Project Grant R56HL173608

Award Date 9/24/24
Completion Date 8/31/25
Dollars Obligated $507K
Federal Grant Program
93.837
Assistance Type
Project Grant
Place of Performance
Pittsburgh, PA 15222, USA
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This Project Grant award from the National Heart, Lung, and Blood Institute (NHLBI) under the Cardiovascular Diseases Research program (CFDA 93.837) provides $506,984 to the University of Pittsburgh to investigate the role of the LRP1 gene in the development of congenital heart defects. The research aims to:

  1. Examine how LRP1 deficiency in cardiac neural crest cells and their migratory pathways impacts outflow tract alignment and atrioventricular cushion development using in vitro and in vivo techniques.

  2. Investigate the cellular and molecular mechanisms by which LRP1 deficiency leads to outflow tract and atrioventricular valve defects through single-cell RNA sequencing and analysis of endocytic trafficking.

  3. Generate CRISPR gene-edited mouse models to study the clinical relevance of specific LRP1 mutations identified in human congenital heart disease patients.

The award period runs from September 2024 through August 2025. This research seeks to provide novel insights into the genetic and developmental origins of congenital heart defects, a prevalent birth defect with high morbidity and mortality.

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