This three-year National Science Foundation project grant of $799,491 will fund research into the role of horizontal gene transfer in the evolution of antibiotic resistance among bacterial communities. The University of Washington will receive the funding to conduct three related research aims. First, the researchers will propagate communities of Escherichia coli, Salmonella enterica, and Klebsiella pneumoniae bacteria and monitor the evolution of a plasmid-borne gene conferring beta-lactam antibiotic resistance. By manipulating horizontal gene transfer between species, they will determine its role in the development of drug resistance. Second, genetically engineered bacteria will be used to identify mutations favored during horizontal transfer. Computational modeling will then predict whether such transfer accelerates or hinders resistance evolution. Third, a more realistic computational model incorporating complex environments, migration, competition will be tested against bacterial communities with horizontal transfer experimentally enabled or disabled through plasmid engineering. Overall, this award under the NSF's Biological Sciences program should advance understanding of horizontal genetic inheritance's influence on bacterial evolution and adaptation in changing conditions.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $799.5k | 1/28/22 |