The National Eye Institute (NEI) awarded a $388,750 Project Grant under the Vision Research program (CFDA 93.867) to The Washington University in St. Louis. The five-year grant, effective August 1, 2024, will fund research to investigate the role of the histone variant H3.3 in retinal cell fate specification and differentiation. The research aims to complete a full phenotypic characterization of retinal development upon loss of H3.3 from retinal progenitors and post-mitotic neurons, as well as...
This $1,789,542 Project Grant award from the National Eye Institute (NEI), under the CFDA 93.867 Vision Research program, supports research to characterize the role of modular super-enhancers in regulating the expression of key transcription factor genes during retinal development in mice and humans. The awardee, St. Jude Children's Research Hospital, will leverage their Integrated Retinal Nucleome Database (IRNDB v.2) to identify developmental stage- and cell type-specific...
This Project Grant award from the National Eye Institute (CFDA 93.867 Vision Research) provides $146,922 to The Johns Hopkins University to conduct research on understanding the 3D chromatin architecture and gene regulation that control cell fate specification during mouse retinal development. The key products and services to be delivered include: Developing a single-cell Hi-C (schi-C) protocol adapted for droplet microfluidics to capture 3D chromatin interactions in mouse retinal cells at key...
This Project Grant award from the National Eye Institute (NEI), under the Vision Research federal grant program (CFDA 93.867), will provide $1,241,223 to the University of Pittsburgh to investigate the genetic and epigenetic mechanisms underlying retinal development and congenital vision disorders. The research aims to: Dissect the molecular, cellular, and genomic effects of genetic mutations on retinal development using mouse models. Elucidate how transcription factors wire the chromatin...
This is a Project Grant award from the National Eye Institute (NEI), under the Vision Research federal grant program (CFDA 93.867), in the amount of $249,000.00. The grant will fund research to investigate the molecular mechanisms that regulate the expression of phototransduction genes in mammalian rod photoreceptors. The research aims to elucidate how transcriptional elongation and mRNA processing are coupled to enhance the efficiency and accuracy of mRNA maturation, and how defects in these...
The National Eye Institute (NEI) awarded a $147,486 Project Grant (CFDA 93.867 - Vision Research) to The Johns Hopkins University to explore the development and connectivity of starburst amacrine cells in human retinal organoids. The project aims to establish timelines for the specification and subtype generation of these specialized interneurons, as well as characterize their synaptic connections within direction-selective visual circuits. Key objectives include using retinal organoid models to...
This Project Grant award from the National Eye Institute (NEI) under the Vision Research federal grant program (CFDA 93.867) is funding research to develop an innovative gene therapy approach using self-amplifying and self-splicing RNA technologies. The $346,500 award, spanning September 2024 to September 2026, aims to evaluate the longevity, immunogenicity, and potential in vivo side effects of this novel mRNA-based therapy for treating inherited retinal dystrophies. The award recipient, The...
This federal Project Grant award of $125,916 from the National Eye Institute (NEI) under the Vision Research program (CFDA 93.867) supports a research project entitled "Transcriptional and Developmental Basis of the Human X-Linked Homeotic Hotspot." The project aims to investigate the underlying molecular mechanisms driving a rare case of bilateral anophthalmia (missing eye globes) and XX sex reversal syndrome in a child. Key objectives include characterizing the genetic landscape of...
This Project Grant award from the National Eye Institute (NEI), CFDA Program 93.867 "Vision Research", supports research into retinal circuit integration and degeneration in neurofibromatosis type-1 (NF1). The $357,056 award to The Leland Stanford Junior University will fund a 2-year study to evaluate the functional impact of retinal ganglion cell (RGC) loss during NF1 progression, and examine if transplanted RGCs can form functional connections within the retina. The research aims...
This $214,227 Project Grant award from the National Eye Institute (NEI), under the Vision Research program (CFDA 93.867), supports a research project at Miami University to decode the transcriptional regulation of the FOXE3 gene during lens development. The project aims to identify and functionally test the cis-regulatory elements and transcription factors that control FOXE3 expression in the chick and mouse lens. Key approaches include employing recombinant retroviral vectors to test regulatory...