Project Grant R35GM158437

Award Date 6/1/25
Completion Date 3/31/30
Dollars Obligated $444K
Federal Grant Program
93.859
Assistance Type
Project Grant
Place of Performance
Cambridge, MA 02139, USA
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This federal Project Grant award, valued at $444,262 and provided by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training Program (CFDA 93.859), supports the development of innovative reagents and methods for synthesizing oligophosphate constructs. The key products and services to be delivered include:

  1. New methods and reagents for synthesizing oligophosphate chains of increasing length, including pentaphosphate, hexaphosphate, and longer chains. These will serve as chemical probes to enable the exploration of the "polyp-ome" - the cellular landscape of protein oligophosphorylation, an important post-translational modification.

  2. Strategies for covalently linking organic molecules, such as nucleosides, to these oligophosphate chains, including the development of non-hydrolyzable phosphorus-carbon bonds. This will facilitate the creation of potent small molecule inhibitors of enzymes like RNase A.

  3. New methods for attaching "clickable" moieties to the oligophosphate chain ends, enabling the conjugation of these constructs to peptides or proteins. This will support an exciting collaborative project to decorate protein surfaces with oligophosphate groups for packaging into lipid nanoparticles for cellular delivery.

The award was granted to the Massachusetts Institute of Technology (MIT) and will run from June 1, 2025 to March 31, 2030, advancing fundamental research on oligophosphate biomolecules with broad applications in biochemistry, chemical biology, and drug development.

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