This Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training (CFDA 93.859) program, provides $658,165 to Triad National Security, LLC to conduct advanced research on the effects of ions on mRNA elements, ribosomes, and antibiotic-RNA interactions. The research aims to gain a better understanding of the fundamental physics underlying ribosome operation and mRNA regulation, with a focus on the bacterial ribosome and...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) provides $678,000.00 to Rockefeller University to conduct research on the mechanisms of eukaryotic ribosome assembly. The long-term goal is to gain a detailed, atomic-level understanding of the molecular snapshots and transitions during the multi-stage assembly process, which is critical for protein synthesis in all cells. The research...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training (CFDA 93.859) program, provides $316,968.00 to Emory University to study the regulation, inhibition, and quality control mechanisms of ribosomes. The key research activities focus on three main themes: 1) understanding translation fidelity events, including miscoding, quality control, and mRNA frameshifting; 2) investigating ribosome rescue when...
This $1,444,500 Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training Program (CFDA 93.859) aims to develop new cryogenic electron microscopy (cryo-EM) methods to visualize the structural heterogeneity of ribosomes at the single-molecule level in cells. The award, effective September 2, 2024 through August 31, 2027, will enable the principal investigator to pursue strategies to extend a novel technique called 2D template...
This $543,105 federal Project Grant awarded by the National Science Foundation (NSF) under its Engineering program (CFDA 47.041) aims to investigate an approach for creating an optimized ribosome system to efficiently produce designer proteins. The key objective is to construct a ribosome that is hardwired to synthesize a specific protein by incorporating the genetic message directly into the ribosome structure. This "iRibo-T" system is expected to isolate the ribosome from normal...
This federal Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training program (CFDA 93.859), is providing $316,825 to Massmatrix Inc. to advance native mass spectrometry (MS) analysis capabilities. The key objectives are to: 1) Implement enhanced deconvolution techniques to reduce data artifacts and optimize processing speed without sacrificing accuracy; 2) Automate spectrum reconstruction for efficient handling of...
This $390,000 federal Project Grant award from the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training Program (CFDA 93.859) will support the development of innovative proteomic technologies to study transcription factor-coregulator interactions. The award aims to pioneer DNA barcode-based methods that can enhance the specificity and reproducibility of existing techniques like mass spectrometry-based RIME for characterizing epigenetic...
This federal Project Grant award from the National Institute of General Medical Sciences (NIGMS), under the Biomedical Research and Research Training program (CFDA 93.859), provides $939,086 to Brigham Young University to develop advanced mass spectrometry-based techniques for profiling the proteome, lipidome, and post-translational modifications in single cells. The goal is to enable an unprecedented view of cellular regulation from genotype to phenotype at the single-cell level, which could...
This federal Project Grant award in the amount of $271,495.00, provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), supports the development and evaluation of a time-of-flight (TOF) mass spectrometer system with matrix-assisted laser desorption/ionization (MALDI) for fast analysis of antibodies and related macromolecular immune complexes. The goal is to utilize a novel high-temperature superconducting...
This federal Project Grant award of $419,375 was provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859). The award supports research to define the functional dynamics of multi-domain redox enzymes, such as nitric oxide synthase (NOS), which are crucial for diverse signaling processes and disease states. The research aims to provide unprecedented understanding of how regulatory interactions and modifications...