This Project Grant award of $234,000.00 from the National Institute on Drug Abuse (NIDA), under the Drug Use and Addiction Research Programs (CFDA 93.279), will support research conducted by Oregon Health & Science University (OHSU) to investigate rapid protein translation in nucleus accumbens neurons in response to a cocaine-associated cue. The key objectives of this research project are to: 1) Use translating ribosome affinity purification coupled with RNA sequencing (TRAP-seq) to identify mRNAs that are actively translating in the two main subtypes of nucleus accumbens medium spiny neurons (D1- and D2-expressing) during cue-induced cocaine seeking in an animal model, and 2) Validate the expression of approximately 10 of these translationally-regulated mRNAs at both the mRNA and protein levels. This innovative approach combining transgenic rodent models, TRAP-seq, and a translationally-relevant cocaine self-administration paradigm aims to elucidate the molecular mechanisms underlying cue-induced cocaine craving. The project will be conducted over a 2-year period from April 2025 to March 2027.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $234.0k | 4/14/25 |