Project Grant 20213352235343

Award Date 9/1/21
Completion Date 8/31/25
Dollars Obligated $500K
Federal Grant Program
10.219
Assistance Type
Project Grant
Place of Performance
Storrs, CT 06269, USA
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The project aims to use CRISPR/Dcas technology to produce site-specific DNA methylation-edited tomato plants and compare them to plants produced using conventional tissue culture and genetic transformation techniques. Whole genome bisulfite sequencing, RNA-seq, fruit quality analyses and other phenotypic assessments will be performed on tomato fruits to conduct a side-by-side analysis of any unintended effects generated from the different technologies. The knowledge generated will aid plant breeders using DNA methylation editing and help inform regulatory policymaking. Outreach efforts will also seek to improve public understanding of gene editing technology.

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