Project Grant R03DE035162

Award Date 7/15/25
Completion Date 7/14/27
Dollars Obligated $315K
Federal Grant Program
93.121
Assistance Type
Project Grant
Place of Performance
Delaware, USA
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This Project Grant award from the National Institute of Dental and Craniofacial Research (NIDCR), under the Oral Diseases and Disorders Research (CFDA 93.121) program, is focused on studying the role of the SIX1 transcription factor in cell fate decisions within the mouse mandibular arch. The total funding amount is $315,289, with a project period from July 15, 2025 to July 14, 2027.

The key objectives of this award are to: 1) Identify changes in cell populations within the mouse mandibular arch of embryos carrying the P.R110W variant of SIX1 and compare them to wild-type and SIX1 knockout embryos using single-cell RNA sequencing and spatial transcriptomics; and 2) Determine the effects of SIX1 variants on osteoblast and chondroblast differentiation by using cultured neural crest cells and guided differentiation towards cartilage or bone, followed by quantitative cytochemistry and qPCR. This research aims to provide novel insights into SIX1 function during neural crest cell fate determination within the mandibular arch and characterize a new mouse model that can be used to study SIX1-related birth defects.

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