Project Grant R01HD114591
- This $2,624,970 federal Project Grant, awarded by the National Institutes of Health (NIH) under the Trans-NIH Research Support program (CFDA 93.310), aims to construct the first-ever foundational in silico (computational) model of whole-embryo mouse embryogenesis. The project will leverage cutting-edge sequencing technology and machine learning techniques to establish a large-scale 3D multi-omics cell atlas of mouse embryogenesis from embryonic day 6.5 to 16.5, involving a total of 50 million...
- This $1,737,000 Project Grant awarded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859) supports the development of innovative single-cell genomics technologies for joint analysis of regulatory dynamics and transcriptional states. Key objectives include: Developing multiomics tools to measure rates of epigenomic changes (DNA methylation, demethylation, oxidative damage, DNA repair) and relate these to...
- This two-year, $1,204,512 National Science Foundation project grant supports research into the robustness and adaptability of the dynamic epigenome through a multiscale approach. Funded under the Biological Sciences program (CFDA 47.074), the award to Brandeis University involves investigating the fundamental rules that guide adaptive epigenetic changes in cells without alterations to the genetic blueprint. The research utilizes high-resolution imaging, microfluidics, and automated culture...
- Federal Grant Award Summary Icahn School of Medicine at Mount Sinai received a $462,454 Project Grant award from the National Institutes of Health (NIH) Office of the Director under the Trans-NIH Research Support program (CFDA 93.310), effective August 8, 2025 through June 30, 2027. The award supports research investigating DNA methylation (5-methylcytosine) dynamics in Down syndrome (trisomy 21) hematopoiesis and leukemia development. The primary deliverable involves mapping epigenetic...
- This federal Project Grant award for $378,123 from the National Institute of Child Health and Human Development (NICHD) under the Child Health and Human Development Extramural Research program (CFDA 93.865) supports research to identify and test mechanisms that establish transcriptional regulatory potency in early embryos. The primary activities include using genomic methods like PRO-seq, DREG, and OMNI-ATAC to characterize transcriptionally active regulatory elements, DNA accessibility, and...
- This Project Grant, awarded by the National Science Foundation's Biological Sciences program (CFDA 47.074), provides $900,000 in funding to The Regents of the University of Colorado from February 1, 2025 to January 31, 2028. The project focuses on uncovering the molecular mechanisms by which the DNA methyltransferase 1 (DNMT1) enzyme regulates gene silencing and the inheritance of distinct cell identities. The research will utilize structural biology, biochemistry, and cell biology methods to...
- Grant Award Summary Children's Mercy Hospital received a $681,829 Project Grant from the National Institute of Child Health and Human Development (NICHD) under the Child Health and Human Development Extramural Research program (CFDA 93.865), effective August 15, 2025, through April 30, 2030. The award supports the development of a comprehensive map of human imprinted genes—the "imprintome"—using advanced 5-methylcytosine high-fidelity genome sequencing (5MC-HiFi-GS) technology. This...
- This Project Grant award of $442,750, funded by the National Institute of General Medical Sciences (NIGMS) under the Biomedical Research and Research Training program (CFDA 93.859), supports fundamental research into the regulation of histone lysine methyltransferase (HLMT) substrates. Awarded to Virginia Polytechnic Institute & State University on August 1, 2025, with a completion date of June 30, 2030, the research addresses critical gaps in understanding how cells maintain epigenetic...
- Federal Project Grant Award Summary The National Human Genome Research Institute (NHGRI) awarded The Regents of the University of California, San Francisco a Project Grant totaling $135,206 under the Human Genome Research program (CFDA 93.172), effective September 1, 2025 through August 31, 2027. This award supports the development of single-cell functional genomic technologies designed to investigate DNA methylation changes associated with human diseases. The project addresses a critical gap in...
- This $148,568 federal Project Grant awarded by the National Institute of General Medical Sciences (NIGMS) under CFDA 93.859 Biomedical Research and Research Training Program will support research to elucidate the role of chromatin structures in genomic imprinting. The project, led by Harvard University, aims to investigate the regulatory mechanisms and functional implications of allele-specific chromatin structures in the regulation of the MEST-COPG2 imprinted domain, which is associated with...
DNA METHYLATION DYNAMICS OF MAMMALIAN DEVELOPMENT - PROJECT SUMMARY THE PROGRESSION FROM A PLURIPOTENT STATE INTO THE MYRIAD CELL TYPES THAT ARISE DURING MAMMALIAN DEVELOPMENT INVOLVES A HOST OF EPIGENETIC PROPERTIES THAT DICTATE GENE EXPRESSION PATTERNS AND ENFORCE THE COMMITMENT OF A CELL TO A SPECIFIC LINEAGE. THE ADVENT OF SINGLE-CELL GENOMICS HAS ENABLED THE STUDY OF CELL LINEAGE DYNAMICS AT UNPRECEDENTED RESOLUTION; HOWEVER, SUCH STUDIES HAVE BEEN LARGELY LIMITED TO RNA TRANSCRIPTION. THE DYNAMIC MODULATION OF THE EPIGENOME THAT GOVERNS THESE PROCESSES INVOLVES MULTIPLE LAYERS OF REGULATION, INCLUDING A MAJOR ROLE BY DNA METHYLATION - A CANONICALLY REPRESSIVE PROPERTY THAT CAN ACT AS A GATEKEEPER OF GENOMIC PERMISSIVENESS. ABERRANT DNA METHYLATION IS ALSO ASSOCIATED WITH NUMEROUS DEVELOPMENTAL DISORDERS AND DISEASES; HOWEVER, IT IS ALSO ONE OF THE LEAST STUDIED PROPERTIES, PARTICULARLY AT THE SINGLE-CELL LEVEL, PRIMARILY DUE TO TECHNOLOGICAL LIMITATIONS. WE HAVE RECENTLY ADDRESSED THIS SHORTCOMING WITH THE DEVELOPMENT OF A HIGH- THROUGHPUT SINGLE-CELL DNA METHYLATION ASSAY CAPABLE OF PRODUCING 100'S OF THOUSANDS OF CELL PROFILES IN A SINGLE EXPERIMENT, PAVING THE WAY FOR STUDIES OF THIS PROPERTY WITH A POWER COMPARABLE TO THAT OF TRANSCRIPTION. HERE WE PROPOSE THE PRODUCTION OF AT ATLAS OF DNA METHYLATION DURING MAMMALIAN EMBRYONIC DEVELOPMENT IN THE MOUSE AT 24-HOUR INCREMENTS FROM EMBRYONIC DAY 8.5 TO 17.5 IN ORDER TO PRODUCE A GRANULAR MAP OF DNA METHYLATION DYNAMICS DURING KEY STAGES OF LINEAGE PRIMING AND COMMITMENT. WE WILL FURTHER ASSESS THE INTESTINAL EPITHELIUM USING NOVEL MULTIOMIC TECHNOLOGIES IN ORDER TO GAIN MECHANISTIC INSIGHT INTO HOW LAYERS OF EPIGENETIC CONTROL DRIVE AND ENFORCE ONE ANOTHER. THESE DATA WILL ENABLE THE ASSESSMENT OF STANDING HYPOTHESES REGARDING THE ROLE OF DNA METHYLATION IN GENOMIC PERMISSIVENESS AND CELL FATE DECISIONS. FINALLY, THE DATASETS, WHICH WILL REPRESENT A POWERFUL RESOURCE FOR DEVELOPMENTAL BIOLOGY AND EPIGENOMIC STUDIES, WILL BE MADE OPENLY AVAILABLE THROUGH INTERACTIVE PORTALS TO ENABLE BROAD ACCESS BY THE GREATER RESEARCH COMMUNITY.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $653.7k | 7/18/25 | ||
| Not listed | $654.4k | 7/25/24 |