Whole Genome Sequencing from Rainbow Trout blood samples
Closed Solicitation Posted
- Solicitation number
- 1232SA26Q115
- Agency
-
Agricultural Research Service
Department of Agriculture
- Responses due
- Set-aside
- Total Small Business
Opportunity facts
- NAICS code
- 541380 Testing Laboratories and Services
- Place of performance
- Kearneysville, West Virginia 25430, United States
Notice details come from SAM.gov. Updated .
About this opportunity
The Department of Agriculture Agricultural Research Service seeks whole genome sequencing services for three rainbow trout blood samples, with quotations due August 14, 2026, at 9:00 AM CST. This procurement is a Total Small Business Set-Aside with a $19 million size standard.
Place of performance is Kearneysville, West Virginia. The scope encompasses three primary sequencing tasks: PacBio Revio DNA sequencing requiring high molecular weight DNA extraction, CCS library preparation, and delivery of at least 180 Gb of pass-filter reads (Q30) per sample with an N50 read length of at least 12 Kb; Illumina NovaSeq X sequencing of whole-genome unamplified fragment libraries requiring a minimum of 86.4 Gbases output per sample (576M paired-end reads) with 2x150 nucleotide reads and greater than 85% of bases at QC30; and Hi-C library construction using Dovetail Hi-C Kit or similar with Illumina NovaSeq X sequencing requiring a minimum of 150 Gbases output per sample (1,000M paired-end reads). Deliverables are due by September 30, 2026, with all results provided via secure portal download or external hard drive to Dr. Yniv Palti within 60 days of sample receipt. Unused blood and extracted genomic DNA samples must be returned on dry ice.
Quotations must include firm fixed price on company letterhead with place of manufacture, SF-1449 with all contract line items priced in full, SAM.gov Unique Entity ID, and capability statement or technical documentation demonstrating compliance with salient characteristics. Price validity must be at least 60 days after receipt. Evaluation follows lowest price technically acceptable criteria, weighting price, technical acceptability (schedule compliance and detailed explanations of any unachievable requirements), and past performance (acceptable, neutral, or unacceptable ratings). The NAICS code is 541380 (Testing Laboratories and Services); PSC code is B516 (Special Studies/Analysis—Animal/Fisheries). Delivery is FOB destination.
Notice text
Description -
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q1115 is issued as a Request for Quotation (RFQ) to conduct Genome sequencing in rainbow trout.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380. The small business size standard is $19M. This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency.
See attachment “C04_Issued Solicitation_1232SA26Q1115” for applicable clauses, instructions, and evaluation criteria.
In order to be considered for award OFFERORS SHALL SUBMIT ALL OF THE FOLLOWING:
- Submit Firm Fixed Price quotation (quote on company letterhead) detailing the applicable item(s), description, price, PLACE of MANUFACTURE, and inclusive of ALL costs including , but not limited to shipping, and valid for at least 60 days after receipt of quote.
- Submit attached SF-1449 with blocks 17a, 30a, 30b, 30c, and individual Contract Line Items priced in full.
- Submit Sam.gov Unique Entity ID (UEI) with quotation.
- Submit Capability Statement and/or technical documentation/brochures showing technical capability to meet or exceed ALL salient characteristics in the Specifications.
REJECTION OF QUOTATION: Failure to demonstrate compliance or to submit ALL items requested in solicitation prior to due date will be cause to reject the quote without further discussions.
DISCUSSIONS: The Government intends to award without discussions but reserves the right to conduct discussions should it be deemed in the Government's best interest.
Quotes are due on 08/14/2026 at 9:00 AM CST by email to Katelyn Nelson at katelyn.nelson@usda.gov. You must include the solicitation Title Name and Number as the subject line of the email.
Questions-All questions must go to Katelyn Nelson, whom may be reached at Katelyn.nelson@usda.gov for specific questions regarding this combined solicitation. You must include the solicitation name AND number as the subject line of the email. Questions by interested parties regarding the requirement must be submitted by 08/05/2026 by 9:00 AM CST.
Files
| File | Type | Posted |
|---|---|---|
| Sol_1232SA26Q1115.pdf |
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