Project Grant 2323983

Award Date 2/15/24
Completion Date 1/31/27
Dollars Obligated $350K
Federal Grant Program
47.041
Assistance Type
Project Grant
Place of Performance
Irvine, CA 92697, USA
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This $350,000 project grant awarded by the National Science Foundation (NSF) Engineering program (CFDA 47.041) aims to develop a yeast strain with a robust protein secretion system that is tolerant to extreme pH and temperature levels. The primary objectives are to engineer the secretory pathway of the yeast Kluyveromyces marxianus using gene editing tools such as CRISPR, identify critical genes influencing protein production and secretion, and develop effective CRISPR libraries and selection...
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The National Science Foundation (NSF) Engineering program (CFDA 47.041) awarded a $349,999 Project Grant to the University of California Irvine (UC Irvine) to conduct collaborative research on engineering the yeast Kluyveromyces marxianus for enhanced protein secretion. The key objectives are to develop a yeast strain with a robust protein secretion system that is tolerant to extreme pH and temperature levels, and to improve STEM education for underrepresented minorities and non-traditional students. This will be achieved through gene editing tools like CRISPR to identify critical genes influencing protein production and secretion, and leveraging the insights to engineer more efficient protein secretion. The research could result in effective CRISPR gRNA libraries, optimized selection strategies, and a library of gene interventions leading to substantially higher protein secretion for a range of proteins. The award has been deemed worthy of support by NSF based on its intellectual merit and broader impacts.

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