This $470,575 Project Grant award from the National Institute of Biomedical Imaging and Bioengineering (NIBIB), under the CFDA 93.286 Discovery and Applied Research for Technological Innovations to Improve Human Health program, supports research at Harvard Medical School to develop programmable human artificial chromosomes (HACs) for cell-based therapeutics, diagnostics, and screening. The key objectives are to: 1) program the HAC to induce expression of therapeutic proteins in response to...
This Project Grant award from the National Heart, Lung, and Blood Institute (NHLBI), under the Cardiovascular Diseases Research program (CFDA 93.837), aims to develop a flexible bioelectronic microarchitecture that integrates a capillary-like delivery network and a tissue-like sensing network to improve cardiac tissue engineering. The $488,026 award supports the creation of a system that can efficiently deliver media and provide real-time physiological feedback to enhance the development of...
This $588,426 Project Grant award, provided by the National Institutes of Health (NIH) Office of the Director under the Trans-NIH Research Support program (CFDA 93.310), aims to investigate how transcription factors efficiently locate and bind to specific DNA binding sites within the crowded nucleus of living cells. The project will employ advanced microscopy techniques to precisely track the 3D diffusion, DNA interactions, and target site discrimination of transcription factors involved in...
This $1,000,000 National Science Foundation project grant supports research at the University of Wisconsin-Madison to develop single cell-level programming of human induced pluripotent stem cell differentiation into chamber-specific cardiomyocytes. The goal is to generate cardiomyocytes from each chamber of the heart through precise control of Wnt and retinoic acid signaling at the single cell level via engineered genetic programs and superstructures. If successful, this approach could establish...
This National Science Foundation (NSF) Engineering Program (CFDA 47.041) Project Grant award of $600,000 to Dartmouth College aims to develop methodologies for efficiently manufacturing mature human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to accelerate drug development and cardiac disease modeling. The project will employ biochip design, machine learning, developmental biology, and tissue engineering approaches to enhance the structural and functional maturity of...
This $600,000 Project Grant award from the National Science Foundation's Division of Information and Intelligent Systems (CFDA #47.070 - Computer and Information Science and Engineering) supports the Massachusetts Institute of Technology (MIT) in developing a cyber physical system to remotely control cellular processes and create synthetic pancreatic tissue. The research aims to overcome challenges in recreating complex 3D tissue structures by using swarms of microrobots to regulate the...
This Project Grant award from the National Heart, Lung, and Blood Institute (NHLBI) under the Cardiovascular Diseases Research program (CFDA 93.837) is focused on developing a scalable manufacturing process for producing designer red blood cells (RBCs) derived from induced pluripotent stem cells (iPSCs). The $568,264 award to SAFI Biotherapeutics Inc., a pre-clinical blood cell therapy company, aims to optimize the iPSC-derived RBC (iRBC) production and validate the performance of different iRBC...
This Project Grant awarded by the National Heart, Lung, and Blood Institute (NHLBI) under CFDA 93.837 - Cardiovascular Diseases Research provides $590,283 to the University of Wisconsin - Madison to develop feedback-controlled closed-loop manufacturing processes for producing cardiomyocytes (heart cells) derived from human pluripotent stem cells. The goal is to improve the robustness and reproducibility of stem cell differentiation across 2D and 3D manufacturing platforms, addressing key...
This federal Project Grant award of $2,249,307, provided by the National Heart Lung and Blood Institute (CFDA 93.837 - Cardiovascular Diseases Research), aims to study the premature maturation arrest of pluripotent-derived cardiomyocytes (PSC-CMs) and develop strategies to overcome this issue. The project will leverage a high-resolution single cell transcriptome map of endogenous cardiomyocyte maturation to identify a critical gene regulatory network underlying the maturation failure in PSC-CMs....
This $500,000 Project Grant from the National Science Foundation's Biological Sciences program (CFDA 47.074) will support the University of Notre Dame's research exploring the differentiation of stem cells into patterned blood vessel networks using hybrid memristor technology. Over a four-year period from October 2022 to September 2026, the University will engineer the first autonomous hybrid memory resistor and cell culture device to control human pluripotent stem cell differentiation into...
The National Institute of Biomedical Imaging and Bioengineering (NIBIB) has awarded a $600,000 Project Grant (CFDA 93.286 - Discovery and Applied Research for Technological Innovations to Improve Human Health) to the Massachusetts Institute of Technology (MIT) to develop synthetic genetic controller circuits that can precisely regulate the expression of key transcription factors involved in directing the differentiation of human induced pluripotent stem cells (hiPSCs) into hemogenic endothelial cells (HECs). The goal is to create an efficient, repeatable, and robust hiPSC-to-HEC conversion protocol that can help enable the clinical translation of hiPSC-derived blood cell products. The project will involve three aims: 1) creating genetic circuit designs to set optimal transcription factor levels, 2) developing circuits to prevent epigenetic silencing, and 3) engineering an autocatalytic RNA sense-and-respond system to autonomously enforce the optimal transcription factor levels. MIT will collaborate with Boston Children's Hospital on this project under a $66,507 sub-award to support the discovery and applied research activities.