The National Science Foundation awarded a $307,400 Project Grant under its Biological Sciences program (CFDA 47.074) to the University of Missouri System to develop epigenetic editing tools for improving crop plants. The project aims to expand the capabilities of genome editing by enabling targeted changes in gene expression, rather than just DNA sequence, to generate novel traits in important crops like rice and tomato. Key objectives include adapting the epigenetic editing approach...
This Project Grant award in the amount of $1,211,545.00 from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) aims to develop highly efficient and robust Prime Editing (PE) systems in rice, tomato, and poplar plants. PE is a cutting-edge CRISPR/Cas gene editing technology that enables precise replacement, addition, or removal of specific DNA sequences. The project objectives are to optimize PE proteins, improve PE guide RNA structure and expression, and modify...
This federal Project Grant award of $725,512 from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) aims to develop highly efficient and robust prime editing (PE) systems in plants, specifically rice, tomato, and poplar.
The primary objectives are to optimize prime editor proteins, improve prime editing guide RNA (pegRNA) structures and expression, and modify plant DNA repair systems to enhance PE efficiency. The resulting PE tools are expected to facilitate...
This Project Grant from the National Science Foundation's Biological Sciences program (CFDA 47.074) provides $2,379,217 to the Donald Danforth Plant Science Center from March 1, 2022 to February 28, 2025. The grant supports research to develop targeted DNA integration techniques in plants.
Specifically, the Center and its sub-awardees—the University of South Carolina Aiken Campus and University of Missouri—will work to engineer hyperactive versions of the Pong transposase proteins and...
This $322,020 Project Grant award from the National Science Foundation's (NSF) Division of Integrative Organismal Systems will support collaborative research at the University of California, Davis (UC Davis) to develop improved methods for CRISPR/Cas9-based gene editing in maize. The key objectives are to generate maize lines expressing high levels of Cas9 nuclease and to engineer and optimize viral vectors to efficiently deliver guide RNAs for both somatic and heritable gene editing. This...
This $999,999 Project Grant from the National Science Foundation's Biological Sciences program (CFDA 47.074) will fund research at the Donald Danforth Plant Science Center to elucidate the mechanisms initiating de novo chromatin modification cycles in plant cells. Over three years, the award will support experiments using cutting-edge techniques to dissect the molecular triggers establishing heritable DNA methylation patterns and long-term epigenetic silencing of transposable elements and...
This Project Grant, awarded by the National Science Foundation (NSF) under the Biological Sciences program (CFDA 47.074), aims to develop novel methods for plant genome editing using the parasitic plant Cuscuta campestris (dodder). The $450,000 award, effective July 1, 2024 through June 30, 2027, will enable researchers at the University of Missouri System to:
Achieve stable transformation of dodder to enable its use in host gene editing;
Develop a dodder protoplast system for rapid...
This three-year, $1.8 million project grant from the National Science Foundation Division of Integrative Organismal Systems aims to develop rapid transformation and gene editing techniques in maize through the Biological Sciences program (CFDA 47.074). The University of Missouri System will work to establish a system for precisely inserting transgenes and editing genes at predetermined sites in the genome of maize. Researchers will create "landing pads" on five chromosomes of a...
The National Science Foundation (NSF) Division of Integrative Organismal Systems awarded a $277,978 Project Grant under the Biological Sciences program (CFDA 47.074) to the University of California, Berkeley. The 3-year grant, beginning on Sep 1, 2023, aims to develop new tools and resources to enable more efficient gene editing in maize, the world's most produced crop. Specifically, the project will generate maize lines that constitutively express the Cas9 gene editing enzyme and engineer...
This $250,000 federal Project Grant award from the National Science Foundation (NSF) Biological Sciences program (CFDA 47.074) supports research aimed at understanding the molecular mechanisms controlling cell fate decisions during inflorescence (flower-bearing structure) development in panicoid cereal crops like corn, wheat, and rice.
The key objective is to leverage the model grass Setaria viridis to dissect the genetic module involving the RAMOSA1 transcription factor and growth hormones...