This $543,105 federal Project Grant awarded by the National Science Foundation (NSF) under its Engineering program (CFDA 47.041) aims to investigate an approach for creating an optimized ribosome system to efficiently produce designer proteins. The key objective is to construct a ribosome that is hardwired to synthesize a specific protein by incorporating the genetic message directly into the ribosome structure. This "iRibo-T" system is expected to isolate the ribosome from normal...
The National Science Foundation (NSF) Division of Molecular and Cellular Biosciences awarded a $750,000 Project Grant titled "TRANSITIONS: EVOLVING OUR UNDERSTANDING OF DYNAMIC RNA FOLDING AND FUNCTION" to Northwestern University under the Biological Sciences grant program (CFDA 47.074). The objective of this 3-year award is to uncover fundamental principles linking RNA sequence to dynamic RNA folding processes and function in biological systems. The research aims to enhance the...
The National Science Foundation (NSF) awarded a $1,199,997 Project Grant under its Biological Sciences program (CFDA 47.074) to the University of Colorado-Denver. The grant will fund research to improve understanding of the mechanisms underlying messenger RNA (mRNA) localization and translation, and develop machine learning models to better predict protein production from mRNA. The project will apply recent advancements in RNA sequencing technology to study how factors like tRNA abundance and...
This $299,779 National Science Foundation project grant under the Biological Sciences program (CFDA 47.074) funds research at Louisiana State University from August 2022 to July 2024 to design an RNA-based dual regulator for repetitive gene expression regulation. The research integrates biological experiments and computational studies to train scientists and engineers in interdisciplinary research. It also aims to cultivate a multidisciplinary perspective for problem-solving. Specifically, the...
The National Science Foundation awarded a $900,000 Project Grant to Texas A&M Agrilife Research under the Biological Sciences federal grant program (CFDA 47.074) from March 1, 2022 to February 28, 2025. The funding will support research focusing on the role of Coat Protein Complex I in transporting enzymes involved in regulatory functions like mRNA degradation and protein synthesis inhibition within plant cells. The grantee aims to elucidate how COPI contributes to miRNA-mediated RNA...
This National Science Foundation (NSF) Biological Sciences program grant to Yale University, totaling $940,000 over 3 years beginning March 1, 2024, aims to systematically identify features of human and viral messenger RNAs (mRNAs) that regulate protein production. The project will employ a massively parallel reporter assay to quantify the impact of RNA modifications, structured RNA elements, and cell-specific factors on mRNA translation. Insights gained will provide a comprehensive...
This National Science Foundation (NSF) Project Grant, awarded under the Biological Sciences program (CFDA 47.074), provides $750,000 to Stanford University to develop new technologies for studying RNA, the essential molecules that carry genetic information and play crucial roles in cell functions. The key products and services to be delivered under this 3-year award include: Creating non-destructive methods to directly study RNA localization and interactions within living cells, achieving...
The National Institute of General Medical Sciences (NIGMS) awarded a $965,356 Project Grant (CFDA 93.859) to Northeastern University to elucidate the mechanisms that enable translation in bacterial and eukaryotic ribosomes. The 5-year project, which began on May 1, 2024, will apply state-of-the-art molecular simulation methods to study how ribosomes accurately and efficiently produce proteins in cells. The research aims to advance the understanding of ribosome dynamics, which can inform the...
This $300,000 Project Grant from the National Science Foundation Division of Molecular and Cellular Biosciences will support research into the structural biology of RNA and RNA-protein interactions through May 2024. Funded under the Biological Sciences program (CFDA 47.074), this award to Vanderbilt University will utilize joint cryo neutron/x-ray crystallography techniques to better understand the importance of the 2'-hydroxyl group in RNA's expanded three-dimensional structure compared to DNA....
This $1,220,400 Project Grant awarded by the National Science Foundation (NSF) Division of Molecular and Cellular Biosciences supports a collaborative research project between investigators in the U.S. and U.K. to better understand the regulatory mechanisms controlling mammalian gene expression. The project, funded through NSF's Biological Sciences program (CFDA 47.074), aims to define the roles of different DNA regulatory elements and how they work together to precisely control gene expression....
The National Science Foundation (NSF) Division of Integrative Organismal Systems awarded a $299,983 Project Grant to North Carolina State University (NCSU) under the agency's Biological Sciences program (CFDA 47.074). The objective of this 2-year grant is to develop a new biotechnology called RIBOPI that will enable efficient, scalable, and cost-effective quantification of gene-specific translation regulation. RIBOPI aims to make translation regulation analysis as accessible as RNA sequencing has been for studying gene transcription. The proposed experimental pipeline involves testing novel combinations of in vivo and in vitro molecular biology procedures to efficiently and specifically map the first or last ribosome on each transcript, along with computational methods to compare the ribosome position distributions across experimental conditions. If successful, this technology could enable the discovery of gene regulation mechanisms underlying agriculturally important traits by reducing the cost, complexity, and sample requirements of current translation measurement approaches.