This $199,997 federal Project Grant, awarded by the National Science Foundation's Engineering program (CFDA 47.041), aims to improve the stability and sensitivity of a heat-resistant version of the CRISPR-Cas13 enzyme for rapid and accurate detection of disease-causing RNA. The collaborative research project between William Marsh Rice University and its partners will leverage mechanism-based protein engineering and electrochemical devices to generate next-generation RNA detection tools for infectious disease diagnosis. The key objectives are to investigate the structure and mechanism of the thermostable Cas13, engineer superior variants with enhanced thermostability, protease resistance, target sensitivity, and reaction speed, and integrate the optimized Cas13 with innovative electrochemical platforms to enable ultrasensitive and robust RNA detection. The project will also involve educational outreach activities to engage high school and community college students, particularly from underrepresented backgrounds, in biological and bioengineering research. This award, which runs from November 2024 to October 2027, aims to provide superior RNA detection tools for medical and research applications while also advancing the fundamental understanding of the Cas13 nuclease mechanism.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $200.0k | 8/15/24 |