This Project Grant award, funded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), aims to advance the understanding of single-strand break (SSB) repair mechanisms within chromatin. The $1,207,500 award to Case Western Reserve University will support a 5-year research project to investigate how three key DNA repair proteins - PARP1, LIG3, and PNKP - recognize and repair SSBs in the context of nucleosomes. The research will employ DNA libraries, next-generation sequencing, biophysical tools, functional assays, and structural analysis techniques to map SSB accessibility, characterize protein-nucleosome interactions, and elucidate the interplay between post-translational histone modifications and SSB repair. The long-term goal is to provide a fundamental understanding of base excision repair mechanisms within nucleosomal contexts, which could inform the development of drugs and therapeutics for various disease states.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $402.5k | 6/6/25 | ||
| Not listed | $402.5k | 7/4/24 | ||
| Not listed | $402.5k | 7/4/24 |