The National Institute of Allergy and Infectious Diseases (NIAID) awarded a $229,411 Project Grant (CFDA 93.855 - Allergy and Infectious Diseases Research) to Northwestern University to investigate Neisseria gonorrhoeae double-stranded bacteriophage. The research aims to determine whether one or more phage islands can produce phage, explore the regulation of two essential LexA orthologs, and assess whether these phage(s) can infect Neisseria strains or species. This project builds upon the...
This federal Project Grant award, totaling $177,467.00, was provided by the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855). The award aims to examine the role of epigenetic changes in the survival and replication of the pathogen Legionella pneumophila, which causes the disease Legionnaire's Disease. The key objectives of this research project are to: 1) Investigate the DNA methylation patterns in...
This Project Grant award from the National Institute of Allergy and Infectious Diseases (NIAID), under the Allergy and Infectious Diseases Research program (CFDA 93.855), provides $256,995.00 to Tufts University School of Medicine to develop a novel method for quantitatively linking host cell mutations to specific bacterial pathogen traits. The goal is to overcome limitations in current approaches for identifying host proteins that promote or restrict the growth of intracellular pathogens like...
This federal Project Grant award from the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855) provides $506,394.00 to the University of Chicago to investigate the role of bacterial cholesterol-dependent cytolysins (CDCs) in activating the NLRP3 inflammasome, a key innate immune signaling complex. The research aims to define the molecular and cellular mechanisms by which CDCs can reorganize host organelles to...
The National Institute of Allergy and Infectious Diseases (NIAID) awarded a $147,656 Project Grant under the Allergy and Infectious Diseases Research program (CFDA 93.855) to St. Jude Children's Research Hospital Inc. The grant supports research to determine: 1) the specificity underlying mucin-mediated autolysis inhibition in Streptococcus pneumoniae; 2) the impact of mucin-mediated autolysis inhibition on pneumococcal cytotoxicity; and 3) the genetic determinants required for prolonged...
This $2,734,799 Project Grant from the National Institute of Allergy and Infectious Diseases (NIAID), under the Allergy and Infectious Diseases Research program (CFDA 93.855), supports research on the respiration and fitness of Bacteroides bacteria in the human gut. The key objectives are to study the NUO respiratory complex, menaquinone biosynthesis, and the NRFHA complex in Bacteroides to better understand their energy generation processes and how they support bacterial fitness in the gut...
This $453,900 Project Grant was awarded by the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855). The grant is funding research to develop a genome editing platform using broadly functioning phage recombinases, which can be used to genetically manipulate a wide range of bacterial species, including clinically relevant pathogens. The goal is to enable direct investigation of the genetic elements driving bacterial...
This federal Project Grant award for $637,275 from the National Institute of Allergy and Infectious Diseases (NIAID) under the Allergy and Infectious Diseases Research program (CFDA 93.855) supports research to uncover the mechanisms behind how gram-negative bacterial pathogens rapidly develop tolerance to chemical stressors like antibiotics. The research aims to determine how bacteria can inhibit the functioning of existing porin proteins in their outer membrane within minutes of exposure to...
The National Institute of Allergy and Infectious Diseases (NIAID) awarded a $637,729 Project Grant (CFDA 93.855 - Allergy and Infectious Diseases Research) to President and Fellows of Harvard College (Harvard Medical School) to investigate the molecular mechanisms underlying the activation and regulation of essential bacterial cell wall synthase enzymes. The research aims to develop a detailed mechanistic understanding of these enzymes, which are critical for bacterial growth and potential...
The National Institute of Allergy and Infectious Diseases (NIAID) awarded a $125,160 Project Grant under the Allergy and Infectious Diseases Research program (CFDA 93.855) to The Ohio State University to characterize a novel bacterial immune system with unknown mechanisms. The grant will fund research to define the structure and assembly mechanisms of a two-component system, named A and B, that forms a large complex for anti-phage defense. The project will utilize cryogenic electron microscopy...
The National Institute of Allergy and Infectious Diseases (NIAID) awarded a $456,000 Project Grant (CFDA 93.855 - Allergy and Infectious Diseases Research) to Northwestern University to conduct research on how the CAS2 ribonuclease protein in Legionella pneumophila (the bacterium that causes Legionnaires' disease) non-canonically controls the pathogen's thermal tolerance and virulence. The research aims to determine if CAS2 acts directly by binding and cleaving/degrading a portion of the HSPC2 mRNA transcript to increase its stability, or indirectly by binding and degrading a repressor or activator of HSPC2 transcription. Findings from this project have the potential to advance the understanding of L. pneumophila physiology and virulence, define a new mode of bacterial gene regulation controlled by CAS2 proteins, and uncover novel nuclease activities with broad applications. The project period runs from July 2024 to May 2026.