This Project Grant award from the National Institutes of Health (NIH) under the Research Infrastructure Programs (CFDA 93.351) provides $240,039 to Baylor College of Medicine to develop a novel mouse model for detecting genome editing events. The project aims to create a more sensitive reporter system using CRISPR/Cas9 technology, where editing of a specific genetic domain triggers the expression of a fluorescent marker protein. This would allow for improved monitoring of genome editing events...
The Massachusetts General Hospital will receive $3,024,000 through a three-year Project Grant from the National Institutes of Health Trans-NIH Research Support program to develop scalable methods for engineering and characterizing novel CRISPR-associated protein variants. The goal is to address limitations of current CRISPR-Cas enzymes by optimizing machine learning-guided directed evolution to create a catalog of customizable DNA editors with improved properties like expanded target...
This Project Grant award from the National Institutes of Health (NIH) under the Research Infrastructure Programs (CFDA 93.351) provides $844,954 to New York University School of Medicine to develop "Genomically Rewritten and Tailored Humanized Mouse Models for Various Organ Disorders". The award leverages advanced DNA synthesis and genome editing technologies to create mouse models that accurately recapitulate human disease phenotypes. These "GREAT-GEMMS" (Genomically...
This Project Grant award from the National Institute of General Medical Sciences (NIGMS) Biomedical Research and Research Training program (CFDA 93.859) supports research to improve the efficiency of CRISPR gene editing in human pluripotent stem cells. The goal is to develop a novel carbon nanotube array technology that can efficiently transfer nucleic acids and proteins into these cells to enhance CRISPR-based gene editing and homology-directed recombination. The $275,045 award to Advanced Gene...
The National Institutes of Health (NIH) awarded a $265,500 Project Grant under the Research Infrastructure Programs (CFDA 93.351) to the Jackson Laboratory in Bar Harbor, Maine. The grant, titled "Rapid Development of Mouse Resource Strains Through the Application of Prime Editors," aims to develop advanced mouse models and research tools through the use of prime editing technology. The key objectives of the project are to exploit prime editing to efficiently introduce functional DNA...
This $3,834,112 Project Grant awarded by the National Human Genome Research Institute (NHGRI), under the Human Genome Research program (CFDA 93.172), aims to develop and refine computational tools to advance the safety and efficacy of genome editing technologies. The key products and services to be delivered include: Enhancing the CRISPRME tool to provide more comprehensive prediction of off-target effects of genome editing across diverse human genomes, and developing a new tool called...
This Cooperative Agreement award from the National Institutes of Health (NIH) Office of the Director, under the Trans-NIH Research Support program (CFDA 93.310), provides $856,513 to The Children's Hospital Corporation (doing business as Boston Children's Hospital) to develop an integrated pipeline for assessing variant-associated off-target risks in genome editing therapeutics. The project aims to optimize and integrate the CRISPRME computational tool and ABSOLVE-SEQ experimental method to...
This Project Grant award, provided by the National Institute of Child Health and Human Development (NICHD) under CFDA 93.865 Child Health and Human Development Extramural Research, supports the development of high-throughput methodologies to rapidly measure and optimize in vivo adeno-associated virus (AAV)-mediated homology directed repair (HDR) gene editing efficiency in mice. The $425,348 award to Indiana University Indianapolis, a research-intensive state university, aims to create novel...
This $143,800 Project Grant award from the National Institutes of Health (NIH) under the Research Infrastructure Programs (CFDA 93.351) supports the development of mouse models for targeted manipulation of DNA methylation at specific genomic loci. The goal is to create simple, robust tools for epigenetic gene targeting that can advance functional studies of DNA methylation and its role in human health and disease. Specifically, the awardee, Baylor College of Medicine, will develop two mouse...
This Project Grant award from the National Institutes of Health's (NIH) Research Infrastructure Programs (CFDA 93.351) provides $775,888 to the La Jolla Institute for Immunology to develop and validate a novel CRISPR-ready, HLA transgenic mouse platform. The platform will enable researchers to rapidly generate mice expressing specific HLA class I and II alleles relevant to a broad range of diseases, including infection, cancer, autoimmunity, and transplant rejection. This versatile platform will...