Project Grant K99GM164667
- The National Institute of General Medical Sciences awarded the University of Florida $386,985 on July 23, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to conduct a five-year investigation into the structure-function relationships of single-stranded DNA (ssDNA) in biological systems. The research will develop methods to probe structured ssDNA in cells, with initial focus on two biomedically relevant lines of investigation. The first examines complex ssDNA...
- The National Institute of General Medical Sciences awarded the University of Arkansas for Medical Sciences $1,455,158 on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) for Project Grant R01GM165736, titled "DNA Helicases: Mechanisms and Regulation." The award funds research into the mechanisms and regulation of DNA helicases—molecular motors critical to nucleic acid processing. Mutations in helicases are associated with genomic instability,...
- The National Institute of General Medical Sciences awarded the University of Florida $383,750 on April 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to conduct basic research on post-translational modification systems in archaea, with performance through January 31, 2031, at Gainesville, Florida. The project advances understanding of how post-translational modifications coordinate to regulate stress response in the archaeon Haloferax volcanii. Three...
- The National Institute of General Medical Sciences awarded the University of Oregon $920,642 on March 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to support research on the molecular mechanisms of DNA breathing fluctuations and their roles in the assembly, function, and control of macromolecular complexes that direct DNA replication in bacteriophage T4. The award funds research conducted by the collaborative groups of Professors Andrew H. Marcus and Peter...
- The National Institute of General Medical Sciences awarded the University of Florida $398,545 on September 3, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate how active and silenced transposable elements shape meiotic recombination in maize and rice. The research will generate high-resolution crossover maps and perform genome-wide assessment of recombination frequency near transposon insertions, examine whether transposon-derived double-strand...
- The National Institute of General Medical Sciences, National Institutes of Health, awarded the University of Florida $422,125 on August 15, 2026, to develop rapid, wash-free fluorescent signal-switching methods for high-plex RNA imaging in cells and tissues under the Biomedical Research and Research Training program (CFDA 93.859). The research addresses limitations in current multiplexed fluorescence imaging by establishing a DNA displacement reaction-based signal exchange (DIRSE) platform...
- The National Institute of General Medical Sciences awarded The University of Texas Health Science Center at San Antonio $433,125 on June 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to support research into the spatiotemporal regulation of DNA metabolism pathways. The project examines how homologous recombination proteins are selectively activated and deactivated at DNA lesions and replication fork structures, with particular focus on tyrosine...
- The National Institute of General Medical Sciences awarded the Regents of the University of Minnesota $386,879 on May 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate the mechanisms governing nucleosome dynamics and epigenetic inheritance during cell division. The research examines how parental histones transfer to newly replicated DNA strands during DNA replication and how this transfer regulates replication fork recovery under stress conditions....
- The National Institute of General Medical Sciences awarded the University of North Carolina at Chapel Hill $533,425 on July 15, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to define new roles of the E3 ligase RNF25 in DNA replication and stress signaling. The research addresses DNA replication stress—a slowing of DNA synthesis that threatens genome integrity—by investigating how cells mount DNA damage response signaling to coordinate resolution of stalled...
- The National Institute of General Medical Sciences awarded the University of Virginia $444,125 on March 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to investigate the mechanisms of genome integrity maintenance and break-induced replication at repetitive DNA sequences. The research focuses on elucidating how break-induced replication drives genome instability in repetitive DNA regions, particularly telomere maintenance through the alternative lengthening...
The National Institute of General Medical Sciences awarded the University of Florida $114,100 on September 1, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to support basic research on the functional dynamics and regulatory mechanisms of RAD3/XPD-family helicases during DNA replication stress. The research investigates how helicases are modulated by protein-protein interactions to maintain replication fork integrity when cells encounter DNA lesions and non-canonical DNA structures. The project compares two evolutionarily related helicases—Escherichia coli YoaA and its human ortholog DDX11—to elucidate both conserved and specialized regulatory strategies across prokaryotic and eukaryotic systems. Three specific aims define the work: characterizing interactions between YoaA-X and single-stranded DNA-binding protein (SSB), and between DDX11 and replication protein A (RPA), on canonical and structured DNA substrates; determining how RPA influences DDX11 interaction with the fork protection protein TIM; and investigating how the DDX11-CTF4 interaction, modulated by partner proteins, affects replication fork dynamics and sister chromatid cohesion. The research employs biochemical reconstitution, single-molecule imaging, and yeast cell-based assays. Performance takes place in Gainesville, Florida, with a period of performance from September 1, 2026, through August 31, 2028. The assistance type is a Project Grant.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $114.1k | 8/28/26 |