This $117,074 five-year federal Project Grant award was provided by the National Science Foundation's (NSF) Mathematical and Physical Sciences program (CFDA 47.049) to Lehigh University to support research into biomaterial-guided CRISPR gene editing delivery systems. The project aims to systematically explore how the physicochemical properties of biomaterials and nanoparticles, as well as cell-material interactions, can be leveraged to enhance the spatiotemporal control, efficacy, and safety...
This Project Grant from the National Science Foundation's Technology, Innovation, and Partnerships program totaling $100,000 will support research to develop next-generation clustered regularly interspaced short palindromic repeat (CRISPR) systems for curing inherited diseases. Specifically, the awardee, the University of California, Berkeley, will work to circumvent in vivo delivery challenges with CRISPR-Cas9 by developing miniature RNA-guided genome editors smaller than Cas9 that can be...
This federal Project Grant award from the National Human Genome Research Institute (CFDA 93.172 - Human Genome Research) provides $3,834,112 to develop and refine computational tools to advance the safety and efficacy of genome editing. The key products and services to be delivered include: Enhancing the CRISPRME tool to better predict off-target effects of genome editing across diverse human genomes. A new tool, CRISPRUS, will also be developed to leverage variation graphs, complete genomes,...
The National Science Foundation (NSF) awarded a $274,438 SBIR Phase I grant to Script Biosciences Inc. under the NSF Technology, Innovation, and Partnerships (CFDA 47.084) program. The project, titled "CASPLUS: A Safer, More Efficient, and Targeted Method of Deploying CRISPR In Vivo," aims to develop a platform technology that significantly reduces the potential inaccuracy of current gene editing techniques. The innovation targets the desired gene, increasing the safety and efficacy of...
The National Institute of General Medical Sciences (NIGMS) awarded a 3-year, $360,100 Project Grant under CFDA 93.859 (Biomedical Research and Research Training) to The Regents of the University of California, Berkeley (UC Berkeley) to develop precision base editing enzymes capable of correcting disease-causing point mutations in the genomes of hematopoietic stem and progenitor cells (HSPCs). The project aims to expand the scope of diseases amenable to CRISPR-mediated genetic correction by...
This federal Project Grant award of $200,000, made by the National Science Foundation (NSF) under the Engineering program (CFDA 47.041), aims to integrate non-viral CRISPR gene activation and deep learning to understand the impact of cell manipulation and gene activation on stem cell differentiation. The key objectives are to: (1) decouple the effects of cell transfection and CRISPR-mediated activation (CRISPRa) on mesenchymal stromal cell (MSC) therapeutic capacity, and (2) bridge the gap...
The Massachusetts General Hospital will receive $3,024,000 through a three-year Project Grant from the National Institutes of Health Trans-NIH Research Support program to develop scalable methods for engineering and characterizing novel CRISPR-associated protein variants. The goal is to address limitations of current CRISPR-Cas enzymes by optimizing machine learning-guided directed evolution to create a catalog of customizable DNA editors with improved properties like expanded target...
This $325,000 project grant awarded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) will support the development and testing of a new class of programmable, non-viral gene therapy carriers and cargo known as virus-inspired DNA origami (VIDO) vectors and repair templates. The project aims to demonstrate that VIDO vectors can effectively encapsulate and co-deliver CRISPR-Cas9 gene editing agents and repair...
This $398,774 Project Grant award, funded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training Program (CFDA 93.859), aims to develop a strategy for efficient delivery of single-guide RNA (sgRNA) through feeding in C. elegans to enable microinjection-free CRISPR/Cas applications. The primary objectives are to: 1) study RNA uptake in different C. elegans tissues using an RNA sensor strain, 2) design optimized RNA structures for enhanced...
The U.S. Department of Health and Human Services' National Institute of General Medical Sciences (NIGMS) awarded a $1,638,033 project grant under CFDA 93.859 (Biomedical Research and Research Training) to the Scripps Research Institute in La Jolla, California. The grant supports the development of a CRISPR-free mammalian recombineering system that enables large-scale DNA insertions, deletions, and substitutions in mammalian cells without the need for cytotoxic double-strand breaks. The project...