This Project Grant award of $275,000.00 from the National Science Foundation (NSF) under the NSF Technology, Innovation, and Partnerships (CFDA 47.084) program aims to develop a novel platform for accelerated strain development for precision fermentation to enable high-yield production of animal proteins in yeast. The key objectives of this award are to: 1) Develop a high-throughput sequence screening approach to identify high-expression proteins and signal peptides, 2) Train an AI model to...
This Project Grant award of $275,000, funded by the National Science Foundation (NSF) under the CFDA program 47.084 "NSF Technology, Innovation, and Partnerships", aims to develop improved bacterial strains for more cost-effective production of proteins used in detergents, personal care products, and dairy. The project seeks to identify superior bacterial hosts and develop advanced genetic engineering tools to convert these bacteria into efficient protein factories. The goal is to...
This Project Grant award for $1,000,000 from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) aims to develop a polyketide-based platform to produce a range of non-canonical amino acids (NCAAs) and demonstrate their incorporation into proteins expressed by the bacterium Bacillus subtilis. The key objectives of the 3-year project are to: 1) engineer a polyketide synthase (PKS) system to produce threonine and evolve it for increased productivity; 2) utilize...
This $350,000 project grant awarded by the National Science Foundation (NSF) Engineering program (CFDA 47.041) aims to develop a yeast strain with a robust protein secretion system that is tolerant to extreme pH and temperature levels. The primary objectives are to engineer the secretory pathway of the yeast Kluyveromyces marxianus using gene editing tools such as CRISPR, identify critical genes influencing protein production and secretion, and develop effective CRISPR libraries and selection...
This Project Grant award from the National Science Foundation (NSF) under the Biological Sciences program (CFDA 47.074) provides $369,297 to San Francisco State University (SFSU) to develop a synthetic transhydrogenation module to enhance the production of valuable chemicals using engineered organisms. The key objectives are to: Elucidate the structural mechanisms underlying the autoregulation of the GltAB-GudB enzyme complex in Bacillus subtilis, which regulates glutamate metabolism and impacts...
This National Science Foundation (NSF) Project Grant award under the Biological Sciences program (CFDA 47.074) provides $429,656.00 to Cornell University to conduct research using synthetic biology methods to engineer microorganisms to produce valuable plant natural products. The key objectives are to reconstitute plant biosynthetic machinery in yeast in order to better understand and harness plant enzyme complexes for natural product synthesis. This research aims to develop a more resilient...
This Project Grant award from the National Science Foundation's Biological Sciences program provides $509,573 to Trustees of Tufts College, doing business as Tufts University, for a 3-year research project titled "Collaborative Research: Mechanisms of Community Coalescence in Synthetic Microbiomes." The project aims to investigate how microbiomes, such as those found in fermented foods like sourdough and kombucha, reassemble and function when they mix, a phenomenon known as community...
This National Science Foundation (NSF) Engineering Program (CFDA 47.041) Project Grant award of $519,071 to Trustees of Boston University will support research to develop light-controlled metabolic engineering strategies for sustainable bioproduction. The project aims to use optogenetic control techniques to balance the trade-off between production of valuable biochemicals, such as octanoic acid, and normal cell growth in microorganisms. Key objectives include developing novel light-responsive...
The National Science Foundation awarded a $422,834 Project Grant to the University of California Irvine under the Engineering federal grant program (CFDA 47.041) for research titled "Collaborative Research: Data-Driven Engineering of the Thermotolerant Yeast Kluyveromyces Marxianus." The three-year project period runs from October 1, 2022 to September 30, 2025. The award will support applying machine learning and deep learning techniques to metabolic engineering of yeast for...
The National Science Foundation (NSF) awarded a $274,980 SBIR Phase I Project Grant to Cisterna Biologics, Inc. to develop a novel platform for cost-efficient mRNA production in yeast. The project aims to transform yeast cells into efficient mRNA factories and utilize advanced chromatographic techniques for purification, with the goal of reducing mRNA manufacturing costs by 90% compared to current in vitro transcription methods. Key technical objectives include demonstrating stable mRNA fusion...