This $495,516 Project Grant, awarded by the National Science Foundation (NSF) under the Engineering program (CFDA 47.041), aims to develop a next-generation, ultrasensitive, and easy-to-use diagnostic platform for detecting nucleic acids as biological markers for diseases and pathogens. The key innovations focus on: (1) signal amplification through redox-tagged reporter aptamers in a graphene microstructure array; (2) enhancing charge transfer kinetics through plasma-induced graphene functionalization; (3) integrating 3D dual-functional capture electrodes for rapid and multimodal signal readout; and (4) demonstrating the platform for virus DNA detection using a CRISPR-Cas12a assay. The project will advance the understanding of device-material-biomolecule interactions and provide a framework for high-performance, field-deployable biosensors with potential applications in clinical diagnostics, environmental monitoring, food safety, and biodefense. The award period is from October 1, 2025 to September 30, 2028, and will be performed at Penn State University.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $495.5k | 7/9/25 |