Project Grant R43HG011679
- This Project Grant award from the National Science Foundation (NSF) under the Technology, Innovation, and Partnerships (TIP) program (CFDA 47.084) is providing $275,000 to Dinya DNA Inc. to support the commercial development of a novel DNA synthesis technology. The key products or services being delivered include: Developing new methods to ensure higher fidelity DNA precursors, which are the building blocks for DNA synthesis Reducing synthesis costs by streamlining the DNA synthesis reactions...
- This Project Grant award from the National Human Genome Research Institute (CFDA 93.172 - Human Genome Research) provides $452,375 to The Trustees of Columbia University in the City of New York to develop a platform for rapid and efficient generation of multi-site sequence variants using template-guided gene synthesis. The project aims to advance this synthesis approach by optimizing predictive algorithms, experimental protocols, and robotic automation to produce kilobase-sized DNA constructs up...
- This Project Grant, awarded by the National Science Foundation's Mathematical and Physical Sciences Federal Grant Program (CFDA 47.049), provides $583,025 to Professors Shiyue Fang and Yinan Yuan of Michigan Technological University to develop new chemical technologies for the synthesis of long oligonucleotides (DNA and RNA segments). The research aims to overcome limitations of current biological methods and enable cost-effective production of long oligonucleotides essential for applications in...
- This federal Project Grant award, funded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), supports the development of new reagents and methods for synthesizing oligophosphate constructs - phosphorylated biomolecules that play essential roles in human physiology and health. The $444,262 award, effective June 1, 2025 through March 31, 2030, will enable the Massachusetts Institute of Technology (MIT) to: Develop...
- The National Human Genome Research Institute (NHGRI) awarded a $291,232 Project Grant to Micropure Genomics Inc. on September 17, 2025 under the Human Genome Research Federal Grant Program (CFDA 93.172). The project aims to develop an automated, bead-less, and gel-less microfluidic size selection approach to simplify workflows, reduce costs, and enhance the performance of long-read DNA sequencing technologies such as PacBio and Oxford Nanopore. The proposed innovation, called mPREP, leverages...
- The National Institute of General Medical Sciences (NIGMS) awarded a $305,892 Project Grant under the Biomedical Research and Research Training program (CFDA 93.859) to Dotquant LLC, a biotechnology research and development company, to develop a new in vitro transcription kit for high-yield synthesis of chemically modified RNA. The project aims to combine the benefits of wild-type T7 RNA polymerase, which has high efficiency and low cost, with the ability of mutant T7 RNA polymerases to...
- The National Institutes of Health (NIH) awarded a $1,440,000 Project Grant under the Trans-NIH Research Support program (CFDA 93.310) to Baylor College of Medicine to develop two new classes of small, cell-permeable genome regulators. The key objectives are to: (1) create bifunctional polyamides capable of editing a tandem repeat of interest in cell and animal models, (2) develop polyamides to recognize 15-20 base pair motifs occurring only once in the human genome, and (3) democratize these...
- The National Human Genome Research Institute (NHGRI), under the Human Genome Research Federal Grant Program (CFDA 93.172), awarded a $384,418 Project Grant to Xgenomes Corp. on September 25, 2024 to develop a revolutionary DNA sequencing platform. The project aims to create a super-resolution single-molecule sequencing technology that can read short sequence "words" rather than individual nucleotides, enabling one or more orders of magnitude improvements in accuracy and cost over...
- The National Institute of General Medical Sciences (NIGMS) awarded Epicypher, Inc. a $1,264,176 project grant under the Biomedical Research and Research Training federal grant program (CFDA 93.859) to develop a novel toolbox for studying how asymmetric post-translational modifications (PTMs) on nucleosomes regulate chromatin biology. The key products and services to be delivered include: A novel manufacturing method to efficiently generate asymmetric designer nucleosomes (A-dNucs) at...
- The National Human Genome Research Institute (NHGRI), under the Human Genome Research Federal Grant Program (CFDA 93.172), awarded Evizia Inc. a $1,234,692 project grant on April 7, 2025. The grant supports the development of a novel biomolecule desktop imaging system called PRECYSE, which uses high-speed atomic force microscopy (HSAFM) to provide improved quality control for next-generation sequencing (NGS) sample preparation. The PRECYSE system can rapidly size DNA samples from 100 base...
This Project Grant award from the National Institutes of Health's National Human Genome Research Institute provided $768,678 to Molecular Assemblies, Inc. to develop a fully enzymatic oligonucleotide synthesis cycle using engineered template-independent polymerases and a novel phosphate dNTP blocking group. The award supports the goals of the Human Genome Research program (CFDA 93.172) to accelerate genome research and its application to human health. Specifically, the awardee will engineer the template-independent polymerase Terminal Deoxynucleotidyl Transferase to improve incorporation of 3'-O-phosphate dNTPs, enabling fully enzymatic DNA synthesis. They will conduct rational protein design and in silico bioprospecting to generate targeted libraries of the enzyme, then screen libraries using established high-throughput assays to identify variants with improved activity. If successful, the optimized enzymatic clean-up and deblocking steps would allow a proof-of-concept DNA synthesis using the lead variant(s) and 3'-O-phosphate nucleotides. Knowledge gained from this research could guide further enzyme improvements towards longer, lower-error rate DNA synthesis to potentially replace chemical DNA manufacturing methods. The period of performance is from August 16, 2021 to January 31, 2022.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
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| Not listed | $0 | 7/25/22 | ||
| Not listed | $0 | 7/25/22 | ||
| Not listed | $0 | 5/26/22 | ||
| Not listed | $0 | 5/26/22 | ||
| Not listed | $0 | 5/26/22 |