Attachment 23 Laboratory SOPs.pdf

CDP COBRATF Training Operations and Support Services

Attachment 23

Laboratory SOPs i

STANDARD OPERATING PROCEDURE

Organization: Center for Domestic Preparedness Date: 8/25/2017 Title: Agent Laboratory Procedures

CDP-COBRATF-CHEM SOP 001.2

I. Purpose To ensure all personnel know appropriate action, safety measures and general operating procedures used while working with neat chemical agent in the agent laboratory

II. Supersession This SOP supersedes:

CDP COBRATF CHEM SOP 001.0, Agent Lab Procedures, Effective Date September 8, III. Authority CDP COBRATF Chemical Surety Program Standards Document

IV. References A. CDP COBRATF Toxic Agent Exposure Control Plan

B. CDP COBRATF Incident Management Manual (IMM)

C. CDP COBRATF Chemical Hygiene Plan

D. CDP COBRATF Laboratory Training Plan

E. CDP COBRATF TDS SOP 001, Training Day Support Activities

F. CDP COBRATF AMP SOP 002, MINICAMS Method Procedures

G. CDP COBRATF CHEM WI 001, Preparation of 10% NaOH (Sodium Hydroxide, Caustic Soda)

A. Memo for Record, Subject: Maximum Allowable Amount of Chemical Agent Accountable without Causative Research

B. Department of Defense Form 1911, Materiel Courier Receipt

C. CQATFM-SR-1, CASARMS Quality Assurance Team Form SR-1

V. Acronyms and Definitions A. AD – Assistant Director B. AMC – Army Materiel Command C. Ampoules – A glass container or vial used as the primary container to hold chemical agent Material D. APR – Full Face NIOSH-certified Air Purifying Respirator file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/CSPSD%20August%2015,%202014.pdf file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/CDP%20Safety%20Plan%20Annexes/Annex%20M%20Toxice%20Agent%20Exposure%20Plan.pdf file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/IMM,%20Revision%201.pdf file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/CDP%20Safety%20Plan%20Annexes/Annex%20L%20Chemical%20Hygine%20Plan.pdf file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/Laboratory%20Training%20Plan,%20Revision%200.pdf file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/COBRATF%20SOPs/TDS%20SOPs/TDS%20SOP%20001%20Training%20Day%20Support%20Activities/TDS%20001%20Training%20Day%20Support%20Activities.pdf file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/COBRATF%20SOPs/AMP%20SOP,%20WIs/AMP%20SOP%20002.1%20MINICAMS%20Methods%20Procedures/AMP%20SOP%20002.1%20MINICAMS%20Procedures.pdf file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/COBRATF%20SOPs/CHEM%20SOPs/CHEM%20WI%20001.0%20Prep%20of%2010%25%20NaOH.pdf portuesep Typewritten Text portuesep Typewritten Text portuesep Typewritten Text portuesep Typewritten Text portuesep Typewritten Text

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E. CCTV – Closed Circuit Television F. CERP – Contractor/Employee Reliability Program G. CSR – Custodial Stock Record; form used during receipt of agent H. DD or DoD – Department of Defense I. ECBC – Edgewood Chemical Biological Center J. Exothermic Reaction – A chemical reaction that produces heat as a by-product

D. g – grams; a metric unit of mass

E. GB – Sarin; O-isopropyl methylphosphonofluoridate; a non-persistent nerve agent

F. Hot Trash – All refuse accumulated during dilute standard preparation

G. HTH – High Test Hypochlorite; calcium hypochlorite

H. IAW – In Accordance With

I. IDLH – Immediately Dangerous to Life and Health

J. IH – Industrial Hygiene or Industrial Hygienist

K. LSC – Laboratory Shipping Container

L. MAUR – Monthly Agent Utilization Report

M. MINICAMS – Miniature Continuous Air Monitoring System

N. mL – milliliter; 1/1000 L; a metric unit of measure

O. Mailing Tube – A spun fiberboard tube with a metal screw top used to hold and protect the ampoule

P. M8 paper – Specially formulated paper to detect presence of nerve agents via color change reaction

Q. NaOH – Sodium hydroxide; caustic soda; a decon material

R. Neat Chemical Agent Material – A chemical agent not mixed or diluted

S. NFPA – National Fire Protection Agency

T. NIOSH – National Institute for Occupational Safety and Health

U. PPE – Personnel Protective Equipment

V. pH – The symbol for the logarithm of the reciprocal of hydrogen ion concentration in gram atoms per liter, used to express the acidity or alkalinity of a solution on a scale of 0 to 14, where less than 7 represents acidity, 7 neutrality, and more than 7 alkalinity

W. RDECOM – Research Development and Engineering Command

X. RDT&E – Research Development Test and Evaluation

Y. STEL – Short Term Exposure Limit

Z. TAP – Toxicological Agent Protective portuesep Typewritten Text portuesep iii

AA. TOS – Training Operations Specialist

AB. VX – O-ethyl-S-(2-diisopropylaminoethyl) methylphosphonothiolate; a persistent nerve agent

VI. Responsible Office Agent Laboratory portuesep iv

Table of Contents I. Purpose ...................................................................................................................................... i II. Supersession .............................................................................................................................. i III. Authority .................................................................................................................................... i IV. References.................................................................................................................................. i V. Acronyms and Definitions ......................................................................................................... i VI. Responsible Office................................................................................................................... iii VII. Standard Operating Procedures

A. Safety and Housekeeping

B. Personnel Training

C. Visitors to the Neat Agent Laboratory

D. Emergency Situations

E. Ventilation/Hood System

F. Agent Monitoring

G. Personnel Protective Equipment

H. Gloves:

I. Emergency Shower and Eye Wash:

J. Broken Glass Disposal:

K. Equipment used in agent analysis must be deconned immediately upon completion of analysis..………………………………………………………………………………………..6

L. NEVER flush any agent contaminated waste down the drain

M. Wash hands prior to exiting the lab

N. Certification of Agent Custodians

O. Receipt of Neat Chemical Agent Material

P. Accountability

Q. Syringe Operations in the Glove Box

R. Preparation of Decontaminant and Neat Agent Destruction (Amount > 1 gram)

VIII. Forms Prescribed

Chart 2

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VII. Standard Operating Procedures

NOTE: See attached hazard assessment (Appendix A) A. Safety and Housekeeping

1. Labortory Entrance

NOTE: No eating, drinking, chewing, or smoking is permitted in the laboratory.

a. Entry to the laboratory is restricted to authorized personnel. The laboratory is locked when unoccupied.

b. A sign is posted on the laboratory door that warns personnel of the presence of VX and GB and lists entrance requirements.

c. Any real or suspected agent exposure must be reported to supervisory personnel immediately. Halt any operation when abnormal or hazardous conditions occur.

d. Perform pre/post operational checks on a daily basis and record on CDP COBRATF AMPF 050, Chemical Laboratory Operational Checklist.

e. Any open sores or wounds must be evaluated by the medical staff prior to working in the laboratory.

B. Personnel Training

1. Personnel working with agents are trained in the use and handling prior to assignment.

This training includes:

a. How to don, wear, and remove protective clothing

b. The use of decontaminating materials

c. The procedures for spill clean-up and personnel exposure

2. Personnel receive initial training and are tested annually on signs and symptoms of agent poisoning.

3. All personnel working in the neat agent laboratory must read and document training on this SOP.

C. Visitors to the Neat Agent Laboratory Visitors to the laboratory are given a briefing on laboratory safety procedures.

D. Emergency Situations Respond IAW IMM.

Intentionally left blank.

file://amcd12f01/forms/CDP%20COBRATF%20FORMS/AMP%20Forms/AMPF%20050.0%20Chemical%20Laboratory%20Operationnal%20Checklist.docx file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/IMM,%20Revision%201.pdf portuesep Typewritten Text portuesep

E. Ventilation/Hood System

NOTE 1: When surveyed by IH, all hoods must maintain an inward airflow as verified by smoke tests or other visual means IAW the TA-ECP.

1. Hood face velocity surveys are performed by an IH technician/officer on a quarterly basis and when the system has undergone major repairs.

2. The hood is provided with both visible and audible alarm devices that are activated when the velocity reading is outside the set range. The visible alarm is located where personnel, while working at the hood, can readily see it.

3. Hood procedures:

a. Hood alarms are checked daily and recorded on CHEMF 002. If the alarms are not functioning properly, O & M personnel are notified.

b. The average daily reading (100±20 1fpm) is recorded on CHEMF 002.

c. Safety control is notified if the average face velocity is not within range. The hood is not used until operating parameters are re-establised.

d. Only the ventilation hood face is opened while working with agent or other chemicals.

e. The hood is closed at the end of the operation and/or prior to exiting the laboratory.

f. No agent or agent-contaminated equipment is placed within twenty (20) centimeters of the face of the hood. This is designated by a taped line on the hood floor.

4. The filtration/ventilation/hood system in the laboratory remains on at all times.

NOTE 2: Glovebox operations are monitored via CCTV and security perimeter is maintained.

5. In case of ventilation failure, stop work, close hood and evacuate the lab.

file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20002.2%20Neat%20Agent%20Lab%20Pre%20Post%20Operational%20Checklist.docx file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20002.2%20Neat%20Agent%20Lab%20Pre%20Post%20Operational%20Checklist.docx portuesep Typewritten Text portuesep

F. Agent Monitoring

1. Monitoring of the lab air is accomplished on a continuous basis during hot operations by the MINICAMS at the STEL and IDLH levels.

2. First entry monitoring, protective clothing, and possible decontamination is required under the following conditions:

a. Agent spill outside containment

b. Major agent spill (>20mL) within the hood

c. Ventilation failure of a hood with uncontained agent

d. Ventilation failure of hood with contained agent lasting longer than 24 hours

e. IDLH MINICAMS alarm in the lab.

3. Prior to re-entry, a deliberate response plan will be developed based on known operational information. This plan shall be approved by the AD.

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G. Personnel Protective Equipment

See hazard assessment in Appendix A for required PPE.

H. Gloves:

1. Type of gloves used:

a. Chemical protective butyl rubber gloves (standard):

(1) Wear when handling neat agent contaminated or potentially neat agent contaminated items.

(2) Wear during emergency situations in the handling of agent.

(3) Wear to clean up spills outside the hood or glove box.

(4) Wear as covers over glove box gloves.

b. Use 15 mil butyl rubber gloves (non-standard) for all glove box operations.

c. Nitrile gloves:

(1) Wear when working in the glove box (under glove box gloves), and while working in the vault hood (under butyl gloves).

(2) Change gloves when torn, contaminated, or as necessary for operation being performed.

(3) Discard gloves worn while performing hot operations in the hot trash container, located in the vault hood, upon completion of operation.

(4) Bag hot trash after completion of agent operations and transfer to pass-thru chute for disposal.

2. Changing gloves:

a. Standard Chemical Protective Gloves

(1) Replace these gloves at least once a month.

(2) Place a pair of gloves in the front of each hood.

(3) Consolidate, bag, and place old gloves in the pass-thru chute for PC&E cleaning and re-certification.

(4) Record glove change out on CHEMF 001.

(5) Gloves contaminated with liquid neat agent are deconned and discarded with the hot trash for treatment and disposal.

b. Glove Box Gloves

NOTE: This is a two-person operation. DO NOT perform this task alone.

(1) Change out glove box gloves and butyl rubber gloves in the hoods with a clean, certified pair of gloves immediately following neat agent operations.

file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20001.0%20Glove%20Filter%20Changing%20Schedule.doc

(2) Person 1: Remove the O-rings and roll the edge of the glove box into the grove of the O-ring that was closest to the edge.

(3) Person 2: Place the new glove on your arm and roll the top of the glove a few inches. Place the rings from the glove box adapter up the arm of the donned glove box glove.

(4) Place your arm with the new glove inside the old glove.

(5) Person 1: Ensuring that the glove is positioned straight, and not twisted;

place the end of the new glove over the old glove, around the glove box adapter, and extended dto the box seal.

(6) Persons 1 & 2: Hold the new glove in place while Person 1 seals the glove around the adapter by placing the two O-rings in the grove closest to the box.

(7) Person 2 removes the old glove while Person 1 seals the glove around the adapter, behind the rings.

NOTE: Person 2 will hold the adapter gasket on with one hand (outside the glove box).

The gasket will come off into the removed glove if it is not held in place during the removal.

(8) Person 2: While arm is still in new glove replace both O-rings.

(9) Repeat steps (1) through (7) above for the other glove.

(10) Person 1: Replace both o-rings.

(11) Repeat steps (1) through (9) above for the other glove.

(12) Bag and tag the old glove box gloves and place in the pass-thru chute for cleaning and recertification by PC&E.

(13) Change out glove box gloves and butyl rubber gloves in the hoods with a clean, certified pair of gloves immediately following neat agent operations.

(14) Record the change dates on CHEMF 001.

file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20001.0%20Glove%20Filter%20Changing%20Schedule.doc

I. Emergency Shower and Eye Wash:

1. Check shower and eye wash each day operations are performed.

2. Record checks on CHEMF 002.

J. Broken Glass Disposal:

1. Place chipped, cracked, or broken glassware in a hot trash container with a lid and appropriate decon solution for decontamination and disposal.

2. Do not pick up pieces of broken glass with butyl gloves. This ensures butyl is not accidentally cut. If you must pick up pieces of broken glass, use caution.

3. Use tongs to pick up large pieces and paper towels for smaller pieces.

K. Equipment used in agent analysis must be deconned immediately upon completion of analysis. Equipment includes, but is not limited to, glassware, tongs, forceps, and syringes.

L. NEVER flush any agent contaminated waste down the drain.

M. Wash hands prior to exiting the lab.

file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20002.2%20Neat%20Agent%20Lab%20Pre%20Post%20Operational%20Checklist.docx

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N. Certification of Agent Custodians

1. Certification Process

a. All personnel required to become Agent Custodians must read and understand section B of this SOP.

b. Individuals sign and date appropriate training forms.

c. All required training is annotated on CHEMF 008.

NOTE 1: All proposed Agent Custodians are certified by the Accountable Officer and the IH, then recommended to the AD for appointment.

d. When the Chemist or the TOS supervisor, as appropriate, determines that the proposed Agent Custodian has been trained in their respective procedures, the Accountable Officer is notified.

e. Once notified, the Accountable Officer schedules a certification date for the proposed Agent Custodian who will be certified by the Accountable Officer and

IH.

f. The certification process consists of all normal neat agent handling procedures with the following exceptions:

(1) Water is used in the certification procedure in lieu of neat agent.

(2) Students are not present at the location where the certification and validation process is being conducted.

(3) The individual must be fully visible to the Accountable Officer and the IH during the entire certification process so they may record approval for each item performed on CHEMF 008.

g. Upon satisfactory completion of the certification process, the Accountable Officer and IH inform the AD via copies of CHEMF 008 recommending or denying the potential Agent Custodian.

h. The AD submits a duty appointment letter to the Director of Training and Education for approval.

i. Individuals not completing the certification process satisfactorily are required to undergo additional training before re-taking the certification test.

2. Validation Process

NOTE 2: All validations for proposed Agent Custodians must be viewed by the Accountable Officer and the IH.

a. Upon receipt of the duty appointment letter, the Chemist, or the TOS supervisor, notifies the Accountable Officer and requests a validation date.

b. To validate each Agent Custodian, the Accountable Officer, and the IH observe the Agent Custodian in the handling of neat chemical agent in the toxic agent training area or agent laboratory.

file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20008.%202%20Agent%20Custodians%20Certification%20for%20Agent%20Pouring.docx file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20008.%202%20Agent%20Custodians%20Certification%20for%20Agent%20Pouring.docx file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20008.%202%20Agent%20Custodians%20Certification%20for%20Agent%20Pouring.docx portuesep Typewritten Text portuesep

c. Upon satisfactory completion of the validation testing, the Accountable Officer and the IH complete the appropriate memo giving their approval of the validation of the individual as an Agent Custodian.

d. Individuals not completing the validation testing satisfactorily are required to undergo additional training and must recertify prior to validation.

3. All documentation certifying and validating an individual as an Agent Custodian is placed in the individual’s training file.

4. If an individual has not handled agent within a 12 month time period, he/she must be re-certified prior to handling agent.

5. If an individual has not handled agent within a 6 month time period, he/she must be re-validated prior to handling agent as part of their agent custodian duties.

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O. Receipt of Neat Chemical Agent Material

1. Preparation for Receipt of Neat Chemical Agent Material

Note 1: All personnel supporting this operation in the agent laboratory (with the exception of agent laboratory personnel) must be approved by the AD to perform the neat agent receiving tasks below. If approved, the personnel must read, understand and sign this SOP before entering the agent laboratory to receive neat chemical agent.

a. Government staff:

(1) Are notified of the arrival date of agent on an as needed basis.

(2) Verify receiving date with the Accountable Officer.

b. Appropriate Contractor Project Manager:

(1) Is notified of the scheduled arrival date by the Accountable Officer.

(2) Notifies the appropriate personnel of the arrival date.

(3) Supply DoD with personnel authorized to accept shipment, when requested.

(4) Gives any necessary supplemental instructions to the MINICAMS laboratory, support personnel and safety control.

c. Agent laboratory staff ensure all appropriate personnel have received proper training to open LSC and remove agent prior to shipment delivery.

(1) Practice with the example LSC.

(2) See supply technician to retrieve example LSC from storage area.

2. Agent laboratory personnel verify with safety control that all necessary personnel are in place before neat agent arrives on site.

3. Agent laboratory duties for preparing laboratory for receipt:

a. Ensure both the agent laboratory and the MINICAMS laboratory have radios.

b. Ensure a STEL MINICAMS and IDLH MINCAMS are monitoring the agent laboratory.

c. Ensure an operable type of “gross detection equipment” is available for use in the agent laboratory.

d. Aquire mask from PC&E.

e. Set up glove box with:

(1) Hot trash container

(2) Paper towels

(3) M-8 paper

f. Prepare labels and receive issued roll of seals from the accountable officer for metal containers (side and top) and the vaults (mailing tubes may be identified with a COBRATF assigned traceable vial number).

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g. Prepare required accountability paperwork, if needed.

h. Ensure metal containers for storage cans are:

(1) Properly labeled with NFPA labels

(2) Contain a small amount of vermiculite in the bottom

(3) Taped around the lid of the can

i. Ensure agent laboratory personnel present are equipped with respiratory protection.

j. Obtain appropriate vault keys from A and B box (two-man rule).

k. Ensure appropriate tools required to open LSC are nearby.

l. Place the agent operation sign in the agent laboratory window.

NOTE 2: Receiver notification from safety control when ECBC tech escort personnel are entering the training building.

4. Unpacking/Re-Packing

a. Take custody of neat agent inside the agent laboratory; not at the guardhouse.

b. If needed, use dolly to move wooden crate and LSC inside the Neat Agent Laboratory.

c. Tech escort personnel bring containers of neat agent to the agent laboratory.

d. The Custodian receiving the agent checks the LSC serial number around the seal rings,verifies it with the DD Form 1911. Once the inventory is complete the Primary Custodian or Accountability Officer will review and sign paperwork to accept custody.

NOTE: If DOD agent identifying information is located on the DD1911, the custodian receiving the agent, will confirm the agent identifying information that is on the agent received is the same identifying information that is annotated on the DD1911 and other receiving documents. If there is a discepency refer to section P.4.d

e. All personnel present inside the agent laboratory must don NIOSH-certified APR, butyl gloves and LANX suit or equivilant, before removing the LSC from the wooden crate.

f. Remove LSC from the wooden crate and place by the MINICAMS sample point in front of the vault hood.

g. Visually examine the LSC for obvious signs of damage.

h. Note results on the receipt document.

i. Visually inspect the outside of the LSC for leakage:

(1) Check any liquid observed with M-8 paper

(2) If agent is found outside engineering controls, place LSC in vault hood and notify safety control portuesep Typewritten Text portuesep Typewritten Text portuesep

(3) Safety control will then assume responsibility for further notification

j. If no damage or leakage is found, notify MINICAMS laboratory personnel that the agent laboratory is ready to unpack the LSC.

k. When the IDLH MINICAMS monitoring the front of the vault hood starts a new cycle, the MINICAMS laboratory notifies the agent laboratory to start unpacking the LSC.

l. Notify safety control prior to unpacking neat chemical agent material from the

LSC.

m. Remove the sealing wire and loosen closure bolts in a diametrically opposite pattern (i.e. loosen first bolt, then second bolt directly across diameter of LSC top).

n. Remove the top and place on a cushioned surface to preclude scratching machined sealing surface.

o. Remove rubber spacer and fiberboard container that contain the ampoules.

p. Place the fiberboard container in the vault hood.

q. Repeat until LSC is empty.

r. Repacking empty LSC:

(1) Place rubber spacers back into the LSC.

(2) Carefully replace top on the LSC after ensuring the O-rings are still in the O-ring groove

(3) Install every other closure bolt back into the bolt holes and place remaining bolts inside container(s)

(4) Hand tighten bolts securely

(5) Place the LSC back into the wooden crate

(6) Move the crate out of the way

(7) If necessary, use dolly

s. Repeat steps k through r above for each wooden crate received.

t. Notify the MINICAMS laboratory that all agent is under engineering controls.

u. When the current STEL cycle is complete, the MINICAMS laboratory gives the agent laboratory the “all clear”.

v. Agent laboratory personnel then remove Niosh-certified APR, butyl gloves, and LANX suit.

5. Inventory of New Neat Agent

a. Agent laboratory personnel must wear exam gloves and lab coats, with NIOSH-certified APR nearby.

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b. Transfer fiberboard container from the vault hood into the glove box one agent at a time:

(1) Remove mailing tubes

(2) Remove ampoules from mailing tubes (one at a time)

(3) Let mailing tubes lay flat on the floor of the glove box

(4) Do not keep mailing tubes in an upright position to lessen the likelyhood of vial breakage

c. Pass metal containers and labels into the glove box.

d. Remove ampoules from mailing tubes leaving a small amount of vermiculite in the mailing tube (one mailing tube at a time).

e. If vials are leaking proceed and handle IAW section S.3.C.

f. Inspect one ampoule at a time:

(1) Gently remove the vermiculite particles

(2) Check with M-8 paper

(3) Inventory the lot number, vial number and amount

(4) Label the ampoule IAW Q.1.c(2)

(5) Place the ampoule back into the mailing tube and close

(6) Write the vial number on the cap

(7) Place mailing tube into the new container

g. Repeat steps d and e above for each mailing tube from the fiberboard container.

(1) Sealed containers that contain agent are to be used at a later date.

(2) Place emptied fiberboard container into the vault hood

h. Repeat steps b through f above for each container.

i. After filling a new container:

(1) Place label on the side of the container

(2) Place label on the top of the container

(3) Secure the lid to the container with tape

(4) Place the new container inside the appropriate vault

6. Upon completion of inventory:

a. Secure vaults (seal vault with tamper evedent seals, if needed).

b. Clean glove box with a bleach soaked towel, then rinse with water

c. Clean vault hood with water

d. Double bag empty fiberboard containers and any other trash portuesep Typewritten Text portuesep Typewritten Text portuesep

e. Label vaults

f. Change glove box gloves per section I.2.b of this procedure

g. Place hot trash and gloves into pass-thru chute

h. Notify safety control that hot operation is complete

7. The Accountable Officer ensures all accountability paperwork per section Q of this procedure is completed.

8. Special Requirements

a. The LSCs are designed for reuse and shall be returned to the Chemical Transfer Facility (CTF) to be reused for further shipments.

b. REMOVE ALL LABELS BEFORE SHIPPING LSC except “This end up” label on the plywood box.

c. The Accountable Officer must follow shipper’s receiving and handling instructions prior to and/or once the neat chemical agent material is received.

9. General information regarding shipment of neat agent

a. The mailing tubes (note: only one ampoule per mailing tube) are placed in a fiberboard container.

b. Generally 6 to 8 mailing tubes for 20 mL capacity ampoules and 5 mailing tubes for 40 mL capacity ampoules are allowed per fiberboard container.

c. Any void is filled with vermiculite.

d. The fiberboard container is placed in the LSC. The LSC can hold 3 fiberboard containers, separated by rubber spacer. Rubber spacers are used as filler.

e. The LSC is placed in a strong wooden box (wooden crate) for stability during shipment. This strong wooden box is also known as a “shipping container”.

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P. Accountability

1. Neat Agent:

a. A flow chart for neat agent request procedures and a flow chart for accountability procedures are included at the end of this procedure.

b. All transfers between primary custodians of different agencies are executed on DD Form 1911 to prove chain of custody. The losing primary custodian prepares DD Form 1911. DD Form 1911 and CQATFM-SR-1 and are used to document the receipt of a chemical agent from ECBC.

c. CHEMF 003 and CHEMF 004 are initiated upon receipt of chemical agent material. Complete the top portion of CHEMF 003 except leave the “Balance of Agent Brought Forward” blank for each vial in the lot.

(1) The amount of chemical agent material that is accounted for will be the amount of agent contained in pre-measured ampoules.

(2) A lot number is established by the chemicial agent laboratory when agent is received. The number is derived based on what type of age sequence in which it was received, identifies the particular lot of aent received. For example, the first batch of agent GB received on 20 Aug 99 (Julian date 99232) is assigned lot number 992321G. Accountability records are referenced according to agent lot number.

d. Agent Custodians conducting receipt of the agent and the Accountable Officer must sign CHEMF 004. The Accountable Officer verifies information listed on CHEMF 004 against DD1911 in the presence of an Inventory Officer.

2. Labeling Neat Agent Containers

a. Once all agent has been received and inventoried, all mailing tubes containing agent ampoules are placed inside a secondary container (slip-top metal can).

Once the lid is taped on the container, a tamper resistant security seal is placed on the can covering the end of the tape.

b. When storing secondary containers in the vault, if tamper resistant security seals are on all secondary containers within the vault, tamper resistant security seals may be placed on each side of that particular closed vault lid. Once the vault is sealed with two tamper resistant securtity seals, it is not necessary to re-open each vault during subsequent inventories until those seals have been removed.

It is only required to verify the control numbers on each of the security seals during each subsequent inventory until the seals have been removed.If seals have been damaged or deteriorating, contents will be inventoried and re-sealed.

c. The outer secondary container (e.g. slip-top metal can) is labeled using CHEMF 007 and contains the following:

(1) Type of agent

(2) Amount of agent contained (mL)

(3) Operating activity, building number and room number file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20003.0%20Custodial%20Stock%20Record%20Form.pdf file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20004.0%20Receipt%20of%20Neat%20Chemical%20Agent%20Reference%20Record.doc file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20003.0%20Custodial%20Stock%20Record%20Form.pdf file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20004.0%20Receipt%20of%20Neat%20Chemical%20Agent%20Reference%20Record.doc file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20004.0%20Receipt%20of%20Neat%20Chemical%20Agent%20Reference%20Record.doc portuesep Typewritten Text portuesep

UNCONTROLED

(4) Name and telephone number of custodian

(5) Date placed in storage

(6) Special instructions

Figure 1.

Sample Container Label

Figure 2.

Sample Vial Label

d. The primary containers (i.e., vials/ampoules) are labeled by ECBC; however, if it is necessary, each vial/ampoule is assigned an identifying number by the agent laboratory, which is entered onto CHEMF 004.

e. After each usage of agent, the label on the vial, secondary container and the corresponding vault are updated.

f. The individual pre-measured containers (e.g. syringes) used to transfer the agent to training aids are labeled.

EXAMPLE

TOXIC CHEMICAL

AGENT NAME OR CODE ______________________________

QUANTITY (METRIC) ____ __ ________________

OPERATIONAL ACTV/BLDG/ROOM _____________________

CUSTODIAN/TEL NO. _________________________________

DATE STORED _______

DANGER

VX, 990051-V

VIAL 15A

9.00 mL

9 M 00 file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20004.0%20Receipt%20of%20Neat%20Chemical%20Agent%20Reference%20Record.doc portuesep

g. Each transaction involving agent material is entered on CHEMF 003 for that lot number and vial number and authenticated by the Agent Custodian/Accountable Officer.

h. Each entry on CHEMF 003 is substantiated by a supporting document such as a chemical agent material receipt, shipping certificate, inventory certificate, etc.

i. When agent material of a particular lot is expended, CHEMF 003 is closed out.

3. Chemical Agent Material Preparation and issue:

a. CHEMF 005 is prepared each time agent materials change custody.

b. Enter the type of agent, lot number, number of primary containers (syringes) prepared, a description of the primary container (vial number), the total quantity of agent (in mL) used and the type of training or other appropriate comment in the blanks provided.

c. The issuing individuals print/type their name and sign in the space provided.

d. The receiving individuals sign the “received by” portion of the form.

e. The receiving individuals complete the destruction statement block when the agent is destroyed, decontaminated, or placed on training aids in the course of training.

f. Safety personnel complete the “Safety Persons” portion by signing in the space(s) provided.

g. All individuals are responsible for ensuring information on CHEMF 005 is correct prior to signing.

NOTE 1: When additional Agent Custodians are needed, their names will be added to

CHEMF 005.

h. Decontamination of agent in laboratory operations is documented on CHEMF 005.

The laboratory personnel issue to themselves and complete the destruction statement.

i. Keys to the locked containers (vaults) are secured in the A and B key boxes and are signed out by authorized personnel on CHEMF 010 as needed. Two trained responsible personnel who are CERP qualified are present at all operations involving access to chemical agent material.

NOTE 2: Agent laboratory personnel assigned as the Accountable Officer and Chemical Agent Custodian must be trained IAW this procedure. An appointment letter signed by the CDP Superintendent is required, and Agent Custodians must be certified to handle agent.

4. Inventories:

a. Inventories of chemical agent materials are conducted semi-annually and when agents are received, upon transfer to a new primary custodian, or as directed by the AO. Complete CHEMF 009 upon completion of inventory.

b. During the receipt of neat agent, once the agent has been inventoried and tamper resistant security seals have been applied, all control numbers are logged on file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20003.0%20Custodial%20Stock%20Record%20Form.pdf file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20003.0%20Custodial%20Stock%20Record%20Form.pdf file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20003.0%20Custodial%20Stock%20Record%20Form.pdf file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20005.0%20Chemical%20Agent%20Material%20Receipt.xls file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20005.0%20Chemical%20Agent%20Material%20Receipt.xls file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20005.0%20Chemical%20Agent%20Material%20Receipt.xls file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20005.0%20Chemical%20Agent%20Material%20Receipt.xls file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20010.0%20Key%20Control%20Register%20and%20Inventory.pdf file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20009.0%20Chemical%20Agent%20Material%20Inventory%20Certificate.docx portuesep receipt of neat agent form and verified each subsequent inventory until the seals have been removed.

c. The Accountable Officer completes CHEMF 009 and forwards appropriate receiving information to the ECBC Accountable Officer as soon as the documents are signed by the proper authority.

d. Discrepancies are reported to AMC and the ECBC Accountable Officer IAW an IAA. If the discrepancy cannot be resolved (per step c above) immediately, the Accountable Officer initiates required action based on a coordinated effort between the ECBC Accountable Officer and the CDP Accountable Officer.

e. Once inventory is complete (scheduled or unscheduled), custodians make an entry on CHEMF 003 in red ink, showing inventory completed on that date and balance of agent.

f. Upon receipt of the inventory results, the Accountable Officer reconciles the accountability records. An entry is made on CHEMF 003 and the inventory balance is entered.

g. Inventory adjustments may be made automatically IAW the Memo For Record, Subject: Maximum Allowable Amount of Chemical Agent Accountable without Causative Research. If discrepancies exceed this amount, the appropriate procedures are implemented.

h. Semi-annual reports are submitted to the DoD Accountable Officer per an IAA with DoD. Instructions on information needed are sent to the Accountable Officer prior to each semi-annual inventory..

i. The inventory reports and the monthly agent utilization reports are distributed according to the chart below.

Intentionally left blank file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20009.0%20Chemical%20Agent%20Material%20Inventory%20Certificate.docx file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20003.0%20Custodial%20Stock%20Record%20Form.pdf file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20003.0%20Custodial%20Stock%20Record%20Form.pdf portuesep Typewritten Text portuesep

Chart 1.

Accountability Reports Distribution

Report Name E-mail Copy Dissemination Hard Copy Storage

Inventory Report ECBC Accountable Officer HQ AMC Surety Divison

Accountable Officer keeps original documents in proper book keeping order

MAUR ECBC Accountable Officer Accountable Officer keeps original documents in proper book keeping order

Shipment Status Report

CASARM Quality Assurance Team ECBC Accountable Officer

Original documents are kept in proper book keeping order inside the agent laboratory

Q. Syringe Operations in the Glove Box

NOTE: As long as all procedures are performed, they do not have to be performed in the order which they are written.

1. Pre-Agent Operations

a. Prepare Syringes

(1) Obtain syringe barrels (Beckton Dickson BD syringes, if possible) and needles from supply technician

NOTE 1: Do not use syringes made of polypropylene or polyvinyl because VX and GB are incompatible with those polymers.

(2) Cut the needles leaving a blunt end on each needle, (a purchased blunt needle may be used also, as long as they are long enough to reach the end of the vial).

(3) Remove the metal insert from the needle and discard it in a sharps container.

(a) This can be done inside the glove box or outside the glove box as directed by the Chemist. Cut the needles for the syringes used to pour agent ¼” to ½” long. (If needles are uable to be shortened in the glove box during agent operations short ones will be cut at this time, as well, to be exchanged out for a long needle during the operation prior to being passed into the hot area.

(4) Replace cap each needle after cutting it.

(5) Place clear tape over label

(6) Colored electrical tape may also be used to distinguish agent or syringe types

b. Obtain one plastic beaker for hot trash/waste generated in the glove box. Ensure beaker is labeled to indicate contents.

c. Fill out form CHEMF 007 to prepare labels for secondary container(s) and vaults.

d. Prepare labels for vials.

file://amcd12f01/forms/CDP%20COBRATF%20FORMS/CHEM%20Forms/CHEMF%20007.0%20Danger%20Label.pdf portuesep

NOTE 2: See section Q of this procedure for description and content of labels.

e. Prepare Gloves:

(1) Ensure a set of glove box gloves (non-standard) covered with chemical protective gloves (standard) is available for glove change

(2) Check serviceability and expiration dates of all gloves before use

(3) Ensure another appropriately sized set of standard gloves is available for exchange in the vault hood

f. Prepare chemical agent container:

(1) The chemical agent container used to hold and transfer the syringes to the hot area is placed in the pass-thru chute by the Agent Custodians

(2) Ensure the box contains appropriate plastic container(s) for decon solution

(3) Place at least two paper towels in the chemical agent container

(4) Transfer the chemical agent container to the glove box

g. Prepare glove box

(1) Ensure the following items are in the glove box:

(a) Syringes in the tray

(b) Hot trash beaker

(c) Paper towels

(d) Septums in glass ashtray (enough for the total number of syringes)

(e) Tags for hot trash and used gloves

(f) Plastic cup

(g) Bleach squirt bottle and water squirt bottle

(h) Container to collect sharp needle ends; with bleach for VX and 10% NaOH (caustic soda) for GB. These are placed in the needle cutter prior to the use of agent

(i) Labels for vials and secondary containers

(j) Vial rack/holder

(k) Vial rack tray

(l) Large tweezers

(m) Decon solution

2. Agent Operations

NOTE 3: Lab coat and nitrile gloves must be worn by all agent laboratory personnel participating in the operation.

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a. Place the agent operations sign in the agent laboratory door window.

b. Verify with safety control the number of syringes, quantity of agent needed and names of the Agent Custodians.

c. Verify with safety control that the IDLH MINICAMS is monitoring the agent laboratory.

d. Obtain keys to vault.

e. Agent operations may start once the agent laboratory has been notified by safety control that all support personnel are in place and the agent laboratory has three (3) personnel in place.

f. Access vaults using the two-person concept described in the Chemical Hygiene Plan.

g. Use both hands to transfer the agent container(s) for all agents of interest from vaults to glove box pass thru chute.

h. Move to the glove box.

NOTE 4: If glove of one hand becomes loose while preparing agent syringes, make sure other hand is free to adjust glove. For example, if an object (syringe) is in one hand, put object down on pan/floor, then adjust loose glove. Do not adjust the glove while holding the syringe (or any other object) in one hand.

(1) Use only one type of agent at a time

(2) Remove container(s) for the first agent for use from glove box pass thru chute

(3) Remove tape from lid of container

(4) Remove vial from container

(5) All agent laboratory personnel must ensure proper vial is removed.

i. Fill syringes in the glove box

(1) Place vial in vial rack/holder

(2) Unscrew top of VX or GB vial and place lid in vial rack tray

(3) Check gloves for any contamination and decon with bleach, if necessary

(4) Pick up appropriate syringe with long needle and remove needle cap. Place cap in hot trash waste container.

(5) Fill syringe to the mark as indicated on label

(6) Invert the syringe and draw the remaining agent from the long needle.

(7) Remove the long needle and place it in the cup, keeping syringe inverted.

(8) Check the glove of the hand that removed the needle for agent.

(9) Slowly push the air out of the inverted syringe.

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(10) Place the short needle on the syringe, ensuring that the needle is secured to the syringe.

(11) Check to see that the barrel of the needle is not pinched.

(12) Cap needle with septum. Be careful not to press needle through septum.

(13) Place filled and capped syringes in syringe tray.

(14) Discard the long needle in the appropriate sharps container when VX or GB is complete, do not cross contaminate.

(15) Repeat steps (1) – (14) above for each syringe.

(16) Close, shake, and burp the sharps/caustic container after each agent needle or ampoule top is added.

j. Return agent to glove box pass-thru chute.

k. Repeat steps h – j above if other agent is needed.

l. Use both hands to transfer each container to the appropriate vault(s).

m. Transfer the chemical agent container into the glove box if it has not already been done.

n. Carefully transfer all the syringes containing agent from the syringe tray to the chemical agent container while rechecking accountability.

o. Inform safety control that the chemical agent container is ready.

p. Secure locks in vault hood.

q. When Agent Custodians are ready for the chemical agent container:

(1) Unlatch the door on the vault hood side of the glove box pass-thru chute.

(2) Transfer the chemical agent container from the glove box to the glove box pass-thru chute.

(3) Use both hands to remove the chemical agent container from the glove box pass-thru chute and place box on floor of vault hood while latching door to glove box pass-thru chute, ensuring that one hand is on the chemical agent container while it is resting on the vault hood floor.

(4) While keeping one hand on the chemical agent container, open the toxic agent training area pass-thru chute door.

(5) Use both hands to transfer the chemical agent container to the toxic agent training area pass-thru chute and close the door.

NOTE: All communication between laboratory personnel and Agent Custodians are conducted via radio.

(6) Notify Safety Control and Agent Custodians that the agent container is ready for training.

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(7) The Agent Custodians notify laboratory personnel when they are ready to receive the agent container.

(8) Agent Custodians wait for laboratory personnel to tell them that the agent container is in the pass-thru chute before taking possession.

(9) An Agent Custodian removes the agent container from the pass-thru chute.

(10) The Agent Custodians inform laboratory personnel that they have possession of the agent container and report the inventory of the container. Once inventory of the container has been reported, laboratory agent custodians will verify proper amounts.

(11) Training Control logs contents of the agent container into WebEOC.

(12) In the event that too much time elapses prior to Agent Custodians taking possession of the container of agent, the Laboratory Agent Custodians will retrieve the container and return it to the glove box. Laboratory Agent Custodians will determine timeframe on a case by case basis.

3. Semi-Annual Agent Inventory

a. The Accountable Officer ensures an Inventory Officer is appointed who serves as the Witnessing Official during neat agent inventory.

(1) The Inventory Officer shall be knowledgeable and not rated by either the Accountable Officer or Agent Custodian.

(2) The Inventory Officer is briefed by the Agent Custodian(s) and the Accountable Officer on the conduct of the inventory prior to beginning the inventory.

(3) A 100% physical inventory is conducted and witnessed by the Inventory Officer for all agents.

(a) To minimize agent loss, the Agent Custodian conducts the physical inventory “to eyeballing” rather than drawing into a syringe.

(b) If necessary, the Agent Custodian conducts all volumetric determinations (by drawing into a syringe) under the observation of the Inventory Officer.

(c) Vials/syringes filled with water may also be used by the Agent Custodian to show a comparable volumetric determination to the actual amount contained in the agent vial.

b. Inventory Procedures

NOTE 5: Lab coat and nitrile gloves must be worn by all agent laboratory personnel participating in the inventory.

NOTE 6: Agent operations start once the agent laboratory is notified by safety control that all support personnel are in place, the agent laboratory has three (3) personnel in place, and the Inventory Officer is present.

(1) Laboratory Personnel:

(a) Place the agent operations sign in the agent laboratory door window.

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(b) Verify with safety control that MINICAMS is monitoring the agent laboratory for IDLH.

(c) Obtain the keys to the vault.

(d) Access vaults using two-person concept described in the COBRATF Chemical Hygiene Plan.

(e) Use both hands to transfer the agent container(s) for all agents of interest from vaults to glove box pass-thru chute.

(f) If the secondary container still has a security seal on the container, the seal may be inventoried at the vault and placed back in the vault once all containers with seals stored in that vault have been inventoried.

(g) If necessary, the custodian may move all containers to the glove box to inventory sealed and unsealed containers.

(h) Move to the glove box and remove the first container for inventory.

(i) Remove tape from lid (if it does not have a security seal covering the tape of secondary container

(ii) Remove vial(s)/mailing tube from the secondary container

(iii) Ensure all vials are removed from secondary container

(iv) Remove each vial from mailing tube, if applicable

(v) Place vial(s)/mailing tubes back into secondary container

(vi) Place labels on secondary container(s)

(vii) Return tape to lid of secondary container

(viii) Tranfer secondary containers into glove box pass thru chute

(ix) Use both hands to transfer agent container(s) from glove box pass thru chute to vaults

(x) Continue this process for each vault

(2) Inventory Officer:

(a) Observe and verify, to include contents of label, all agent removed from each vault.

1. Observe and verify each vial removed from canister and mailing tubes.

2. Observe and verify each container being emptied.

3. Record all observations pertaining to information concerning amounts of agent

(3) Cracked vials:

(a) If a cracked vial is found during the inventory process, check it with M8 paper.

file://amcd12f01/forms/UNCONTROLLED%20SOPs,%20QMPs,%20Manuals%20and%20Plans/CDP%20Safety%20Plan%20Annexes/Annex%20L%20Chemical%20Hygine%20Plan.pdf portuesep

(b) If the vial is leaking, measure contents with a syringe and transfer to another vial keeping the initial identification and record amount.

(c) If the vial is not leaking, place it in a sealed bag and mark to be checked for leakage at the next inventory.

c. Once semi-annual inventory is complete, verify all paperwork IAW section D of this procedure.

4. Post Agent Operations

a. Wipe the inside of the glove box with a towel soaked with bleach; rinse with a water soaked towel ensuring to rinse away bleach residue.

b. Remove container with decon solution and cut needles from the needle cutter;

recap and place in hot trash double bag.

c. Double bag all trash/waste, to include butyl rubber gloves covering glove box gloves.

d. Replace standard gloves with another set of standard gloves.

e. Any gloves not contaminated with agent can be placed in a separate bag and labeled to go to appropriate bin.

f. Change glove box gloves IAW section A.6.f of this procedure.

g. Tag and…