sequencing Kit V14 SQK-LSK114-XL 48

All deliverables required under this contract shall be packaged, marked, and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Flow Cell salient characteristics:

• Must be able to generate consensus data at a modal accuracy above 99%.

• Must be compatible with Nanopore PromethION 48 sequencers in form, fit, and function.

• Must be compatible with existing CARD PromethION 48 sequencer infrastructure and established CARD bioinformatics pipelines for the ROSMAP long-read WGS dataset.

cDNA-PCR Sequencing Kit salient characteristics:

• Must provide a cDNA-PCR Sequencing Kit to prepare cDNA for nanopore sequencing.

• Must require no more than 10 ng poly(A)+ RNA or 500 ng total RNA as starting material.

• Must provide for 6 reactions.

• Must be compatible with R10.4.1 nanopore chemistry used in CARD's existing

PromethION workflows.

• Must enable direct comparison with previously generated long-read WGS data on matched ROSMAP samples.

Period of Performance: 6 months from date of award.

(vi) Delivery Location:

National Institute on Aging Center for Alzheimer's Disease and Related Dementias 9000 Rockville Pike

(vii) [Reserved]

(viii) The provision at FAR 52.212-1, Instructions to Offerors—Commercial Items, applies to this acquisition.

(ix) The provision at FAR 52.212-2, Evaluation—Commercial Items, applies to this acquisition.

(a) A written notice of award or acceptance of an offer, issued to the successful offeror within the time specified in the offer, shall result in a binding contract without further action by either party.

The Government reserves the right to accept any offer (or part of an offer) before the expiration date, whether or not discussions are conducted, unless a written notice of withdrawal is received prior to award.

(x) Offerors shall include a completed copy of FAR 52.212-3, Offeror Representations and Certifications—Commercial Items, with their offer.

(xi) FAR 52.212-4, Contract Terms and Conditions—Commercial Items, applies to this acquisition.

(xii) FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders—Commercial Items, applies to this acquisition.

(xiii) Additional contract requirements or terms and conditions, as determined necessary by the Contracting Officer and consistent with customary commercial practices: None.

(xiv) The Defense Priorities and Allocations System (DPAS) is not applicable to this requirement.

(xv) Submission Requirements:

Responses must include sufficient information to demonstrate the respondent's capability to provide the required product or service. Price quotations shall include, at a minimum: unit price, list price, shipping and handling costs, delivery timeframe after award, delivery terms, prompt payment discount terms, F.O.B. point (destination or origin), product or catalog numbers, product descriptions, and any other relevant information.

Evaluation factors may include, but are not limited to: past performance, technical capability, special features, trade-in considerations, expected lifecycle, warranty, maintenance availability, and environmental or energy efficiency considerations.

Respondents claiming to be manufacturers or authorized resellers of a specified brand-name product must provide:

(a) Product, catalog, model, and/or part number(s); (b) Product description; (c) Documentation demonstrating that the offered item meets all required salient characteristics; (d) Quantity; (e) Estimated price or cost; (f) Shipping, handling, and/or installation charges; and (g) Delivery timeframe after receipt of order.

Responses must also include the Unique Entity Identifier (UEI), Taxpayer Identification Number (TIN), and business size certification. All offerors must be actively registered in the System for Award Management (SAM) at www.sam.gov.

All responses must be received by June 8, 2026, at 9:00 AM EDT and must reference solicitation number 75N98026Q00477. Responses may be submitted electronically to nicholas.capobianco@nih.gov.

mailto:nicholas.capobianco@nih.gov

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The purpose of this acquisition is to provide supplies to the Center for Alzheimer’s Disease and Related Dementias for neurodegenerative disease research for the purpose of long read sequencing on Oxford Nanopore manufactured sequencing systems. These reagents will be used to identify causative genes for neurodegenerative diseases.

2. Background Information and Objective:

Building on National Institute on Aging’s Center for Alzheimer’s Disease and Related Dementias (CARD) existing long-read whole-genome sequencing (WGS) data for The Religious Orders Study and Memory and Aging Project (ROSMAP) brain samples, the lab is extending this work to generate matched long-read RNA sequencing from 250 frontal cortex brain samples to identify transcript-level differences associated with Alzheimer’s disease(AD). The availability of matched long-read genomic, epigenetic, and transcriptomic data from the same individuals provides a uniquely powerful framework to interrogate the genetic, epigenetic, and transcriptional mechanisms underlying AD.

Isoform-resolved transcriptomic profiles will enable detection of disease-associated splicing, expression, and RNA modification changes that are not accessible with short-read approaches. Integrated with the rich pathological and clinical annotations available for ROSMAP, this project will create a high-value, multi-omic long-read resource for the community and establish a scalable model for comprehensive molecular profiling in neurodegenerative disease.

3. Generic Name of Product: cDNA Sequencing kits

4. Purchase Description:

Contract Line Item Description Catalogue Number Quantity PromethION Flow Cells Packs DNA FLO-PRO114M 72.00 cDNA-PCR Sequencing Kit V14 SQK-LSK114-XL 48.00

Salient characteristics All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Flow Cell salient characteristics:

Must be able to generate consensus data at a modal accuracy above 99% Must be compatible with Nanopore PromethION 48 sequencers in form, fit, and function.

Must be compatible with existing CARD PromethION 48 sequencer infrastructure and established CARD bioinformatics pipelines for the ROSMAP long-read WGS dataset.

cDNA-PCR Sequencing Kit salient characteristics:

Must provide cDNA-PCR Sequencing Kit to prepare cDNA for nanopore sequencing.

Must require no more than 10 ng poly(A)+ RNA or 500 ng total RNA as starting material.

Must provide for 6 reactions.

Must be compatible with R10.4.1 nanopore chemistry used in CARD's existing PromethION workflows" and "Must enable direct comparison with previously generated long-read WGS data on matched ROSMAP samples.

5. Quantity:

Contract Line Item Description Catalogue Number Quantity PromethION Flow Cells Packs DNA FLO-PRO114M 72 cDNA-PCR Sequencing Kit V14 SQK-LSK114-XL 48

6. Period of Performance: 6 Months from Date of Award

7. Option(s): Not applicable.

Title