Project Grant 20226701136543
- Berkeley Fermentation Science Inc., doing business as Berkeley Brewing Science, was awarded a $100,000 Project Grant from the United States Department of Agriculture National Institute of Food and Agriculture under the Small Business Innovation Research (SBIR) Program/Small Business Technology Transfer (STTR) Program. The grant funds research to engineer Saccharomyces cerevisiae yeast for increased biotransformation and production of tropical flavors from agricultural products. The SBIR/STTR...
- The Oregon State University will conduct research on the impact of Pediococcus species on wine chemical and sensory properties under a $123,599 project grant from the US Department of Agriculture Agricultural Research Service. The research will involve isolating and identifying Pediococcus bacteria from Oregon and Washington wines, screening isolates for sensory effects on standard wines, characterizing sensory impacts on red and white wines, and investigating factors affecting biogenic amine...
- This $350,000 project grant awarded by the National Science Foundation (NSF) Engineering program (CFDA 47.041) aims to develop a yeast strain with a robust protein secretion system that is tolerant to extreme pH and temperature levels. The primary objectives are to engineer the secretory pathway of the yeast Kluyveromyces marxianus using gene editing tools such as CRISPR, identify critical genes influencing protein production and secretion, and develop effective CRISPR libraries and selection...
- The National Science Foundation awarded a $422,834 Project Grant to the University of California Irvine under the Engineering federal grant program (CFDA 47.041) for research titled "Collaborative Research: Data-Driven Engineering of the Thermotolerant Yeast Kluyveromyces Marxianus." The three-year project period runs from October 1, 2022 to September 30, 2025. The award will support applying machine learning and deep learning techniques to metabolic engineering of yeast for...
- The National Science Foundation (NSF) awarded a $955,876 Project Grant to Northwestern University to investigate the role of prion-mediated epigenetic regulation in the budding yeast, Saccharomyces cerevisiae. The project, funded through NSF's Biological Sciences program (CFDA 47.074), aims to examine how two different yeast prions, [SWI+] and [MOT3+], interact and impact global transcription and protein homeostasis. Using advanced multi-omics techniques, the research program will identify...
- The National Institute of General Medical Sciences (NIGMS) awarded a $415,520 Project Grant under the Biomedical Research and Research Training program (CFDA 93.859) to the College of William & Mary. This 3-year grant supports research to investigate the genetic basis of virulence traits in opportunistic fungal pathogens and how these traits may arise through "accidental virulence" or "coincidental selection" in the environment. The research will screen over 1,000...
- This two-year, $200,000 Project Grant from the National Science Foundation's Division of Chemical, Bioengineering, Environmental, and Transport Systems will fund research at the Long Beach Research Foundation to develop multi-activity proteins for improved carbohydrate deconstruction. Specifically, the Foundation will produce and test 85 engineered multi-activity proteins containing two catalytic domains each. These proteins aim to increase biomass conversion for alternative fuels and...
- This Project Grant award from the National Science Foundation (NSF) under the Biological Sciences program (CFDA 47.074) provides $369,297 to San Francisco State University (SFSU) to develop a synthetic transhydrogenation module to enhance the production of valuable chemicals using engineered organisms. The key objectives are to: Elucidate the structural mechanisms underlying the autoregulation of the GltAB-GudB enzyme complex in Bacillus subtilis, which regulates glutamate metabolism and impacts...
- This National Science Foundation (NSF) Engineering program (CFDA 47.041) Project Grant of $545,000 awarded May 15, 2025, supports research to enhance recombinant protein production in Komagataella pastoris (Pichia pastoris), a food-safe yeast widely utilized in biomanufacturing and biological research. The research will deliver scientific outcomes demonstrating how codon usage patterns can be exploited to maximize recombinant protein expression while simultaneously preserving host cellular...
- Via Biofuels Inc. received a $175,000 Project Grant award on September 1, 2025, from the Institute of Bioenergy, Climate, and Environment under the Small Business Innovation Research (SBIR) Program / Small Business Technology Transfer (STTR) Program (CFDA 10.212), administered by the U.S. Department of Agriculture's National Institute of Food and Agriculture (NIFA). The project, scheduled for completion by April 30, 2026, focuses on developing a genetically engineered yeast strain capable of...
DIASTATIC STRAINS OFBREWER'S YEASTPRODUCE AN ENZYME CAPABLE OF BREAKING DOWN RESIDUAL STARCH INTO FERMENTABLE SUGARS.WHEN DIASTATIC CONTAMINATION OCCURS DURING BEER PRODUCTION OR STORAGE, EXTENDEDFERMENTATION OFTEN RESULTS IN NEGATIVE SENSORY IMPACTS SUCH AS DRY MOUTHFEEL, FOAMINESS, INCREASED ALCOHOL CONCENTRATION AND OFF-FLAVORS. IN EXTREME CASES, EXCESSIVE PRODUCTION OF CARBON DIOXIDE AFTER BOTTLING CAN ALSO LEAD TO GUSHING OR EXPLODING BOTTLES AND CANS, PUTTING CONSUMERS IN DANGER.CONTAMINATION BY DIASTATIC YEAST IS AMONG THE LEADING CAUSES OF SPOILAGE IN THE BREWING INDUSTRY AND HAS BEEN ATTRIBUTED TO A LARGE AMOUNT OF WASTE AND PROFIT LOSSESFOR BREWERIES ACROSS AMERICA. IN 2016, COLORADO-BASED BREWERY LEFT HAND BREWING COMPANY RECALLED OVER 20,000 CASES OF BEER DUE TO DIASTATIC CONTAMINATION ALLEGEDLY SOURCED FROM THEIR YEAST SUPPLIER, RESULTING IN A $6M LAWSUIT.TO AVOID SIMILAR FINANCIAL BURDENS AND PR NIGHTMARES, BREWERIESAND YEAST DISTRIBUTORS UTILIZE MOLECULARDIAGNOSTIC ASSAYS FOR DIASTATIC YEAST CHARACTERIZATION. UNFORTUNATELY,CURRENT ASSAYS PROVIDE LIMITED INFORMATION ANDFAIL TO ACCURATELY PREDICT SPOILAGE POTENTIAL ASSOCIATED WITH THESE STRAINS.THROUGH AN IN-DEPTH GENETIC CHARACTERIZATION OF DIASTATIC BREWING STRAINS, THEFACTORS THAT CONTROL DIASTATIC ACTIVITY WILL REVEALED.THE RESULTS OFTHIS RESEARCH PROJECT WILL PROVIDECRITICALINFORMATION NEEDED TO(1)DEVELOPBETTER TOOLS FOR PREDICTING SPOILAGE POTENTIAL IN BREWING STRAINS AND (2)TAKE PREVENTATIVE MEASURES TOWARDS LIMITING SPOILAGE WHENTHESESTRAINS ARE PRESENT.
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